Abstract
The proteoglycans of cartilage occur in a form which is readily extracted (soluble) and in form which is relatively difficult to extract (resistant). Following the extraction of the soluble proteoglycans from slices of epiphyses from young rats, the distribution of the resistant proteoglycans are visualized by staining with toluidine blue. Daily quantitative recoveries of uronic acid over 7 days are used as an index of the rate and completeness of extraction. In contrast to other cartilages (nasal, costal, ear, articular) in which the resistant proteoglycans are restricted to perilacunar localizations, the resistant proteoglycans in epiphyseal plate extend across the plate as a continuous stratum and occupy extraterritorial regions. This stratum of resistant proteoglycans is difficult to identify with a specific zone in the plates of young individuals, because of primitive columniation. In more highly organized, older human and porcine epiphyseal plates, however, the stratum is clearly seen at the junction of the zones of resting and proliferating chondrocytes. It dips down a short distance between the columns, disappears and then reappears again at the level of the zone of provisional calcification. These observations are discussed in the context of endochondral growth.
Résumé
Les protéoglycanes du cartilage se présentent sous une forme que l'on peut extraire facilement (soluble) et sous une formule difficile à extraire (résistante). Après extraction de protéoglycanes de coupes d'épiphyses de jeunes rats, le répartition des protéoglycanes résistantes est visualisée par coloration au bleu de toluidine. La détermination quantitative quotidienne d' acide uronique pendant 7 jours est utilisée comme indice de la vitesse et de l'efficacité de l'extraction. Contrairement à d'autres cartilages (nasal, costal, oreille, articulaire) où les protéoglycanes résistantes sont limitées à des régions périlacunaires, les protéoglycanes résistantes de la métaphyse s'étendent au-delà sous forme d'une couche continue et occupent des régions extra-territoriales. Cette couche de protéoglycanes résistantes est difficile d'identifier avec une zone spécifique dans la métaphyse de jeunes individus, par suite d'un alignement primitif. Cependant au niveau de métaphyses humaines ou de porcs plus âgés, cette couche est nettement visible à la jonction des zones de chondrocytes au repos et en division. Elle s'étend sur une courte distance entre les cellules sériées, disparait et réapparait à nouveau au niveau de la zone de calcification temporaire. Ces résultats sont discutés en fonction de la croissance enchondrale.
Zusammenfassung
Die Knorpelproteoglycane kommen in einer leicht extrahierbaren (löslichen) und in einer relativ schwer extrahierbaren (resistenten) Form vor. Nach der Extraktion der löslichen Proteoglycane aus Epiphysenschnitten junger Ratten wird die Verteilung der resistenten Proteoglycane durch Toluidinblau-Färbung aufgezeigt. Als Index für die Geschwindigkeit und Vollständigkeit der Extraktion wird die tägliche quantitative Ausbeute von Uronsäure während 7 Tagen verwendet. Im Gegensatz zu anderen Knorpelarten (Nasen-, Rippen-, Ohren- und Gelenkknorpel), bei welchen die resistenten Proteoglycane nur perilacunär vorkommen, gehen die resistenten Proteoglycane der Epiphysenplatte über die Platte als zusammenhängende Schicht hinaus und treten in extraterritorialen Bereichen auf. Diese Schicht resistenter Proteoglycane kann in den Platten junger Individuen wegen der ursprünglichen Säulenbildung nur schwierig als eine bestimmte Zone identifiziert werden. In höher organisierten, älteren Epiphysenplatten des Menschen und des Schweines ist die Schicht jedoch deutlich an der Berührungsstelle der Zonen ruhender und proliferierender Chondrocyten ersichtlich. Sie setzt sich eine kurze Strecke zwischen den Säulen fort, verschwindet dann aber und erscheint wieder auf der Höhe der vorläufigen Verkalkungszone. Diese Beobachtungen werden mit dem endochondralen Wachstum in Zusammenhang gebracht.
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Campo, R.D. Soluble and resistant proteoglycans in epiphyseal plate cartilage. Calc. Tis Res. 14, 105–119 (1974). https://doi.org/10.1007/BF02060287
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DOI: https://doi.org/10.1007/BF02060287