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Journal of Chemical Ecology

, Volume 20, Issue 7, pp 1445–1455 | Cite as

Ecdysteroids fromPycnogonum litorale (Arthropoda, Pantopoda) act as chemical defense againstCarcinus maenas (Crustacea, Decapoda)

  • K-H Tomaschko
Article

Abstract

Pycnogonum litorale (Ström) is unpalatable to the common shore crabCarcinus maenas, a generalist predator in the pycnogonid's habitat. A feeding bioassay reveals that the crabs are deterred by ecdysteroids that occur in high levels in all developmental stages ofP. litorale. The total ecdysteroids in the pycnogonids reach 5.9×10−4 M. The 20-hydroxyecdysone 22-acetate (20E22A), which is the predominant ecdysteroid in the pycnogonids, and 20-hydroxyecdysone (20E), the arthropod molting hormone, were tested for their antifeedant effect onC. maenas. When contained in food pellets in homogeneous concentrations, 20E and 20E22A significantly reduced food consumption at 1.25×10−4 and 5.0×10−4 molar levels, respectively. The present results demonstrate for the first time chemical defense in arthropods in a marine predator-prey relationship. Furthermore, they provide evidence that ES contained in one animal can act as feeding deterrents on another animal.

Key Words

Pycnogonids Pycnogonum litorale Pantopoda Crustacea Carcinus maenas ecdysteroids 20-hydroxyecdysone 20-hydroxyecdysone 22-acetate chemical defense predator-prey interactions 

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References

  1. Adelung, D., Seifert, P., andBuchholz, F. 1980. Studies on the identification and the action of the sexual pheromone of the shore crabCarcinus maenas.Verh. Dtsch. Zool. Ges. 73:316.Google Scholar
  2. Arnaud, F., andBamber, R.N. 1987. The biology of Pycnogonida.Adv. Mar. Biol. 24:1–96.Google Scholar
  3. Ashburner, M. 1980. Chromosomal action of ecdysone.Nature 285:435–436.PubMedGoogle Scholar
  4. Atema, J., andGagosian, R.B. 1973. Behavioral responses of male lobsters to ecdysones.Mar. Behav. Physiol. 2:15–20.Google Scholar
  5. Bandara, B.M.R., Jayasinghe, L., Karunaratne, V., Wannigama, G.P., Bokel, M., Kraus, W., andSotheeswaran, S. 1989. Ecdysterone from stem ofDiploclisia glaucescens.Phytochemistry 28:1073–1075.Google Scholar
  6. Bergström, J., Stürmer, W., andWinter, G. 1980.Palaeoisopus, Palaeopantopus andPalaeothea, pycnogonid arthropods from the Lower Devonian Hunsrück Slate, West Germany.Palaeont. Z. 54:7–54.Google Scholar
  7. Blum, M.S. 1981. Chemical Defenses of Arthropods. Academic Press, New York.Google Scholar
  8. Bückmann, D., andTomaschko, K.-H. 1992. 20-Hydroxyecdysone stimulates molting in pycnogonid larvae (Arthropoda, Pantopoda).Gen. Comp. Endocrinol. 88:261–266.PubMedGoogle Scholar
  9. Bückmann, D., Starnecker, G., Tomaschko, K.-H., Wilhelm, E., Lafont, R., andGirault, J.P. 1986. Isolation and identification of major ecdysteroids from the pycnogonidPycnogonum litorale Ström (Arthropoda, Pantopoda).J. Comp. Physiol. B, 156:759–765.Google Scholar
  10. Connat, J.-L., Dotson, E.M., andDiehl, P.A. 1987. Metabolism of ecdysteroids in the female tickAmblyomma hebraeum (Ixodoidea, Ixodidae): accumulation of free ecdysone and 20-hydroxyecdysone in the eggs.J. Comp. Physiol. B. 157:689–699.PubMedGoogle Scholar
  11. Crothers, J.H. 1968. The biology of the shore crabCarcinus maenas (L.) 2. The life of the adult crab.Field Stud. 2:579–614.Google Scholar
  12. Dettner, K. 1987. Chemosystematics and evolution of beetle chemical defenses.Annu. Rev. Entomol. 32:17–48.Google Scholar
  13. Dettner, K. 1993. Defensive secretions and exocrine glands in free-living staphylinid beetles-their bearing on phylogeny (Coleoptera: Staphylinidae).Biochem. Syst. Ecol. 21:143–162.Google Scholar
  14. Garcia, M., Girault, J.-P., andLafont, R. 1986. Ecdysteroid metabolism in the terrestrial snailCepaea nemoralis (L.).Int. J. Invertebr. Reprod. Dev. 9:43–58.Google Scholar
  15. Gerhart, D.J., Bondura, M.E., andCommito, J.A. 1991. Inhibition of sunfish feeding by defensive steroids from aquatic beetles: Structure-activity relationships.J. Chem. Ecol. 17:1363–1370.Google Scholar
  16. Guerriero, A., andPietra, F. 1985. Isolation, in large amounts, of the rare plant ecdysteroid ajugasterone-C from the mediterranean zoanthidGerardia savaglia.Comp. Biochem. Physiol. B 80:277–278.Google Scholar
  17. Hashimoto, Y., andKonosu, S. 1978. Venoms of Crustacea and Merostomata, pp. 13–39,in S. Bettini (ed.). Arthropod Venoms. Springer, Berlin.Google Scholar
  18. Kittredge, J.S., andTakahashi, F.T. 1972. The evolution of sex pheromone communication in the arthropoda.J. Theor. Biol. 35:467–471.PubMedGoogle Scholar
  19. Koolman, J. 1990. Ecdysteroids.Zool. Sci. 7:563–580.Google Scholar
  20. Lafont, R., andHorn, D.H.S. 1989. Phytoecdysteroids: structures and occurrence, pp. 39–64,in J. Koolman (ed.). Ecdysone, From Chemistry to Mode of Action. Thieme, Stuttgart.Google Scholar
  21. Lafont, R., Bouthier, A., andWilson, I.D. 1991. Phytoecdysteroids: Structures, occurrence, biosynthesis and possible ecological significance, pp. 197–214,in I. Hrdy (ed.). Insect Chemical Ecology. SPB Academic Publishers, The Hague.Google Scholar
  22. Maróy, P., Kaufmann, G., andDübendorfer, A. 1988. Embryonic ecdysteroids ofDrosophila melanogaster.J. Insect Physiol. 34:633–637.Google Scholar
  23. Pasteels, J.M. 1993. The value of defensive compounds as taxonomic characters in the classification of leaf beetles.Biochem. Syst. Ecol. 21:135–142.Google Scholar
  24. Pasteels, J.M., andGrégoire, J.-C. 1983. The chemical ecology of defense in arthropods.Annu. Rev. Entomol. 28:263–289.Google Scholar
  25. Savolainen, V., Wuest, J., Lafont, R., andConnat, J.-L. 1991. Induction of supermolting in female ticksOrnithodoros moubata by ingestion of 20-hydroxyecdysone-22-acetate.Gen. Comp. Endocrinol. 82:221.Google Scholar
  26. Seifert, P. 1982. Studies on the sex pheromone of the shore crab,Carcinus maenas, with special regard to ecdysone excretion.Ophelia 21:147–158.Google Scholar
  27. Spencer, M., andCase, J.F. 1984. Exogenous ecdysteroids elicit low-threshold sensory responses in spiny lobsters.J. Exp. Zool. 229:163–166.Google Scholar
  28. Sturaro, A., Guerriero, A., De Clauser, R., andPietra, F. 1982. A new, unexpected marine source of a molting hormone. Isolation of ecdysterone in large amounts from the zoanthidGerardia savaglia.Experientia 38:1184–1185.Google Scholar
  29. Takahashi, F.T., andKittredge, J.S. 1973. Suppression of the feeding response in the crabPachygrapsus crassipes: Pheromone induction.Tex. Rep. Biol. Med. 31:403–408.Google Scholar
  30. Tomaschko, K.-H., andBückmann, D. 1990. Metabolism and excretion of ecdysteroids inPycnogonum litorale (Arthropoda, Pantopoda).Invert. Reprod. Dev. 18:130–131.Google Scholar
  31. Tomaschko, K.-H., andBückmann, D. 1993. Excessive abundance and dynamics of unusual ecdysteroids inPycnogonum litorale Ström (Arthropoda, Pantopoda).Gen. Comp. Endocrinol. 90:296–305.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • K-H Tomaschko
    • 1
  1. 1.Abteilung Allgemeine ZoologieUniversität UlmUlmGermany

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