Abstract
Leaf rinses ofS. berthaultii PI 473334 with methylene chloride were deterrent to feeding by the Colorado potato beetle when applied toS. tuberosum tuber and leaf disks. When the leaf rinse was separated into its nonvolatile and volatile fractions and applied to tuber disks, the nonvolatile fraction was highly deterrent, while the volatile fraction reduced consumption, but not significantly compared to the controls. A hexane leaf rinse was not deterrent to feeding, while an acetone rinse was approximately twofold more deterrent than the methylene chloride rinse when applied to leaf disks. Three cycles of bioassay-guided, reversed-phase open-column fractionation of an acetone leaf rinse yielded a relatively polar fraction with low deterrent activity, and two nonpolar fractions exhibiting higher specific activity. Reversed-phase preparative HPLC of these fractions yielded seven active fractions among the 10 assayed. Subsequent analytical HPLC indicated that two fractions each contained a single UV-absorbing compound, while another represented a mixture of at least four compounds. The remaining fractions were composed of complex mixtures of possibly ionic or polymeric compounds that were poorly resolved by HPLC.
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Avé, D.A., Gregory, P., andTingey, W.M. 1987. Aphid repellent sesquiterpenes in glandular trichomes ofSolanum berthaultii andS. tuberosum.Entomol. Exp. Appl. 44:131–138.
Casagrande, R.A. 1982. Colorado potato beetle resistance in a wild potato,Solanum berthaultii.J. Econ. Entomol. 75:368–372.
Chapman, R.F., andBernays, E.A. 1989. Insect behavior at the leaf surface and learning as aspects of host plant selection.Experientia 45:215–222.
Dethier, V.G. 1982. Mechanisms of host plant recognition.Entomol. Exp. Appl. 31:49–56.
Dimock, M.B., andTingey, W.M. 1988. Host acceptance behavior of Colorado potato beetle larvae influenced by potato glandular trichomes.Physiol. Entomol. 13:399–406.
Duffey, S.S., andFelton, G.W. 1991. Enzymatic antinutritive defenses of the tomato plant against insects, pp. 166–197,in P.A. Hedin (ed.). Naturally Occurring Pest Bioregulators. ACS Symposium Series 449. American Chemical Society, Washington, D.C. 456 pp.
Flanders, K.L., andRadcliffe, E.B. 1992. Host plant resistance inSolanum germplasm: an appraisal of resistance to the Colorado potato beetle, potato leafhopper and potato flea beetle. Minnesota Agric. Exp. Stn. Bull. No. 599. 22 pp.
Gibson, R.W. 1971. Glandular hairs providing resistance to aphids in certain wild potato species.Ann. Appl. Biol. 68:113–119.
Gregory, P., Avé, D.A., Bouthyette, P.Y., andTingey, W.M. 1986. Insect-defensive chemistry of potato glandular trichomes, pp. 173–183,in B.E. Juniper and T.R.E. Southwood (eds.). The Plant Surface and Insects. Edward Arnold Publishers, London. 360 pp.
Juniper, B.E., andSouthwood, T.R.E. (eds.). 1986. The Plant Surface and Insects. Edward Arnold Publishers, London. 360 pp.
King, R.R., Singh, R.P., andCalhoun, L.A. 1987. Isolation and characterization of 3,3′,4,6-tetra-O-acylated sucrose esters from the type B glandular trichomes ofSolanum berthaultii Hawkes (PI 265857).Carbohydr. Res. 172:113–121.
Kowalski, S.P., Eannetta, N.T., Hirzel, A.T., andSteffens, J.C. 1992. Purification and characterization of polyphenol oxidase from glandular trichomes ofSolanum berthaultii.Plant Physiol. 100:677–684.
Lapointe, S.L., andTingey, W.M. 1984. Feeding response of the green peach aphid (Homoptera: Aphididae) to potato glandular trichomes.J. Econ. Entomol. 77:386–389.
Miller, J.R., andStrickler, K.L. 1984. Finding and accepting host plants, pp. 127–157,in W.J. Bell and R.T. Cardé (eds.). Chemical Ecology of Insects. Sinauer Associates, Sunderland, Massachusetts. 524 pp.
Neal, J.J., Steffens, J.C., andTingey, W.M. 1989. Glandular trichomes ofSolanum berthaultii and resistance to the Colorado potato beetle.Entomol. Exp. Appl. 51:133–140.
Neal, J.J., Tingey, W.M., andSteffens, J.C. 1990. Sucrose esters of fatty acids in glandular trichomes ofSolanum berthaultii deter settling and probing by the green peach aphid.J. Chem. Ecol. 16:487–497.
Pelletier, Y., andSmilowitz, Z. 1990. Effect of type B exudate ofSolanum berthaultii Hawkes on consumption by the Colorado potato beetle,Leptinotarsa decemlineata (Say).J. Chem. Ecol. 16:1547–1555.
Plaisted, R.L., Tingey, W.M., andSteffens, J.C. 1992. The germplasm release of NYL 235-4, a clone with resistance to the Colorado potato beetle.Am. Potato J. 69:843–846.
Slansky, F., Jr., andRodriguez, J.G. 1987. Nutritional ecology of insects, mites, spiders, and related invertebrates: an overview, pp. 1–69,in F. Slansky Jr., and J.G. Rodriguez (eds.). Nutritional Ecology of Insects, Mites, Spiders, and Related Invertebrates. John Wiley & Sons, New York. 1016 pp.
Steffens, J.C., andWalters, D.S. 1991. Biochemical aspects of glandular trichome-mediated insect resistance in theSolanaceae, pp. 136–149,in P.A. Hedin (ed.). Naturally Occurring Pest Bioregulators. ACS Symposium Series 449. American Chemical Society, Washington, D.C. 456 pp.
Tingey, W.M. 1991. Potato glandular trichomes: defensive activity against insect attack, pp. 126–135,in P.A. Hedin (ed.). Naturally Occurring Pest Bioregulators. ACS Symposium Series 449. American Chemical Society, Washington, D.C. 456 pp.
Tingey, W.M., andGibson, R.W. 1978. Feeding and mobility of the potato leafhopper impaired by glandular trichomes ofSolanum berthaultii andS. polyadenium.J. Econ. Entomol. 71:856–858.
Tingey, W.M., andLaubengayer, J.E. 1986. Glandular trichomes of a resistant hybrid potato alter feeding behavior of the potato leafhopper (Homoptera: Cicadellidae).J. Econ. Entomol. 79:1230–1234.
Tingey, W.M., Plaisted, R.L., Laubengayer, J.E., andMehlenbacher, S.A. 1982. Green peach aphid resistance by glandular trichomes inSolanum tuberosum ×Solanum berthaultii hybrids.Am. Potato J. 59:241–251.
Yencho, G.C., andTingey, W.M. 1994. Glandular trichomes ofSolanum berthaultii alter host preference of the Colorado potato beetle,Leptinotarsa decemlineata. Entomol. Exp. Appl. In press.
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Yencho, G.C., Renwick, J.A.A., Steffens, J.C. et al. Leaf surface extracts ofSolanum berthaultii hawkes deter colorado potato beetle feeding. J Chem Ecol 20, 991–1007 (1994). https://doi.org/10.1007/BF02059737
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DOI: https://doi.org/10.1007/BF02059737