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Autoradiographische Untersuchungen über die Zellkinetik der enchondralen Ossifikation der Maus nach Oestrogen- und Testosteronverabreichung

  • Published:
Zeitschrift für die gesamte experimentelle Medizin

Zusammenfassung

  1. 1.

    Zur Prüfung der Oestrogen- und Testosteroneinwirkung auf die enchondrale Ossifikation erhalten 25 g schwere, 5 Monate alte, männliche Mäuse täglich intraperitoneal je 2 mg Oestrogen bzw. Testosteron. Die Mäuse werden 7, 13, 20 und 27 Tage nach Versuchsbeginn getötet, nachdem ihnen zusätzlich 40 min vor dem Tode intraperitoneal eine Dosis von 25ΜC3H-Thymidin injiziert wurde.

  2. 2.

    Die Knochenneubildung an der proximalen Tibiametaphyse ist bei den Testosteronmäusen gegenüber den Kontrollen nicht gesteigert, sogar eher gehemmt. Die Oestrogenmäuse zeigen eine stark gesteigerte enchondrale, endostale und periostale Knochenneubildung. Der prozentuale Anteil des Knochengewebes zum Gesamtgewicht der Metaphyse beträgt bei den Kontrollmäusen 28,6%, bei den Testosteronmäusen 24,6% und bei den Oestrogenmäusen 39,6%.

  3. 3.

    Der3H-Index der Präosteoblasten der Testosteronmäuse liegt während der ganzen Versuchsdauer im Bereich der Norm (7%). Der3H-Index der Präosteoblasten steigt bei den Oestrogenmäusen innerhalb von 7 Tagen von 7 auf 16% an und stabilisiert sich im weiteren Versuchs-verlauf bei 33%. Nach den autoradiographischen Befunden besteht die Oestrogenwirkung sowohl in einer internen Anregung der Neubildung von Präosteoblasten als auch in einer Beschleunigung der Ausdifferenzierung der Präosteoblasten in Osteoblasten.

Summary

  1. 1.

    To examine the effects of sex hormones on endochondral ossification, 5–6 month old male „Swiss Mice“, each weighing approximately 25 grams, received daily 2 mg intraperitoneal doses of estrogen or testosterone. The mice were killed 7, 13, 20 and 27 days after the start of the experiment. In addition, 25ΜC3H-thymidine was administered intraperitoneally to each mouse 40 minutes before death.

  2. 2.

    There is no increased bone formation in the proximal tibial metaphysis of the testosterone mice compared with control mice. In fact, new bone formation in the testosterone mice appears to be inhibited. The estrogen mice exhibit an exceptional increase in endochondral, endostal and periostal new bone formation. A weight comparison of the component bone tissue to the total tibial metaphysis yields a percentile of 28,6% for the control mice, 24,6% for the mice treated with testosterone, and 39,6% for those treated with estrogen.

  3. 3.

    The3H-Index of the preosteoblasts remains uniformly constant at the normal value of 7% in all the mice treated with testosterone. Estrogen increases the3H-Index of the preosteoblasts from 7% to 16% after only 7 days of treatment, and then stabilizes at 33% for the duration of the experiment. According to these autoradiographic findings, estrogen apparently internally stimulates preosteoblastic formation, and also accelerates differentiation of preosteoblasts into osteoblasts.

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  1. Pathologischen Institut der Universität Zürich, Schweiz

    Franklin Holzer

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Holzer, F. Autoradiographische Untersuchungen über die Zellkinetik der enchondralen Ossifikation der Maus nach Oestrogen- und Testosteronverabreichung. Z. Gesamte Exp. Med. 139, 213–226 (1965). https://doi.org/10.1007/BF02059620

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