Advertisement

Calcified Tissue Research

, Volume 15, Issue 1, pp 315–324 | Cite as

Microinterferometry of developing bone mineral

  • W. L. Past
Original Papers

Abstract

A method based on tetracycline labeling was developed for selecting, in frozen sections of fetal mouse femur, bone foci approximately 6, 24, 48 and 72 h of age. Microinterferometric measurements of these foci permitted a calculation of the effective thickness (t) and refractive index (n) of each focus. After demineralizing the sections by a method which left the organic portion of the bone intact, the foci were re-measured andt andn of the organic compartment of each focus were determined;t andn of the mineral compartment were calculated by difference. A sharp decrease and subsequent rise in then of whole bone occurred between 6 and 48 h. These changes derived from the mineral compartment, and were thought to have resulted from the formation of amorphous calcium phosphate and hydroxyapatite, respectively. The mineral present in 6 h foci, however, was believed to be some precursor of amorphous calcium phosphate.

Key words

Bone Mineral Osteogenesis Interference microscopy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andrews, C. L.: Optics of the electromagnetic spectrum, p. 345. Englewood Cliffs, N.J.: Prentice-Hall, Inc. 1960.Google Scholar
  2. Ascenzi, A., Fabry, C.: Technique for dissection and measurement of refractive index of osteones. J. biophys. biochem. Cytol.6, 139–143 (1959)PubMedGoogle Scholar
  3. Barer, R., Joseph, S.: Refractometry of living cells. Part I. Basic principles. Quart. J. micr. Sci.95, 399–423 (1954)Google Scholar
  4. Bills, C. E., Eisenberg, H., Pallante, S. L.: Complexes of organic acids with calcium phosphate: the von Kossa stain as a clue to the composition of bone mineral. Johns Hopk. med. J.128, 194–207 (1971)Google Scholar
  5. Bonucci, E.: The locus of initial calcification in cartilage and bone. Clin. Orthop. Rel. Res.78, 108–139 (1971)Google Scholar
  6. Brown, W. E.: Crystal growth of bone mineral. Clin. Orthop.44, 205–220 (1966)PubMedGoogle Scholar
  7. Brown, W. E., Smith, J. P., Lehr, J. R., Frazier, A. W.: Crystallographic and chemical relations between octacalcium phosphate and hydroxyapatite. Nature (Lond.)196, 1050–1055 (1962).Google Scholar
  8. Davies, H. G., Engström, A.: Interferometric and X-ray absorption studies of bone tissue. Exp. Cell Res.7, 243–255 (1954).PubMedGoogle Scholar
  9. Eanes, E. D., Termine, J. D., Nylen, M. U.: An electron microscopic study of the formation of amorphous calcium phosphate and its transformation to crystalline apatite. Calcif. Tiss. Res.12, 143–158 (1973)Google Scholar
  10. Goldstein, D. J.: Relation of effective thickness and refractive index to permeability of tissue components in fixed sections. J. roy. micr. Soc.84, 43–54 (1964)Google Scholar
  11. Harper, R. A., Posner, A. S.: Measurement of noncrystalline calcium phosphate in bone mineral. Proc. Soc. exp. Biol. (N.Y.)122, 137–142 (1966)Google Scholar
  12. Holmes, J. M., Beebe, R. A.: Surface areas by gas adsorption on amorphous calcium phosphate and crystalline hydroxyapatite. Calcif. Tiss. Res.7, 163–174 (1971)Google Scholar
  13. Kashiwa, H. K., House, Jr., C. M.: The glyoxal bis (2-hydroxy-anil) method modified for localizing insoluble calcium salts. Stain Technol.39, 359–367 (1964)PubMedGoogle Scholar
  14. Larsen, E. S., Berman, H.: The microscopic determination of the nonopaque minerals, p. 84, 111, 104, second ed. Washington: U. S. Government Printing Office 1934Google Scholar
  15. Molnar, Z.: Development of the parietal bone of young mice: 1. Crystals of bone mineral in frozen-dried preparations. J. Ultrastruct. Res.3, 39–45 (1959)PubMedGoogle Scholar
  16. Neuman, W. F., Neuman, M. W.: The chemical dynamics of bone mineral, p. 55–64. Chicago: Chicago University Press 1958Google Scholar
  17. Past, W. L., Schrodt, G. R.: A microscopic method for determining bone crystal solubility. Calcif. Tiss. Res.4, 48–59 (1969)Google Scholar
  18. Posner, A. S.: Bone mineral on the molecular level. Fed. Proc.32, 1933–1937 (1973)PubMedGoogle Scholar
  19. Robinson, R. A., Watson, M. L.: Crystal-collagen relationships in bone as observed in the electron microscope. III. Crystal and collagen morphology as a function of age. Ann. N.Y. Acad. Sci.60, 595–628 (1955)Google Scholar
  20. Ross, K. F. A.: Phase contrast and interference microscopy for cell biologists, p. 128–134. London: Edward Arnold Ltd. 1967Google Scholar
  21. Scott, J. D.: Handbook of chemistry and physics, p. 554, forty-first ed. Cleveland: Chemical Rubber Publishing Co. 1959Google Scholar
  22. Termine, J. D., Eanes, E. D.: Comparative chemistry of amorphous and apatitic calcium phosphate preparations. Calcif. Tiss. Res.10, 171–197 (1972)Google Scholar
  23. Termine, J. D., Posner, A. S.: Infrared determination of the percentage of crystallinity in apatitic calcium phosphates. Nature (Lond.)211, 268–270 (1969)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • W. L. Past
    • 1
  1. 1.Department of PathologyUniversity of Louisville School of MedicineLouisvilleUSA

Personalised recommendations