Summary
The change in the nuclear size of neonatal rat myocardial cells was evaluated under the culture conditions of exposure to norepinephrine (NE). Daily administration for 1 week of 0.2, 2, and 20 ng/ml NE induced a significant increase in nuclear size as a result of the dose-dependent quality of the nuclei. NE also stimulated a beating response in the cultured myocardial cells because of this dose dependency. A good correlation was found between the two markers and the NE dose dependency. Single or mononucleated myocardial cells often appeared in the NE-treated groups. Ornithine decarboxylase activity was not acutely stimulated even by 20 ng/ml NE. These observations suggest that administration of NE induces nuclear enlargement and enhances nuclear function through the stimulation of beating. Further, there may not be a direct relation between nuclear enlargement and the polyamine synthesis pathways.
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References
Yamori Y, Nakada T, Lovenberg W (1976) Effect of antihypertensive therapy on lysine incorporation into vascular protein of the spontaneously hypertensive rat. Eur J Pharmacol 38: 349–355
Yamori Y (1974) Contribution of cardiovascular factors to the development of hypertension in spontaneously hypertensive rats. Jpn Heart J 15: 194–196
Yamori Y, Tarazi RC, Ooshima A (1980) Effect ofβ-receptor blocking agents on cardiovascular structure changes in spontaneous and noradrenaline-induced hypertension in rats. Clin Sci 59: 457s–460s
Nagatsu T, Kato T, Hashimoto Y, Yamori Y, Okamoto K (1974) Dopamine-β-hydroxylase activity in stroke-prone spontaneously hypertensive rats. Experientia 34: 305–306
Simpson P, McGrath P, Savion S (1982) Myoyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines. Circ Res 51: 787–801
Kaneko H, Goshima K (1982) Selective killing of fibroblast-like cells in cultures of mouse heart cells by treatment with a calcium ionophore, A-23187. Exp Cell Res 142:407–416
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Stevens L (1970) The biochemical role of naturally occurring polyamines in nucleic acid synthesis. Biol Rev 45: 1–27
Russel DH, Shverick KT, Hamrell BB, Alpert NR (1981) Polyamine synthesis during initial phase of stress-induced cardiac hypertrophy. Am J Physiol 221: 1287–1291
Bartolome J, Huguenard J, Slotkin TA (1980) Role of ornithine decarboxylase in cardiac growth and hypertrophy. Science 210: 793–794
Caldarera CM, Casti A, Rossoni C, Visioli O (1971) Polyamines and noradrenaline following myocardial hypertrophy. J Mol Cell Cardiol 3: 121–126
Limas CJ, Limas C (1978) Cardiac DNA polymerases during postnatal development. Nature 271: 781–783
Schwartz SM, Gordon R, Campbell R, Campbell JM (1986) Replication of smooth muscle cells in vascular disease. Circ Res 58: (4) 427–444
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Igawa, T., Yamori, Y., Lewis, L.J. et al. Norepinephrine-induced enlargement of nucleus in cultured myocardial cells. Heart Vessels 4, 1–5 (1988). https://doi.org/10.1007/BF02058680
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DOI: https://doi.org/10.1007/BF02058680