Mycopathologia et mycologia applicata

, Volume 6, Issue 4, pp 317–324 | Cite as

Immunologic studies on the etiologic agents of North and South American blastomycosis

II. Comparison of serologic reactions
  • Lorraine Friedman
  • Norman F. Conant


This study presents an immunologic comparison of the fungi,B. brasiliensis andB. dermatitidis, by serologic reactions in animals and in humans.

The complement fixation test was used to test the sera of rabbits with various homologous and heterologous antigens of the two fungi: yeast-phase, broth filtrates of the mycelial phase, and “purified” protein. A definite cross-reactivity was observed.

Sera from humans infected withB. dermatitidis orB. brasiliensis also were tested by complement fixation reaction with homologous and heterologous antigens: yeast-phase, broth filtrates of the mycleial phase, and “purified” protein. Two of nine sera from cases of North American blastomycosis, giving positive reactions with the homologous antigens, cross-reacted at a low titer with the heterologous antigens. Five of eleven sera, positive withB. brasiliensis antigens, reacted with one or more types ofB. dermatitidis antigens. There was no correlation between titer obtained with the homologous antigens and that obtained with the heterologous antigens.

Immunization of rabbits was best achieved by use of massive doses of sonic-vibrated yeast-phase cell suspensions.

Data were presented indicating the greater fragility ofB. brasiliensis yeast-phase cells as compared withB. dermatitidis cells of the same phase. Evidence was obtained by subjecting cells to sonic vibrations.


Etiologic Agent Immunologic Study Complement Fixation Complement Fixation Test Blastomycosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Friedman, L. andConant, N. F. Immunologic studies on the etiologic agents of North and South American blastomycosis. I. Comparison of hypersensitivity reactions.Google Scholar
  2. 2.
    Smith, D. T. 1949 Immunologic types of blastomycosis: A report of 40 cases. Annals of Int. Med.,31, 463–469.Google Scholar
  3. 3.
    Martin, D. S. andSmith, D. T. 1936 The laboratory diagnosis of blastomycosis. J. Lab. & Clin. Med.,21, 1289–1296.Google Scholar
  4. 4.
    Lacaz, C. S. 1949 Novos dados en relacãõ à blastomicose sul-americana e seu agent etiologico. Rev. Med. e. cir. de Sãõ Paulo,9, 303–341.Google Scholar
  5. 5.
    Saslaw, S. andCampbell, C. C. 1948 The use of yeast-phase antigens in a complement fixation test for histoplasmosis. I. Preliminary results with rabbit sera. J. Lab. & Clin. Med.,33, 811–818.Google Scholar
  6. 6.
    Campbell C. C. andSaslaw, S. 1949 Use of yeat-phase antigens in a complement fixation test for histoplasmosis. III. Preliminary results with human sera. Pub. Health Rep.,64, 551–560.Google Scholar
  7. 7.
    Kent, J. F. 1946 An abbreviated spectrophotometric technique for determining the optimal concentration of amboceptor., J. Lab. & Clin. Med.,31, 1270.Google Scholar
  8. 8.
    Kent, J. F. Personal Communication.Google Scholar
  9. 9.
    Kent, J. F., Bukantz, S. C., andRein, C. R. 1946 Studies in complement fixation. I. Spectrophotometric titration of complement: constuction of graphs for direct determination of the 50 per cent hemolytic unit. J. Immunol.,53, 37.Google Scholar
  10. 10.
    Bukantz, S. C., Rein, C. R., andKent, J. F. 1946 Studies in complement fixation. II. Preservation of sheep's blood in citrate dextrose mixtures (modified Alsever's solution) for use in complement fixation reaction. J. Lab. & Clin. Med.,31, 394.Google Scholar
  11. 11.
    Kent, J. F. 1950 The application of quantitative methods in studies of the antigen-antibody reactions, thesis, Duke University.Google Scholar
  12. 12.
    Martin, D. S. 1950 Some epidemiologic and immunologic studies on North American blastomycosis, thesis, Columbia University.Google Scholar
  13. 13.
    Bendich, A. andChargraff, E. 1946 The isolation and characterization of two antigenic fractions of Broteus OX-19. J. Biol. Chem.,166, 283.Google Scholar
  14. 14.
    Mackinnon, J. E. andVinelli, H. 1949 Observaciones sobre la forma multibrotante de Paracoccidioides brasiliensis en los cultivos. An. Fac. de med. de Montevideo,34, 461.Google Scholar

Copyright information

© Uitgeverij Dr. W. Junk 1953

Authors and Affiliations

  • Lorraine Friedman
    • 1
  • Norman F. Conant
    • 1
  1. 1.Department of BacteriologyDuke University School of MedicineDurhamUSA

Personalised recommendations