Immunologic studies on the etiologic agents of North and South American blastomycosis
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This study presents an immunologic comparison of the fungi,B. brasiliensis andB. dermatitidis, by serologic reactions in animals and in humans.
The complement fixation test was used to test the sera of rabbits with various homologous and heterologous antigens of the two fungi: yeast-phase, broth filtrates of the mycelial phase, and “purified” protein. A definite cross-reactivity was observed.
Sera from humans infected withB. dermatitidis orB. brasiliensis also were tested by complement fixation reaction with homologous and heterologous antigens: yeast-phase, broth filtrates of the mycleial phase, and “purified” protein. Two of nine sera from cases of North American blastomycosis, giving positive reactions with the homologous antigens, cross-reacted at a low titer with the heterologous antigens. Five of eleven sera, positive withB. brasiliensis antigens, reacted with one or more types ofB. dermatitidis antigens. There was no correlation between titer obtained with the homologous antigens and that obtained with the heterologous antigens.
Immunization of rabbits was best achieved by use of massive doses of sonic-vibrated yeast-phase cell suspensions.
Data were presented indicating the greater fragility ofB. brasiliensis yeast-phase cells as compared withB. dermatitidis cells of the same phase. Evidence was obtained by subjecting cells to sonic vibrations.
KeywordsEtiologic Agent Immunologic Study Complement Fixation Complement Fixation Test Blastomycosis
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