Protoplasma

, Volume 79, Issue 1–2, pp 127–143 | Cite as

Changes in nucleolar activity during the growth and development of the wheat coleoptile

  • R. J. Rose
Article

Summary

In the dormant embryonic wheat coleoptile the nucleolus is almost a purely fibrillar structure and numerous large blocks of heterochromatin are present in the nucleus. Following germination the nucleoli develop distinct granular and fibrillar zones which become intricately intermingled, and the nuclear heterochromatin disperses. What appear to be perichromatin granules have been noted in coleoptile nuclei. When these dark grown coleoptiles cease division growth the nucleoli once again assume a largely fibrillar structure, and show low incorporation of3H-uridine. Associated with these latter changes at the cessation of meristematic growth there is a dilution of cytoplasmic ribosomes. The cytoplasm up until this time is densely packed with ribosomes. This study, taken together with other reports, suggests that characteristic nucleolar structural changes and reduced nucleolar activity are often associated with the differentiation of plant cells, following the cessation of meristematic growth.

Keywords

Plant Cell Structural Change Large Block Fibrillar Structure Wheat Coleoptile 

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References

  1. Avery, G. S., andP. R. Burkholder, 1936: Polarised growth and cell studies on theAvena coleoptile, phytohormone test object. Bull. Torrey Bot. Club63, 1–15.Google Scholar
  2. Barlow, P. W., 1970: RNA synthesis in the root apex ofZea mays. J. Exp. Bot.21, 292–299.Google Scholar
  3. Brown, D. D., 1967: The genes for ribosomal RNA and their transcription during amphibian development. In: Current topics in developmental biology. Volume 2 (A. A. Moscona, andA. Monroy, eds.), New York: Academic Press.Google Scholar
  4. Chouinard, L. A., 1966: Nucleolar architecture in root meristematic cells ofAllium cepa. Nat. Cancer Inst. Monogr.23, 125–143.PubMedGoogle Scholar
  5. —, 1970: Localisation of intranucleolar DNA in root meristematic cells ofAllium cepa. J. Cell Sci.6, 73–85.PubMedGoogle Scholar
  6. Deltour, R., etR. Bronchart, 1971: Changements de l'ultrastructure des cellules radiculaires deZea mays au debut de la germination. Planta (Berl.)97, 197–207.Google Scholar
  7. Esau, K., 1972: Changes in the nucleus and the endoplasmic reticulum during differentiation of a sieve element inMimosa pudica L. Ann. Bot.36, 703–710.Google Scholar
  8. —, andR. H. Gill, 1972: Nucleus and endoplasmic reticulum in differentiating root protophloem ofNicotiana tabacum. J. Ultrastruct. Res.41, 160–175.PubMedGoogle Scholar
  9. Fowke, L. C., andG. Setterfield, 1968: Cytological responses in Jerusalem artichoke tuber slices during ageing and subsequent auxin treatment. In: Biochemistry and physiology of plant growth substances (F. W. Wightman, andG. Setterfield, eds.). Ottawa: The Runge Press.Google Scholar
  10. Franke, W. W., M. F. Trendelenburg, andU. Scheer, 1973: Natural segregation of nucleolar components in the course of a plant cell differentiation. Planta (Berl.)110, 159–164.Google Scholar
  11. Frenster, J. H., 1969: Biochemistry and molecular biophysics of heterochromatin and euchromatin. In: Handbook of molecular cytology (A. Lima-de-faria, ed.). Amsterdam-London: North-Holland Publishing Company.Google Scholar
  12. Hallam, N. D., B. E. Roberts, andD. J. Osborne, 1972: Embryogenesis and germination in rye (Secale cereale L.). II. Biochemical and fine structural changes during germination. Planta (Berl.)105, 293–309.Google Scholar
  13. Hay, E. D., 1968: Structure and function of the nucleolus in developing cells. In: The nucleus (A. J. Dalton, andF. Haguenau, eds.). New York: Academic Press.Google Scholar
  14. Hyde, B. B., 1967: Changes in nucleolar ultrastructure associated with differentiation in the root tip. J. Ultrastruct. Res.18, 25–54.PubMedGoogle Scholar
  15. Jones, K. W., 1965: The role of the nucleolus in the formation of ribosomes. J. Ultrastruct. Res.13, 257–262.PubMedGoogle Scholar
  16. Jordan, E. G., andJ. M. Chapman, 1971: Ultrastructural changes in the nucleoli of Jerusalem artichoke (Heliantus tuberosus) tuber discs. J. Exp. Bot.22, 627–634.Google Scholar
  17. — —, 1973: Nucleolar and nuclear envelope ultrastructure in relation to cell activity in discs of carrot root (Daucus carota L.). J. Exp. Bot.24, 197–209.Google Scholar
  18. King, B., andJ. M. Chapman, 1972: The effect of inhibitors of protein and nucleic acid synthesis on nucleolar size and enzyme induction in Jerusalem artichoke tuber slices. Planta (Berl.)104, 306–315.Google Scholar
  19. Leduc, E. H., andW. Bernhard, 1962: Water soluble embedding media for ultrastructure cytochemistry. Digestion with nucleases and proteinases. In: The interpretation of ultrastructure (R. J. C. Harris, ed.). New York: Academic Press.Google Scholar
  20. Littau, V. C., V. G. Allfrey, J. H. Frenster, andA. E. Mirsky, 1964: Active and inactive regions of nuclear chromatin as revealed by electron microscope autoradiography. Proc. nat. Acad. Sci.52, 93–100.PubMedGoogle Scholar
  21. Lord, A., andJ. G. Lafontaine, 1969: The organisation of the nucleolus in meristematic plant cells. A cytochemical study. J. Cell Biol.40, 633–647.PubMedGoogle Scholar
  22. Masuda, Y., G. Setterfield, andS. T. Bayley, 1966: Ribonucleic acid metabolism and cell expansion in oat coleoptile. Plant and Cell Physiol.7, 243–262.Google Scholar
  23. Monneron, A., andW. Bernhard, 1969: Fine structural organization of the interphase nucleus in some mammalian cells. J. Ultrastruct. Res.27, 266–288.PubMedGoogle Scholar
  24. Nitsch, J. P., andC. Nitsch, 1956: Studies on the growth of coleoptile and first internode sections. A new, sensitive, straight growth test for auxins. Plant Physiol.31, 94–111.Google Scholar
  25. O'Brien, T. P., andK. V. Thimann, 1967: Observations on the fine structure of the oat coleoptile. II. The parenchyma cells of the apex. Protoplasma63, 417–442.Google Scholar
  26. Ray, P. M., T. L. Shininger, AndM. M. Ray, 1969: Isolation of ß-glucan synthetase particles from plant cells and indentification with golgi membranes. Proc. nat. Acad. Sci.64, 605–612.Google Scholar
  27. Rejman, E., andJ. Buchowicz, 1971: The sequence of initiation of RNA, DNA and protein synthesis in the wheat grains during germination. Phytochemistry10, 2951–2957.Google Scholar
  28. Rose, R. J., andD. Adamson, 1969: A sequential response to growth substances in coleoptiles from γ-irradiated wheat. Planta (Berl.)88, 274–281.Google Scholar
  29. —,L. C. Fowke, andG. Setterfield, 1972: Nucleolar activation and the function of nucleolar vacuoles in Jerusalem artichoke tuber slices. Exp. Cell Res.71, 1–16.PubMedGoogle Scholar
  30. —,J. Gregory, andF. V. Mercer, 1970: The effect of 5-fluorouracil on the growth and nucleolus of wheat coleoptiles. Aust. J. Biol. Sci.23, 561–571.Google Scholar
  31. —, andG. Setterfield, 1971: Cytological studies on the inhibition by 5-fluorouracil of ribosome synthesis and growth in Jerusalem artichoke tuber slices. Planta (Berl.)101, 210–230.Google Scholar
  32. Setterfield, G., 1961: Structure and composition of plant-cell organelles in relation to growth and development. Canad. J. Bot.39, 469–489.Google Scholar
  33. Steffensen, D. M., 1966: Synthesis of ribosomal RNA during growth and division inLilium. Exp. Cell Res.44, 1–12.PubMedGoogle Scholar
  34. Stevens, B. J., andH. Swift, 1966: RNA transport from nucleus to cytoplasm inChironomus salivary glands. J. Cell Biol.31, 55–57.PubMedGoogle Scholar
  35. Street, H. E., H.Öpik, and F. E. L.James: Fine structure of the main axis meristem of cultured tomato roots. Phytomorphology17, 391–401.Google Scholar
  36. Swift, J. G., andT. P. O'Brien, 1972 a: The fine structure of the wheat scutellum before germination. Aust. J. Biol. Sci.25, 9–22.Google Scholar
  37. — —, 1972 b: The fine structure of the wheat scutellum during germination. Aust. J. Biol. Sci.25, 469–486.Google Scholar
  38. Thomas, C., 1972: Correlation between ultrastructural aspect of nucleoli and inhibition of ribosomal RNA synthesis inXenopus laevis oocytes. Exp. Cell Res.74, 547–551.PubMedGoogle Scholar
  39. Van Gansen, P., andA. Schramm, 1972: Evolution of the nucleoli during oogenesis inXenopus laevis studied by electron microscopy. J. Cell Sci.10, 339–367.PubMedGoogle Scholar
  40. Wright, S. T. C., 1961: Growth and cellular differentiation in the wheat coleoptile (Triticum vulgare L.). 1. Estimation of cell number, cell volume and certain nitrogenous constituents. J. Exp. Bot.12, 303–318.Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • R. J. Rose
    • 1
  1. 1.Department of Botany and ZoologyMassey UniversityPalmerston NorthNew Zealand

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