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Immunoscintigraphy of colorectal cancer with an antibody to epithelial membrane antigen (EMA)

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  • Published:
Diseases of the Colon & Rectum

Abstract

Immunoperoxidase staining of LICR-LON M8, a mouse monoclonal antibody reactive with epithelial membrane antigen, showed a strong reaction with colorectal cancer. This finding prompted an immunoscintigraphic study of colorectal cancer patients using this antibody. Sixteen patients had external gamma scintigraphy after intravenous injection of indium 111-labeled M8. Positive scans were obtained in 11 of the 13 patients with primary colorectal cancers, and 2 of the 3 patients with recurrent tumors. The high indium 111 background in the liver prevented the detection of hepatic metastases in 5 patients. Twelve patients had samples taken of tumor, normal colon, and venous blood at the time of surgery. The ratio of labeled antibody uptake in tumor to that of blood was 5.1 (±3.6 S.D.), which was significantly different (P=0.001) to that of the similar ratio for normal colon (2.0±1.6 S.D.). The tumor to normal colon uptake ratio was 2.6 (±1.3 S.D.). These results suggest a specific uptake of indium 111-labeled M8 by colorectal cancer.

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Authors and Affiliations

  1. Department of Surgery, University College London, The Rayne Institute, 5 University Street, WC1E 6JJ, London, UK

    Chu-Yiu Yiu F.R.C.S., Louise A. Baker B.Sc., Brian R. Davidson F.R.C.S., Paul B. Boulos M.S., F.R.C.S. & Charles G. Clark M.S., F.R.C.S.

  2. Department of Nuclear Medicine, Royal Marsden Hospital, Sutton, Surrey, UK

    Michael Ward M.D., M.R.C.P., Ken Roberts Ph.D. & John Westwood Ph.D.

  3. Department of Nuclear Medicine, University College Hospital, London, UK

    Gill Clarke B.Sc.

  4. Department of Nuclear Medicine, St. George's Hospital, Tooting, London, UK

    Caroline Ward F.R.C.R.

Authors
  1. Chu-Yiu Yiu F.R.C.S.
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  2. Louise A. Baker B.Sc.
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  3. Brian R. Davidson F.R.C.S.
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  4. Michael Ward M.D., M.R.C.P.
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  5. Ken Roberts Ph.D.
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  6. Gill Clarke B.Sc.
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  7. Caroline Ward F.R.C.R.
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  8. John Westwood Ph.D.
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  9. Paul B. Boulos M.S., F.R.C.S.
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  10. Charles G. Clark M.S., F.R.C.S.
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Additional information

Read at the XIIth Biennial Congress of the International Society of University Colon and Rectal Surgeons, Glasgow, Scotland, July 10 to 14, 1988.

Mr. C. Y. Yiu was supported by a Wellcome Lectureship in Surgery.

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Yiu, CY., Baker, L.A., Davidson, B.R. et al. Immunoscintigraphy of colorectal cancer with an antibody to epithelial membrane antigen (EMA). Dis Colon Rectum 33, 122–126 (1990). https://doi.org/10.1007/BF02055540

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  • DOI: https://doi.org/10.1007/BF02055540

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