Summary
1. A chemically defined, minimal medium was developed for the cultivation ofCryptococcus nigricans (glucose, nitrate, thiamin, biotin, and inorganic salts).
2. Optimum temperature was found to be 29° C with upper and lower limits at 34° C and 23° C.
3. Optimum pH for growth was found to be pH 5.0–5.6.
4. Ammonium nitrogen was not assimilated.
5.C. nigricans was inhibited by Mycostatin, Amphotericin A and B, and Polymyxin B.
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This study was supported in part by a grant-in-aid from the Rutgers Research Council.
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Rich, M.A., Stern, A.M. Studies on cryptococcus nigricans, II. The effects of physical, chemical and nutritional factors. Mycopathologia et Mycologia Applicata 10, 83–90 (1958). https://doi.org/10.1007/BF02055035
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DOI: https://doi.org/10.1007/BF02055035