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Influence of misoprostol, a synthetic prostaglandin E1 analog, on the healing of colonic anastomoses in rats

  • Original Contributions
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Diseases of the Colon & Rectum

Abstract

PURPOSE: This study was designed to examine the influence of misoprostol, a synthetic prostaglandin E 1 analog, on the healing of colonic anastomoses in rats, with particular regard to changes in collagen levels at the site of the anastomoses and their histopathologic aspects. METHODS: Sixty rats were submitted to resection and anastomosis of the colon, and divided at random into two groups. The test group received misoprostol intragastrically (200 μg/kg body weight), twice daily, from the day of operation until sacrifice. Controls received 0.9 percent NaCl. The animals were sacrificed on the third, seventh, or fourteenth postoperative day, and the results of the histopathologic analyses and hydroxyproline concentrations were compared. RESULTS: Our results show that misoprostol administration increased the hydroxyproline concentration on the fourteenth postoperative day without interfering in the inflammatory response (P<0.05).CONCLUSION: Misoprostol interferes with the balance between the synthesis and degradation of collagen, resulting in an elevation of collagen levels by the fourteenth postoperative day without influencing the inflammatory response.

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Authors and Affiliations

  1. Department of Pathology, University of Sõ Paulo, Ribeirão Preto, Brazil

    Edson Garcia Soares M.D.

  2. Department of Surgery Orthopedics and Traumatology, Laboratory of Experimental Surgery, Faculty of Medicine of Ribeirão Preto, University of Sõ Paulo, Ribeirão Preto, Brazil

    Francisco Aprilli M.D.

  3. Departamento de Clínica Cirúrgica, Faculdade de Ciências da Saúde, Universidade de Brasília, 70.000, Brasilia, Distrito Federal, Brazil

    Paulo Gonçalves de Oliveira M.D.

Authors
  1. Paulo Gonçalves de Oliveira M.D.
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  2. Edson Garcia Soares M.D.
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  3. Francisco Aprilli M.D.
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de Oliveira, P.G., Soares, E.G. & Aprilli, F. Influence of misoprostol, a synthetic prostaglandin E1 analog, on the healing of colonic anastomoses in rats. Dis Colon Rectum 37, 660–663 (1994). https://doi.org/10.1007/BF02054408

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