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Radioimmunoassay of phenytoin

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Abstract

We describe a radioimmunoassay procedure for the measurement of phenytoin (5,5-diphenylhydantoin) in serum samples. Antiserum to phenytoin has been produced against phenytoin-valeric acid-bovine serum albumin conjugate.125I labelled phenytoin-acetic acid-tyrosine methyl ester has been used as a tracer. The assay covers a range of 10–500 ng/cm3 and has a sensitivity of 0.25 ng. The assay is validated by specificity tests, precision profile and recovery tests.

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References

  1. F. BUCHTHAL, O. SEVENSMARK, AMA Archives of Neurol., 2 (1960) 624.

    Google Scholar 

  2. W. H. REYNOLDS, D. CHADWICK, A. W. GALBRAITH, Lancet, 1 (1976) 923.

    Google Scholar 

  3. F. ROCHNER, W. D. HOOPER, J. H. TYRER, M. J. EADIE, J. Neurol. Neurosurg. Psych., 35 (1972) 873.

    Google Scholar 

  4. A. RICHENS, A. DUNLOP, Lancet, 2 (1975) 247.

    Google Scholar 

  5. J. W. PAXTON, F. J. ROWELL, J. G. RATCLIFFE, J. Immunol. Methods, 10 (1976) 317.

    Google Scholar 

  6. W. M. HUNTER, F. C. GREENWOOD, Nature (London), 194, (1962) 495.

    Google Scholar 

  7. C. E. COOK, J. A. KEPLER, H. D. CHRISTENSEN, Res. Com. Chem. Pathol. Pharmacol., 5 (1973) 767.

    Google Scholar 

  8. GRACE SAMUEL, N. SIVAPRASAD, R. S. MANI, Symp. on Radiochemistry and Radiation Chemistry, Preprints, IGCAR, Kalpakkam, India, 1989.

    Google Scholar 

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Authors and Affiliations

  1. Isotope Division, Bhabha Atomic Research Centre, Trombay, 400085, Bombay, (India)

    G. Samuel

  2. Radiopharmaceuticals Operation, BRIT, New Bombay, (India)

    N. Sivaprasad

  3. Chemistry and Industrial Application Section, International Atomic Energy Agency, Wagramerstrasse 5, P.O.Box 200, A-1400, Vienna, (Austria)

    R. S. Mani

Authors
  1. G. Samuel
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  2. N. Sivaprasad
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  3. R. S. Mani
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Samuel, G., Sivaprasad, N. & Mani, R.S. Radioimmunoassay of phenytoin. Journal of Radioanalytical and Nuclear Chemistry, Articles 149, 3–11 (1991). https://doi.org/10.1007/BF02053707

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  • DOI: https://doi.org/10.1007/BF02053707

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