Mycopathologia et mycologia applicata

, Volume 39, Issue 2, pp 113–120 | Cite as

Serological and skin test cross-reactivity between Histoplasma capsulatum and Oidiodendron kalrai

  • R. P. Tewari
  • C. R. Macpherson
  • T. K. Maitra


Guinea pigs were infected with yeast cells ofHistoplasma capsulatum andOidiodendrom kalrai. They were skin tested 6 and 12 weeks post-infection with both homologous and heterologous antigens. There was almost complete skin test cross-reactivity between histoplasmin and oidiodendrin after 12 but not after 6 weeks. There were also strong serologic cross-reactions among 6 different anti-H. capsulatum hyperimmune sera and oidiodendrin. However, cross-reactions among anti-O. kalrai sera and histoplasmin could not be demonstrated.


Yeast Cell Skin Test Starke Heterologous Antigen Hyperimmune Seron 


Meerschweinchen sind mit Hefezellen vonHistoplasma capsulatum undOidiodendron kalrai infiziert worden. Sie waren nach 6 und 12 Wochen mit homologen und heterologen Antigenen Haut-tested. Es war eine fast völlige Hauttest-Kreuzreaktion zwischen Histoplasmin und Oidiodendrin nach 12 aber nicht nach 6 Wochen. Es gab auch eine starke, serologische Kreuzreaktion unter sechs verschiedenen Anti-H. capsulatum Hyperimmunsera und Oidiodendrin. Jedoch konnte man Kreuzreaktionen unter Anti-O. kalrai Sera und Histoplasmin nicht feststellen.


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  1. Asgari, M. &Conant, N. F. (1964) A preliminary note on interaction of skin test sensitivity between histoplasmin and chrysosporin in experimental animals.Mycopath. Mycol. appl. 23:321–327.Google Scholar
  2. Campbell, Charlotte C. (1965) Problems associated with antigenic analysis ofHistoplasma capsulatum and other mycotic agents.Am. Rev. Res. Dis. Supp. 92:113–118.Google Scholar
  3. Campbell, Charlotte C. (1967) Serology in the respiratory mycoses.Sabouraudia 5:240–259.Google Scholar
  4. Campbell, Charlotte C. &Binkley, G. E. (1953) Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis and blastomycosis and the problems of cross-reactions.J. Lab. Clin. Med. 42:896–906.PubMedGoogle Scholar
  5. Carmichael, J. W. (1962) Chrysosporium and some other Aleuriosporic Hyphocetes.Canad. J. Bot. 40:1137–1173.Google Scholar
  6. Drozdov, A. I. (1964) Strainitel 'naya otsenka allergennykh svoistv raznykh preparatov iz vozbuditelei koktsidioidiza, gistoplazmoza i severo amerikanskogo blastomikoza. (Comparative estimation of allergic properties of preparations from the causal agents of coccidioidomycosis, histoplasmosis, and blastomycosis). Tr. Mikol. Labor. Kafed. Mikrobiol. Leningr. Inst. Usoversh. Vrachei, 41:97–101. Abstracted inRev. Med. Vet. Mycol. 5: no. 1484 b, 1965.Google Scholar
  7. Dyson, J. E. &Evans, E. E. (1955) Delayed hypersensitivity in experimental fungus infection. The skin reactivity of antigens from the yeast phase ofHistoplasma capsulatum.J. Lab. Clin. Med. 45:449–454.PubMedGoogle Scholar
  8. Edwards, P. Q. &Palmer, C. E. (1957). Prevalence of sensitivity to coccidioidin, with reference to specific and non-specific reactions to coccidioidin and to histoplasmin.Dis. Chest. 31:35–60.PubMedGoogle Scholar
  9. Edwards, P. Q., Knight, R. A. &Marcus, S. (1961) Skin sensitivity of human beings toHistoplasma capsulatum andBlastomyces dermatitidis, polysaccharide antigens.Am. Rev. Res. Dis. 83:528–534.Google Scholar
  10. Emmons, C. W., Olson, B. J. &Eldridge, W. W. (1945) Studies of the fungi in pulmonary disease. I. Cross-reactions of histoplasmin.Publ. Health Rep. 60:1383–1394.Google Scholar
  11. Glusker, D., Villalobos, F. &Campo, C. (1950) Ocurrencia de intradermorreacciones a la coccidioidina, brulina, histoplasmina, haplosporangina y tuberculina, correlacion a los rayos x, en conscriptos del Ejercito Mexicano. (Occurrence of intradermal reactions to coccidioidin, brucellin, histoplasmin, haplosporangin and tuberculin, in relation to x-rays, among conscripts of the Mexican Army).Bul. Ofic. Sanit. Panamer. 29:715–722. Abstracted inRev. Med. Vet. Mycol. Vol. I, No. 1822, 1952.Google Scholar
  12. Howell, A. (1947) Studies of fungus antigens. I. Quantitative studies of cross-reactions between histoplasmin and blastomycin in guinea pigs.Pub. Health Rep. 62:631–651.Google Scholar
  13. Kaufman, L. &Brandt, B. (1964) Fluorescent antibody studies of the mycelial form ofHistoplasma capsulatum and morphologically similar fungi.J. Bact. 87:120–126.PubMedGoogle Scholar
  14. Knight, R. A., Coray, S. &Marcus, S. (1959)Histoplasma capsulatum andBlastomyces dermatitidis polysaccharide skin tests on humans.Am. Rev. Resp. Dis. 80:264–266.PubMedGoogle Scholar
  15. Labzoffsky, N. A., Fischer, J. B. &Hamoas, J. J. (1957) Studies on the antigenic structure ofHistoplasma capsulatum.Canad. J. Microbiol. 3:975–985.Google Scholar
  16. Palmer, C. E., Edwards, P. Q. &Allfather, W. E. (1957) Characteristics of skin reaction to coccidioidin and histoplasmin with evidence of an unidentified source of sensitization.Am. J. Hyg., 66:196–213.PubMedGoogle Scholar
  17. Salvin, S. B. (1949) The serologic relationship of fungus antigen.J. Lab. Clin. Med. 34:1096–1104.Google Scholar
  18. Salvin, S. B. (1950) Growth of the yeast phase ofHistoplasma capsulatum in a fluid medium.J. Bact. 59:312–313.PubMedGoogle Scholar
  19. Salvin, S. B. (1963) Immunologic aspects of the mycoses.Progr. Allergy 7:213–331.Google Scholar
  20. Salvin, S. B. (1965) Constituents of the cell wall of the yeast phase ofHistoplasma capsulatum.Am. Rev. Resp. Dis. Suppl. 92:119–125.Google Scholar
  21. Saslaw, S. &Campbell, C. C. (1948) A method for demonstrating antibodies in rabbit sera against histoplasmin by collodion agglutination technic.Proc. Soc. Exp. Biol. Med. 68:559–562.Google Scholar
  22. Smith, C. E., Saito, M. T., Beard, R. R., Rosenberger, H. G. &Whiting, E. G. (1949) Histoplasmin sensitivity and coccidioidal infection. I. Occurrence of cross-reactions.Am. J. Pub. Health 39:722–736.Google Scholar
  23. Tompkins, V. N. (1965) Soluble antigenic constituents of yeast phase ofHistoplasma capsulatum.Am. Rev. Resp. Dis. Supp. 92:126–133.Google Scholar
  24. Tewari, R. P. &Macpherson, C. R. (1968) The pathogenicity and neurologic effects ofOidiodendron kalrai for mice.J. Bact. 95:1130–1139.PubMedGoogle Scholar

Copyright information

© Dr. W. Junk N.V. 1969

Authors and Affiliations

  • R. P. Tewari
    • 1
  • C. R. Macpherson
    • 1
  • T. K. Maitra
    • 1
  1. 1.Departments of Microbiology and PathologyOhio State University, College of MedicineColumbus

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