Summary
A single spore culture, obtained from a local Baker's yeast strain belonging toSaccharomyces carlsbergensis, proved to be a double mutant for an aminoacid and a pyrimidine. It required lysine as well as uracil or cytosine for growth. It failed to utilise thymine and 5-methyl cytosine. It thus possesses the capacity of amination and deamination of pyrimidines and of coupling uracil and cytosine with ribose, but lacks the ability to demethylate pyrimidines.
Two other uracil less mutants ofSaccharomyces carlsbergensis, one induced by X-rays and the other by U.V. behaved in the same way as the spontaneous double mutant in respect of the utilisation of uracil, cytosine, thymine and 5-methyl cytosine.
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Ahmad, M., Ahmed, Z.U. Studies on an interesting Saccharomyces carlsbergensis mutant. Mycopathologia et Mycologia Applicata 35, 27–32 (1968). https://doi.org/10.1007/BF02053275
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DOI: https://doi.org/10.1007/BF02053275