Mycopathologia et mycologia applicata

, Volume 46, Issue 4, pp 335–339 | Cite as

Starch utilization by Phytophthora spp

  • H. H. Ho
  • Barbara Foster
Article

Summary

Phytophthora spp. were grown on artificial starch agar medium. In some cases, the capacity of starch utilization could be a useful tool in species separation ofPhytophthora. Based on the ability to hydrolyse starch,P. palmivora andP. parasitica could be readily distinguished, whereasP. parasitica andP. parasitica var.nicotianae, P. megasperma andP. megasperma var.sojae (P. sojae) behaved similarly. Starch hydrolysis was indicated by a clear unstained zone within the fungal colony when treated with iodine solution. Simple quantitative analysis of starch hydrolysis was made feasible by the following formula:

Starch Hydrolysis Index (S.H.I.) = Mean diameter of clear starch hydrolysis zone (d) / Mean diameter of fungal colony (D)

Keywords

Hydrolysis Starch Agar Iodine Agar Medium 

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References

  1. Christie, T. (1958) Nutritional studies ofPhytophthora cactorum (Leb &Cohn)Shroet.New Zealand J. Science 1:83–90.Google Scholar
  2. Davis, M. E. (1959) The nutrition ofPhytophthora fragariae.Brit. Mycol. Soc. Trans. 42:193–200.Google Scholar
  3. Erwin, D. C., Zentmyer, G. A., Galindo, J. &Niederhauser, J. S. (1963) Variation in the genusPhytophthora.Ann. Rev. Phytopath. 1:375–396.Google Scholar
  4. Fergus, C. L. (1969) The production of amylase by some thermophilic fungi.Mycologia 61:1171–1175.Google Scholar
  5. Gallegly, M. E. (1970) Genetics ofPhytophthora.Phytopathology 60:1135–1141.Google Scholar
  6. Hall, R., Zentmyer, G. A. &Erwin, D. C. (1969) Approach to taxonomy ofPhytophthora through acrylamide gel-electrophoresis of proteins.Phytopathology 59:770–774.Google Scholar
  7. Hickman, C. J. (1958)Phytophthora — plant destroyer.Brit. Mycol. Soc. Trans. 41,1–13.Google Scholar
  8. Hickman, C. J. (1970) Biology ofPhytophthora zoospores.Phytopathology 60:1128–1135.Google Scholar
  9. Ho, H. H. &Hickman, C. J. (1967) Asexual reproduction and behavior of zoospores ofPhytophthora megasperma var.sojae.Can. J. Botany 45:1963–1981.Google Scholar
  10. Liang, Ping-Yen &Lin, Ch'wan-Kwang (1965) Starch utilization capability as a diagnostic character for species separation inPhytophthora.Acta Phytopath. Sinica 8(1):57–60. (In Chinese, with English summary).Google Scholar
  11. Mehrotra, B. S. (1951) Physiological studies of some Phytophthoras III. Carbon requirements.Lloydia 14:122–128.Google Scholar
  12. Tucker, C. M. (1931) Taxonomy of the genusPhytophthora de Bary.Missouri Univ. Agr. Exp. Sta. Res. Bull. No. 153, 208 p.Google Scholar
  13. Waterhouse, G. M. (1963) Key to the species ofPhytophthora de Bary.Commonwealth Mycol. Inst. Mycol. Papers No. 92, 22 p.Google Scholar
  14. Waterhouse, G. M. (1970) Taxonomy inPhytophthora.Phytopathology 60:1141–1143.Google Scholar
  15. Wills, W. H. (1954) The utilization of carbon and nitrogen compounds byPhytophthora parasitica Dastur var.nicotianae (Breda de Haan)Tucker.J. Elisha Mitchell Sci. Soc. 70:231–235.Google Scholar
  16. Zentmyer, G. A. &Erwin, D. C. (1970) Development and reproduction ofPhytophthora.Phytopathology 60:1120–1127.Google Scholar

Copyright information

© Dr. W. Junk N. V. 1972

Authors and Affiliations

  • H. H. Ho
    • 1
  • Barbara Foster
    • 1
  1. 1.Department of BiologyState University CollegeNew Paltz

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