Abstract
We have noticed important metabolic modifications during the first 15 hours of the sporulation process of yeast in contact with acetic acid. Carbohydrate reserves, dry weight and proteins increase. On the other hand, the activity of fumarase and isocitritase in cell homogenates are hardly altered. The results prove true the hypothesis according to which the metabolism of acetic acid is altered during sporulation.
Zusammenfassung
Beträchtliche metabolische Veränderungen wurden bei Hefe, während der fünfzehn ersten Stunden der Sporenbildung unter Zugabe von Essigsäure, beobachtet. Der Kohlenhydratenvorrat, die Trockensubstanz und der Proteingehalt nehmen zu. Die Aktivität der Fumarase und Isocitritase wird kaum verändert. Dadurch wird die Annahme bestätigt, daß eine Abweichung des Metabolismus der Essigsäure während der Sporenbildung stattfindet.
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Références
Croes A. F. (1967). Induction of meiosis in yeast. I. Timing of cytological and biochemical events.Planta (Berl) 76:209–226.
Dixon G. H. &Kornberg H. L. (1959). Assay method for key enzymes of the glyoxylate cycle.Biochem. J. 72:3P.
Duntze W., Neumann D., Gancedo J. M., Atzpodien W. &Holzer H. (1969). Studies on the regulation and localization of the glyoxylate enzymes inSaccharomyces cerevisiae.European J. Biochem. 10:83–89.
Esposito M. S., Esposito R. E., Arnaud M. &Halvorson H. O. (1969). Acetate utilization and macromolecular synthesis during sporulation of yeast.J. Bacteriol. 100:180–186.
Galzy P. &Bizeau C. (1966). Action de l'acide acétique sur la sporulation deSaccharomyces cerevisiae Hansen.C. R. Soc. Biol. 160:176–178.
Hirsch H. M. (1952). A comparative study of aconitase, fumarase and DPN-linked isocitric dehydrogenase in normal and respiration deficient yeast.Biochim. Bicphys. Acta 9:674–686.
Racker E. (1950).Biochim. Biophys. Acta 4:2II.
Roth R. (1970). Carbohydrate accumulation during the sporulation of yeast.J. Bacteriol. 101:53–57.
Roth R. &Halvorson H. O. (1969). Sporulation of yeast harvested during logarithmic growth.J. Bacteriol. 98:831–832.
Somlo M. (1968). Induction and repression of mitochondrial ATP ase in yeast.European J. Biochem. 5:276–284.
Strickland L. H. (1951). The determination of small quantities of bacteria by means of the biuret reaction.J. Gen. Microbiol. 5:698.
Vary M. J., Edwards C. L. &Stewart P. R. (1969). The biogenesis of mitochondria. IX. Formation of the soluble mitochondrial enzymes malate deshydrogenase and fumarase inSaccharomyces cerevisiae.Arch. Biochem. Biophys. 130:235–243.
Vezinhet Françoise (1970). Contribution à l'étude de la sporulation chezSaccharomyces cerevisiae Hansen.Mycopath. Mycol. Appl. 40:15–48.
Vezinhet F., Arnaud A. &Galzy P. (1971). Etude de la respiration de la levure au cours de la sporulation (à paraître).
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Arnaud, A., Vezinhet, F. & Galzy, P. Remarques sur le métabolisme de l'acide acétique chez la levure au cours de la sporulation. Mycopathologia et Mycologia Applicata 45, 231–242 (1971). https://doi.org/10.1007/BF02051970
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DOI: https://doi.org/10.1007/BF02051970