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A serological comparison of the three morphological phases of coccidioides immitis: Complement fixation tests with a Pooled antiserum obtained from rabbits with experimental Coccidioidomycosis

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Summary

A sonicated spherule preparation was more reactive than a sonicated arthrospore antigen in complement fixation tests with a pooled serum from rabbits with experimental coccidioidomycosis. The reactivity of the sonicated spherule approximated the reactivity of coccidioidin. When the sonicated spherule was separated into its supernatant and sediment fractions, both preparations exhibited serological activity.

Zusammenfassung

Ein mit Schallwellen hergestelltes Kügelchen-Präparat war activer in dem Komplement-Tests mit Blutserum von Kaninchen mit einer Coccidioidomycosisinfektion als ein mit Schallwellen hergestelltes Arthrosporantigen. Die Reaktivität der mit Schallwellen hergestellten Kügelchen war der des Coccidioidin ähnlich. Wurden diese Kügelchen in Niederschlag und Lösung getrennt, so hatten beide Präparate serologische Aktivität.

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Author notes

  1. M. E. Landay

    Present address: Dept. of Epidemiology and Environmental Health, The George Washington Univ. Medical School, 20005, Washington D.C.

Authors and Affiliations

  1. Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina

    M. E. Landay, R. W. Wheat, N. F. Conant & E. P. Lowe

  2. Commodity Development and Engineering Laboratory, Fort Detrick, Frederick, Maryland

    M. E. Landay, R. W. Wheat, N. F. Conant & E. P. Lowe

Authors
  1. M. E. Landay
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  2. R. W. Wheat
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  3. N. F. Conant
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  4. E. P. Lowe
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Additional information

Part of a dissertation submitted to the Graduate School of Duke University in partial fulfillment of requirements for the Ph.D. degree.

This work was supported by contract with the Department of the Army, Fort Detrick, Frederick, Maryland.

In conducting the research reported herein, the investigators adhered to “Guide for Laboratory Animal Facilities and Care” established by the Committee on the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources, NAS—NRC.

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Landay, M.E., Wheat, R.W., Conant, N.F. et al. A serological comparison of the three morphological phases of coccidioides immitis: Complement fixation tests with a Pooled antiserum obtained from rabbits with experimental Coccidioidomycosis. Mycopathologia et Mycologia Applicata 34, 289–295 (1968). https://doi.org/10.1007/BF02051853

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  • DOI: https://doi.org/10.1007/BF02051853

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