Summary
1) Extract was obtained by repeated cryolysis of acetone-dried mycelia ofAsp. fumigatus killed by formalin. High titer immune serum could be easily obtained by immunizing rabbits with this extract.
2) FI antigen prepared from mycelial extract by alcoholic precipitation exhibited species-specificity when examined by the precipitin test.
3) FII, prepared from FI by chloroform-butanol treatment, was no better than FI in antigenic activity.
4) FI could be used as antigen in the skin test with rabbits infected withAsp. fumigatus, when the final reading was based on the redness (larger than 5 mm in diameter) 36 hours after the injection of antigen.
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Fukui, M., Yasuda, J. Serological studies on Aspergillus fumigatus. Mycopathologia et Mycologia Applicata 14, 39–54 (1961). https://doi.org/10.1007/BF02051436
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DOI: https://doi.org/10.1007/BF02051436