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Specific fluorescent antiglobulins for the detection and identification of blastomyces dermatitidis yeast-phase cells

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Summary

Two lots of rabbit anti-Blastomyces dermatitidis globulins were conjugated with fluorescein isothiocyanate. These reagents stained brightly elements of the yeast and mycelial phases of 10 strains ofB. dermatitidis. In addition, the labeled antibodies cross-reacted with elements of the yeast and mycelial phases of 7 strains ofHistoplasma capsulatum and cells of numerous other heterologous fungi. Adsorption of one lot of labeled antibodies twice with yeast cells ofH. capsulatum and once with elements ofGeotrichum candidum rendered the conjugate specific for the yeast phase ofB. dermatitidis. Three adsorptions with yeast cells ofH. capsulatum followed by a single adsorption with elements ofG. candidum rendered the second conjugate specific for yeast-phase cells ofB. dermatitidis. The specific reagents did not react with the mycelial phase of this fungus.

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References

  1. Binford, C.H. (1955): Histoplasmosis; tissue reactions and morphologic variations of the fungus, Amer. J. clin. Path.,25: 25–36.

    Google Scholar 

  2. Campbell, C. C. &Binkley, G. E. (1953): Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis and blastomycosis and the problem of cross reactions, J. Lab. clin. Med.42: 896–906.

    Google Scholar 

  3. Campbell, C. C. (1960): The accuracy of serologic methods in diagnosis, Ann. New York Acad. Sci.,89: 163–177.

    Google Scholar 

  4. Goldman, M. (1957): StainingToxoplasma gondii with fluorescein-labeled antibody. II. A new serologic test for antibodies toToxoplasm based upon inhibition of specific staining, J. exp. Med.,105: 557–573.

    Google Scholar 

  5. Goldwasser, R. A. &Shephard, C. C. (1958): Staining of complement and modifications of fluorescent antibody procedures, J. Immunol.,80: 122–131.

    Google Scholar 

  6. Gordon, M. A. (1958): Differentiation of yeasts by means of fluorescent antibody, Proc. Soc. exp. Biol. Med.,97: 694–698.

    Google Scholar 

  7. Gordon, M. A. (1958): Rapid serological differentiation ofCandida albicans fromCandida stellatoidea, J. invest. Derm.,31: 123–125.

    Google Scholar 

  8. Gordon, M. A. (1959): Fluorescent staining ofHistoplasma capsulatum, J. Bact.,77: 678–681.

    Google Scholar 

  9. Kaplan, W. &Ivens, M. S. (1960): Fluorescent antibody staining ofSporotrichum schenckii in cultures and clinical materials, J. invest. Derm.,35: 151–159.

    Google Scholar 

  10. Kaufman, L. &Kaplan, W. (1961): Preparation of a fluorescent antibody specific for the yeast phase ofHistoplasma capsulatum, J. Bact.,82: 729–735.

    Google Scholar 

  11. Kaufman, L. &Cherry, W. B. (1961) Technical factors affecting the preparation of fluorescent antibody reagents, J. Immunol.,87: 72–79.

    Google Scholar 

  12. Moody, M. D., Goldman, M. &Thomason, B. M. (1956): Staining bacterial smears with fluorescent antibody. I. General methods forMalleomyces pseudomallei, J. Bact.,72: 357–361.

    Google Scholar 

  13. Moore, M. (1955): Morphologic variation in tissue of the organisms of the blastomycoses and of histoplasmosis, Amer. J. Path.,31: 1049–1064.

    Google Scholar 

  14. Riggs, J. L., Seiwald, R. J., Burckhalter, J., Downs, C. M. &Metcalf, T. G. (1958): Isothiocyanate compounds as fluorescent labeling agents for immune serum, Amer. J. Path.,34: 1081–1097.

    Google Scholar 

  15. Salvin, S. B. (1949): The serologic relationship of fungus antigens, J. Lab. clin. Med.,34: 1096–1104.

    Google Scholar 

  16. Seeliger, H. P. R. (1962): Serology of fungi and deep fungous infections, Fungi and Fungous Diseases, Springfield, Ill., Charles C. Thomas, 158–186.

  17. Starr, G. F., Dawe, C. J. &Weed, L. A. (1955): Use of periodic acid-Schiff stain in identification of pathogenic fungi in tissues, Amer. J. clin. Path.,25: 76–83.

    Google Scholar 

  18. Weed, L. A. (1955): North American blastomycosis, Amer. J. clin. Path.,25: 37–44.

    Google Scholar 

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Authors and Affiliations

  1. From the Communicable Disease Center, Public Health Service, U. S. Department of Health, Education and Welfare, Atlanta 22, Georgia

    William Kaplan D.V.M. & Leo Kaufman Ph. D.

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  1. William Kaplan D.V.M.
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  2. Leo Kaufman Ph. D.
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Kaplan, W., Kaufman, L. Specific fluorescent antiglobulins for the detection and identification of blastomyces dermatitidis yeast-phase cells. Mycopathologia et Mycologia Applicata 19, 173–180 (1963). https://doi.org/10.1007/BF02051245

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