Summary
32 sputa from 29 patients known to have yeasts were emulsified and quantitatively plated on Sabouraud's glucose agar. Colony counts showed that the yeast flora varied from about 300 to about one million cells per ml of the specimen. Sputa collected at bed-side and plated within 2 hours gave lower counts than those received routinely. In the 28 specimens in which identification was carried outC. albicans was found in 23 as the only or the dominant species andC. tropicalis in 4; an unidentified species was present in one specimen.
Similar content being viewed by others
References
Blank, H. 1957.Candida albicans, frequent companion, occasional foe. Monogr. on Ther.2: 1–6.
Brown, T. G. 1946. Pulmonary mycosis. Edinb. med. J.53: 583–8.
Burns, J. &Larsh, H. W. 1962. Selective isolation ofHistoplasma capsulatum in presence ofCandida spp. A.S.M. Bact. Proc. p. 66.
Csillag, A., Vince, I. &Simon, G. 1958. Occurrence ofCandida albicans in the throat-mucus of healthy and diseased infants. Ann. paediat. (Basel)190: 352–61.
Halde, C., Wright, E. T., Pollard, W. H., Newcomer, V. D. &Sternberg, T. H. 1957. The effect of amphotheracin B upon the yeast flora of the gastrointestinal tract of man. Antibiot. Ann. 1956–57, 123–27.
Hewitt, W. L., Finegold, S. M. &Sutter, V. L. 1956. Incidence of side effects and changes in fecal microflora following administration of tetracycline and tetracycline-nystatin. Anitibiot. Ann., 1955–56, 856–61.
Kapica, L., Maciejewska, J. &Nielsen, G. E. 1964. An unusual strain ofHistoplasma capsulatum isolated in Montreal. Paper read at the 32nd Ann. Christmas Meeting, Lab. Sec., Canad. Publ. Hlth. Assoc. Montebello, P.Q. Canada.
Keiper, T. W. 1938. Studies on yeastlike fungi isolated from pulmonary disease (bronchomoniliasis) J. Lab. clin. Med.23: 343–54.
Kozinn, P. J. &Taschdjian, C. L. 1962. Enteric candidiasis: diagnosis and clinical considerations. Pediatrics30: 71–85.
Lodder, J. & Kreger-Van Rij,N. G. W. 1952. The Yeasts. N-Holland Publ. Co. Amsterdam.
Metzger, W. I., Steigman, F., Jenkins, C. J. Jr., Pamukcu, S. F. &Kaminski, L. 1957. Comparative effects of tetracycline alone and in combination with nystatin on the intestinal flora of man. Antibiot. Ann., 1956–57, 208–15.
Newcomer, V. D., Wright, E. T., &Sternberg, T. H. 1955. The effect of nystatin when administered simultaneously with tetracycline upon the yeast flora of the gastrointestinal tract of man. Antibiot. Ann., 1954–55, 686–90.
Orie, N. G. M. 1952.Candida (Monilia) infection of the respiratory tract. Dis. Chest.22: 107–17.
Paine, T. F. 1958. The inhibitory action of bacteria onCandida growth. Antibiot. and Chemother.8: 273–81.
Reiersol, S. 1958. Occurrence of yeasts in the intestinal tract of 20 patients during treatment with bacitracin and neomycin. Antonie v. Leeuwenhoek24: 23–26.
Schwarting, V. M. &Skinner, C. E. 1949.Candida in sputum of patients with tuberculosis. Mycopathologia4: 349–55.
Staib, F. &Braun, H. 1961.Candida tropicalis bei pathologischen Lungenbefunden. Dtsch. med. Wschr.86: 940–44, 970–71.
Sternberg, T. H., Tarbet, J. E., Newcomer, V. D., Huddleson, H. G., Wright, E. T., &Egeberg, R. C. 1954. Antifungal effects on nystatin on fecal flora in animals and men. Antibiot. Ann., 1953–54, 199–209.
Author information
Authors and Affiliations
Additional information
This work was supported by a Grant from the Medical Research Council, Ottawa, Canada.
Rights and permissions
About this article
Cite this article
Kapica, L., Clifford, A. Quantitative determination of yeasts in sputum. Mycopathologia et Mycologia Applicata 34, 27–32 (1968). https://doi.org/10.1007/BF02050841
Issue Date:
DOI: https://doi.org/10.1007/BF02050841