Summary
The effect of temperature on formation of aflatoxin on solid substrate (rice) byAspergillus flavus NRRL 2999 has been studied in some detail. The optimum temperature for production of both aflatoxin B1 and G1 under the conditions employed is 28° C. Comparable yields of B1 were obtained at 32° C, but considerably less G1 was produced at this temperature. Both B1 and G1 were found in lesser amounts at temperatures above 32° C, and the aflatoxin content of rice incubated at 37° C was low (300–700 ppb) even though growth was good.
Reducing the temperature from 28° to 15° C resulted in progressively less aflatoxin, but 100 ppb of B1 was detected in cultures incubated 3 weeks at 11° C. No aflatoxin was produced at 8° C.
The ratio of the four aflatoxins is affected by temperature. At the lower temperatures, essentially equal amounts of aflatoxin B1 and G1 were produced, whereas at 28° C, approximately four times as much B1 was detected as G1. At the higher temperatures, relatively less G was formed, until at 37° C, less than 10 ppb was detected.
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This is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture.
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Sorenson, W.G., Hesseltine, C.W. & Shotwell, O.L. Effect of temperature on production of aflatoxin on rice by Aspergillus flavus. Mycopathologia et Mycologia Applicata 33, 49–55 (1967). https://doi.org/10.1007/BF02049790
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DOI: https://doi.org/10.1007/BF02049790