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Mycopathologia et mycologia applicata

, Volume 36, Issue 2, pp 150–160 | Cite as

Spontaneous mutants of Microsporon gypseum resistant to griseofulvin

  • Karel Lenhart
Article

Summary

1) The spores of the microconidial mutant I–18 of the dermatophyteMicrosporon gypseum in agar medium with GF germinated and formed germ tubes deformated in a characteristic way. From 1µg GF/ml up with an increasing antibiotic concentration (expressed in logarithms) the munber of colonies grown (expressed in probits) decreased linearly.

2) As a sensitivity measure of the spores the median efficient dose ED 50 was used which was determined by means of a graphic probit analysis. For the strain used this value was determined in the range between 1.35–1.95µg GF/ml in three independent experiments.

3) From the smears of a thickened spore suspension (1.6–14.2 × 107 viable spores) in medium containing a high GF concentration a very small, but as for the order a stable number of colonies grew, as found in eight independent experiments. On the medium containing 20µg GF/ml in average 61 colonies grew, on 40µg GF/ml 20 colonies, on 80µg GF/ml 3 colonies and on 160µg GF/ml 0.3 colony (expressed in 107 viable spores tested).

4) A part of these colonies were isolated and transferred 29 times on a medium without the antibiotic. Two isolates only show a permanently increased resistance to GF, viz. the strain D-29 which is 50 × more resistant and the strain N-53 which is 3.5 × more resistant than the wild strain I-18.

Keywords

Germinate Independent Experiment Agar Medium Sensitivity Measure Spore Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ashida, J. 1965. Adaptation of fungi to metal toxicants. Ann. Rev. Phytopath.3: 153–174.Google Scholar
  2. Barron, G. L. 1962. The parasexual cycle and linkage relationships in the storage rot fungus Penicillium expansum. Canad. J. Bot.40: 1603–1613.Google Scholar
  3. Bryson, V., &Szybalski, W. 1955. Microbial drug resistance. Adv. Genetics7: 1–46.Google Scholar
  4. Calvori, C. &Morpurgo, G. 1966. On the nature of UV and HNO2 induced mutations in Aspergillus nidulans. Abstract in: “Proceedings of a symposium on the mutational process” (Praha, August 9–11, 1965), p. 341–342.Google Scholar
  5. Carter, G. A. &Wain, R. L. Investigations on fungicides. IX. The fungitoxicity, phytotoxicity, and systemic fungicidal activity of some inorganic salts. Ann. appl. Biol.53: 291–309.Google Scholar
  6. Dutta, S. K. &Garber, E. D. 1962. Genetics of phytopathogenic fungi. VII. Fungicide resistance in Colletotrichum lagenarium. Phytopathology52: 35–38.Google Scholar
  7. Eagle, H., Fleischman, R. &Levy, M. 1952. Development of increased bacterial resistance to antibiotics. I. Continuous spectrum of resistance to penicillin, chloramphenicol, and streptomycin. J. Bact.63: 623–638.PubMedGoogle Scholar
  8. Esser, K. &Kuenen, R. 1965. Genetik der Pilze. Springer Verlag.Google Scholar
  9. Fincham, J. R. S. &Day, P. R. 1963. Fungal genetics. Oxford.Google Scholar
  10. Frick, E. L. 1964. Methods of reducing variability in the results of glass-slide spore germination assays of fungitoxicity. Ann. appl. Biol.54: 349–360.Google Scholar
  11. Gale, E. F. &Davies, R. (Eds.) 1953. Adaptation in microorganisms. London.Google Scholar
  12. Georgopoulos, S. G. 1962. Genetic nature of tolerance of Hypomyces solani f. cucurbitae to penta — and tetra-chlorinated nitrobenzenes. Nature (London)194: 148–149.Google Scholar
  13. Georgopoulos, S. G. 1963. Tolerance to chlorinated nitrobenzenes in Hypomyces solani f. cucurbitae and its mode of inheritance. Phytopathology53: 1086–1093.Google Scholar
  14. Hejtmánek, M., Hejtmánková-Uhrová, N., Kunert, J. Lenhart, K. &Vičiková, L. 1967. Strahleninduzierte Mutationen bei Dermatophyten. I. Morphogenetische Mutanten. Mycopathol. et Mycol. appl.33: 321–337.Google Scholar
  15. Horsfall, J. G. Fungicides and their action. Waltham 1945.Google Scholar
  16. McIntosh, A. H. 1961. Some variants and possible errors in the test — tube dilution and slide — germination methods for laboratory testing of fungicides. Ann. appl. Biol.49: 424–432.Google Scholar
  17. Kirby, A. H. M. &Frick, E. L. 1952. Modifications in the glass-slide spore-germination test for the use in fungicide research. Rep. E. Malling Res. Sta. 161–167.Google Scholar
  18. Lachowicz, T. M. 1960. Mechanizm powstawania form opornych na penicyline. Acta Microbiol. Polon.9: 107–132.Google Scholar
  19. Lenhart, K. 1968. Determination of griseofulvin sensitivity by mycelial growth test. Mykosen11: 195–204.PubMedGoogle Scholar
  20. Lenhart, K. Griseofulvin as a mutagenic factor (In press).Google Scholar
  21. Ogawa, J. M., Ramsey, R. H. &Moore, C. J. 1963. Behavior of variants of Gilbertella persicaria arising in medium containing 2,6 — dichloro — 4 — nitroaniline. Phytopathology53: 97–100.Google Scholar
  22. Priest, D. &Wood, R. K. S. 1961. Strains of Botrytis allii resistant to chlorinated nitrobenzenes. Ann. appl. Biol.49: 445–460.Google Scholar
  23. Prušová, H. 1962. Methode der getrockenen Tropfen der Fungizidensuspension. (Ein Beitrag zur Vereinfachung der Prüfung von Fungizidenwirksamkeit). Čes. Mykol.16: 214–218.Google Scholar
  24. Roper, J. A. &Kafer, E. 1957. Acriflavine resistant mutants of Aspergillus nidulans. J. gen. Microbiol.16: 660–667.PubMedGoogle Scholar
  25. Roth, Z., Josifko, M., Maly, V. &Trčka, V. 1962. Statistické metody ex perimentální medicíně. Praha.Google Scholar
  26. Sevag, M. G., Reid, R. D. &Reynolds, D. E. (Eds.) 1955. Origins of resistance to toxic agents. Acad. Press.Google Scholar
  27. Somers, E. 1961. The fungitoxicity of metal ions. Ann. appl. Biol.49: 246–253.Google Scholar
  28. Tinline, R. D. 1961. Cochliobolus sativus. IV. Drug — resistant, color, and nutritionally exacting mutants. Canad. J. Bot.39: 1695–1704.Google Scholar
  29. Wolstenholme, G. E. W. &O'Connor, C. M. (Eds.) 1957. Drug resistance in microorganisms. London.Google Scholar

Copyright information

© Dr. W. Junk N.V. 1968

Authors and Affiliations

  • Karel Lenhart
    • 1
  1. 1.Department of BiologyMedical FacultyOlomoucCzechoslovakia

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