Mycopathologia et mycologia applicata

, Volume 36, Issue 2, pp 150–160 | Cite as

Spontaneous mutants of Microsporon gypseum resistant to griseofulvin

  • Karel Lenhart


1) The spores of the microconidial mutant I–18 of the dermatophyteMicrosporon gypseum in agar medium with GF germinated and formed germ tubes deformated in a characteristic way. From 1µg GF/ml up with an increasing antibiotic concentration (expressed in logarithms) the munber of colonies grown (expressed in probits) decreased linearly.

2) As a sensitivity measure of the spores the median efficient dose ED 50 was used which was determined by means of a graphic probit analysis. For the strain used this value was determined in the range between 1.35–1.95µg GF/ml in three independent experiments.

3) From the smears of a thickened spore suspension (1.6–14.2 × 107 viable spores) in medium containing a high GF concentration a very small, but as for the order a stable number of colonies grew, as found in eight independent experiments. On the medium containing 20µg GF/ml in average 61 colonies grew, on 40µg GF/ml 20 colonies, on 80µg GF/ml 3 colonies and on 160µg GF/ml 0.3 colony (expressed in 107 viable spores tested).

4) A part of these colonies were isolated and transferred 29 times on a medium without the antibiotic. Two isolates only show a permanently increased resistance to GF, viz. the strain D-29 which is 50 × more resistant and the strain N-53 which is 3.5 × more resistant than the wild strain I-18.


Germinate Independent Experiment Agar Medium Sensitivity Measure Spore Suspension 
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Copyright information

© Dr. W. Junk N.V. 1968

Authors and Affiliations

  • Karel Lenhart
    • 1
  1. 1.Department of BiologyMedical FacultyOlomoucCzechoslovakia

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