Summary
Amylase activity in the acetone-dried preparations of floating mycelial pads ofT. rubrum andT. mentagrophytes was investigated. The kinetics of the enzyme and the effects of metal ions and metal binders are also reported. The amylase was more active inT. rubrum than inT. mentagrophytes a fact which is correlated with their pathogenicity and distribution. The optimum pH was found to be 4.8 and the enzyme was almost completely inactivated at 80°C. Mn++, Cu++, and Hg++ in concentrations of 0.001 M inhibited the enzyme action. Co++, 8-hydroxyquinoline, hydrazine sulphate, α, α′-dipyridyl, hydroxylamine, sodium azide, o-phenanthroline and EDTA accelerated it in the order mentioned.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Beilstein (1951) Beilstein's Handbuch der Organischen Chemie. Isocyclische Reihe. Verlag von Julius Springer, Berlin. Band X. 1066: 122.
Bose, S. R. &Sarkar, S. N. (1937) Enzymes of some woodrotting polypores. Proc. Roy. Soc. London, Series B.123: 193–213.
Chattaway, F. W., Thomson, C. C. &Barlow, A. J. E. (1954) Enzymes ofMicrosporon canis. Biochim. Biophys. Acta,14: 583.
Das-Gupta, S. N. &Shome, S. K. (1959) Studies in medical mycology. I. On the occurrence of mycotic diseases in Lucknow. Mycopathol. et Mycol. Appl.10: 177–186.
Das-Gupta, S. N. &Shome, S. K. (1960) Studies in medical mycology. VI. A note on the enzymes of two common dermatophytes. Curr. Sci.29: 432–433.
Fults, J. L., Schaal, L. A. &Michaelson, M. R. (1948) Value of 2,3,5-triphenyl tetrazolium chloride reaction and ultraviolet light in parasitism studies of strains ofActinomyces scabies. Soil Sci. Soc. Amer. Proc.13: 287–291.
Goddard, D. R. (1934) Phases of metabolism ofTrichophyton interdigitale. Priestley J. infect. Dis.54: 149–163.
Gunsalus, I. C. (1955) Extraction of enzymes from microorganisms (Bacteria and yeast), in Colowick, S. P. and Kaplan, N. O. Ed. ‘Methods in Enzymology’ Academic Press, New York, 1955. Vol. I: 51–62.
Leopold, H. &Starbanow, M. P. (1943) The nature of amylase fromRhizopus japonicus (Amylomyces-β). Biochem. Z.314: 232–249.
MacFadyen, A. (1896) A contribution to the biology of ringworm organism. J. Path. Bact.3: 177–183.
Noelting, G. &Bernfeld, P. (1948) Sur les enzymes amylolytiques (III). La amylase: dosage d'activité et contrôle de l'absence d'amylase. Helv. chim. Acta,31: 286–290.
Okazaki, H. (1954) Amylase of various species of molds. VIII. Production and properties of saccharogenic amylase ofAspergillus oryzae. Nippon Nogei Kagaku Kaishi,28: 51–56.
Okazaki, H. (1953) Properties of saccharogenic amylase ofAspergillus oryzae. J. Agr. Chem. Soc. Japan,27: 296.
Okazaki, H. (1955) Saccharogenic amylase from cultures ofAspergillus oryzae. Japan, 3038 ('55), May 4.
Roberts, L. (1899) Experimental notes on the ferments of ringworm fungi. Brit. med. J. Jan.7: 13–14.
Sakamoto, M. &Shuzui, K. (1957) The enzymes produced by microorganisms and their utilisation. III. The influence of various substances on the protease and amylase of aspergilli. Hakko-Kogaku Zasshi,35: 182–187.
Sen, C. (1964) Enzyme make-up ofTrichophyton rubrum andT. mentagrophytes. I. Aldolase activity. Mycopathol. et Mycol. Appl.24, 3: 211–219.
Tate, P. (1929) On the enzymes of certain dermatophytes or ringworm fungi. Parasitology,21: 31–54.
Vyatkin, V. V. (1959) Effect of magnesium salts on the enzymic activity of deep cultures ofAspergillus niger. Trudy Inst. Mikrobiol., Akad. Nauk S.S.S.R. No.6: 150–156.
Yamamoto, I., Kawata, K. &Watanabe, S. (1957) Acta Scol. Med. Gifu,5: 529–533.
Ito, Y. &Fujii, T. (1958) Hydrolytic enzymes of dermatophytes. Rend. ist. lombardo Sci. Pt. I. Classe Sci. mat. e nat.92B: 301–312.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sen, C. Enzyme make-up of trichophyton rubrum and t. mentagrophytes. Mycopathologia et Mycologia Applicata 24, 220–227 (1964). https://doi.org/10.1007/BF02049282
Issue Date:
DOI: https://doi.org/10.1007/BF02049282