Acta Diabetologica

, Volume 33, Issue 3, pp 236–240 | Cite as

A diet enriched in protein accelerates diabetes manifestation in NOD mice

  • K. Schneider
  • H. Laube
  • T. Linn
Original

Abstract

Diet modifies the development of insulin-dependent diabetes mellitus in animals and in humans. We examined female non-obese-diabetic (NOD) mice, a diabetes-prone mouse strain with 70% spontaneous diabetes incidence and metabolic abnormalities in non-overtly diabetic litters. They were fed a diet containing 55% (n=27) or 15% (n=26) protein, respectively, after weaning. At an age of 30 weeks, non-diabetic NOD mice were submitted to an intravenous glucose tolerance test (0.5 g/kg body weight; blood samples were taken after 2, 4, 8, 10, 15, 20 and 30 min) and to perfusion of the pancreas (stimulation media were Krebs-Ringer-Hepes buffer with 5 mmol/l glucose, 30 mmol/l glucose and 5 mmol/l glucose plus 19 mmol/l arginine). Diabetic mice were removed from the experiment. Serum glucose concentration and body weight were monitored weekly. Food ingestion was checked at an age of 11 weeks. On average, the onset of diabetes was diagnosed in mice on a high-protein diet (19.7±1.3 weeks) 4 weeks earlier than in mice on a low-protein diet (23.5±1.1 weeks;P<0.05). Non-diabetic NOD mice on a high-protein diet showed significantly better glucose tolerance (as determined by the glucose disappearance rate) and mean insulin secretion (at 30 mmol/l glucose). No difference in the serum glucose concentration between non-diabetic mice on the low-protein diet or high-protein diet could be proved. In non-diabetic mice on the high-protein diet the body weight and food ingestion exceeded those of mice on the low-protein diet (P<0.05). High insulin secretion and glucose tolerance in non-diabetic mice may reflect the capacity of beta-cells to adapt; however, beta-cells tend to be destroyed under such circumstances. Thus, a high-protein diet promoted the onset of diabetes, but it did not increase significantly the incidence of the disease.

Key words

Non-obese-diabetic (NOD) mouse High protein diet Insulin secretion Perfusion 

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References

  1. Atkinson MA, Winter WE, Skordis M, Beppu H, Riley WM, Maclaren NK, Dietary protein restriction reduces the frequency and delays the onset of insulin dependent diabetes in BB rats. Autoimmunity 2:11–20, 1988PubMedGoogle Scholar
  2. Baxter AG, Koulmanda M, Mandel TE, High and low diabetes incidence nonobese diabetic (NOD) mice: origins and characterisation. Autoimmunity 9:61–67, 1991PubMedGoogle Scholar
  3. Coleman DL, Kuzava JE, Leiter EH, Effect of diet on incidence of diabetes in nonobese diabetic mice. Diabetes 39:432–436, 1990PubMedGoogle Scholar
  4. Eisenbarth GS, Type I diabetes mellitus: a chronic autoimmune disease. N Engl J Med 314:1360–1368, 1986PubMedGoogle Scholar
  5. Elliot RB, Martin JM, Dietary protein: a trigger of insulin dependent diabetes mellitus. Diabetologia 26:297–299, 1984PubMedGoogle Scholar
  6. Elliot RB, Reddy SN, Bibby NJ, Kida K, Dietary prevention of diabetes in the non-obese diabetic mouse. Diabetologia 31:62–64, 1988PubMedGoogle Scholar
  7. Fajans SS, Floyd JC, Stimulation of islet cell secretion by nutrients and by gastrointestinal hormones released during ingestion. In: Greep RO, Astwood EB (eds) Handbook of physiology and endocrinology, Sect 7, Vol 1. American Physiology Society, Washington DC, pp473–494, 1972Google Scholar
  8. Hoorfar J, Scott FW, Cloutier HE, Dietary plant materials and development of diabetes in the BB rat. J Nutr 121:908–916, 1991PubMedGoogle Scholar
  9. Hoorfar J, Buschard K, Dagnaes-Hansen F, Prophylactic nutritional modification of the incidence of diabetes in autoimmune nonobese diabetic (NOD) mice. Br J Nutr 69:597–607, 1993PubMedGoogle Scholar
  10. Kikutani H, Makino S, The murine autoimmune diabetes model: NOD and related strains. Adv Immunol 51:285–322, 1992PubMedGoogle Scholar
  11. Leiter HE, Coleman DL, Eisenstein AB, Strack I, Dietary control of pathogenesis in C57B1/KsJ db/db diabetes mice. Metabolism 30: 554–562, 1981PubMedGoogle Scholar
  12. Lenzen S, Insulin secretion by isolated perfused rat and mouse pancreas. Am J Physiol 236:E391-E400, 1979PubMedGoogle Scholar
  13. Leslie RDG, Elliot RB, Perspectives in diabetes. Early environmental events as a cause of IDDM. Evidence and implications. Diabetes 43:843–850, 1994PubMedGoogle Scholar
  14. Linn T, Noke M, Woehrle M, Kloer HU, Bretzel RG, Federlin K, Diets different in free fatty acid intake modify the incidence of diabetes in a drug induced immune-mediated animal model. Aktuel Ernährungsmed 14:47–48, 1988Google Scholar
  15. Lovejoy J, DiGirolamo M, Habitual dietary intake and insulin sensitivity in lean and obese adults. Am J Clin Nutr 55:1174–1179, 1992PubMedGoogle Scholar
  16. Makino S, Kunimoto K, Muraoka Y, Mizushima Y, Katagiri K, Tochino Y, Breeding of a non-obese, diabetic strain of mice. Exp Anim 29:1–13, 1980Google Scholar
  17. Pozzilli P, Signore A, Williams JKA, Beales PE, NOD mouse colonies around the world — recent facts and figures. Immunol Today 14:193–196, 1992Google Scholar
  18. Scott FW, Marliss EB, Conference summary: diet as an environmental factor in development of insulin-dependent diabetes mellitus. Can J Physiol 69:311–319, 1991Google Scholar
  19. Scott FW, Kardish M, Hatina G, Trick KD, Wojcinski Z, Diet can prevent diabetes in the BB rat. Diabetes 34:1059–1062, 1985PubMedGoogle Scholar
  20. Siegel EG, Trapp VE, Wollheim CB, Renold AE, Schmidt FH, Beneficial effects of low-carbohydrate-high-protein diets in longterm diabetic rats. Metabolism 29:421–427, 1980PubMedGoogle Scholar
  21. Storlien LH, Jenkins AB, Laboratory chow-induced insulin resistance: a possible contributor to autoimmune type 1 diabetes in rodents (Letter). Diabetologia 39:618–619, 1996PubMedGoogle Scholar
  22. Storlien LH, Pan DA, Kriketos AD, Baur LA, High fat diet-induced insulin resistance: lessons and implications from animal studies. Ann N Y Acad Sci 683:82–90, 1993PubMedGoogle Scholar
  23. Zhong ZJ, Davidson L, Eisenbarth GS, Weiner HL, Suppression of diabetes in nonobese diabetic mice by oral administration of porcine insulin. Proc Nat Acad Sci USA 88:10252–10256, 1991PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Schneider
    • 1
  • H. Laube
    • 1
  • T. Linn
    • 1
  1. 1.Medical Clinic III and Policlinic, Department of Internal MedicineJustus Liebig UniversityGießenGermany

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