Die Wirkung von Prednisolon auf die Synthese von RNS und DNS in Phytohämagglutinin-stimulierten Lymphocyten

  • P. Drings
  • E. Fölsch
  • J. Drews
Article

Zusammenfassung

Prednisolonhemisuccinat hemmt in einer Konzentration von 30 μg/ml die Synthese von DNS und RNS in PHA-stimulierten Lymphocyten. Die Hemmung der DNS-Synthese ist abhängig vom Zeitpunkt der Zugabe des Steroids zum Kulturmedium und der Dauer seiner Einwirkung. Von der durch Prednisolon verursachten Verminderung der RNS-Synthese ist hauptsächlich die Neubildung von ribosomaler RNS betroffen. Das Sedimentationsmuster schnell markierter RNS aus PHA-stimulierten Lymphocyten enthält nach Einwirkung von Prednisolon keine bei 18 S sedimentierende radioaktive Komponente. Dieser Befund könnte auf der Synthese eines rudimentären Vorläufermoleküls für ribosomale RNS beruhen, in dem das 18 S-Teilstück nicht enthalten ist; er könnte andererseits mit einem beschleunigten Abbau dieses ribosomalen RNS-Anteils in Lymphocyten zusammenhängen, die in Gegenwart von Prednisolon inkubiert wurden.

Schlüsselwörter

Lymphocyten DNS-Synthese RNS-Synthese Prednisolon 

The effect of prednisolone on RNA- and DNA- synthesis in phytohemagglutinine-stimulated lymphocytes

Summary

The formation of DNA and RNA in PHA-stimulated human lymphocytes is inhibited by prednisolone-hemisuccinate introduced into the culture at a concentration of 30 μg/ml. The degree by which the steroid inhibits DNA synthesis depends on the time after stimulation at which it is added and on the duration of its presence in the cultures. The inhibition of RNA synthesis caused by prednisolone is largely due to a decrease in the formation of ribosomal precursor RNA. No radioactive 18 S peak was observed in the sedimentation profile of rapidly labeled RNA from cells treated with prednisolone. This finding could reflect the synthesis of a rudimentary ribosomal RNA precursor molecule which does not contain the 18 S RNA component; it could also be due to a greater instability of 18 S RNA in prednisolone-treated cells.

Key-words

Lymphocytes DNA Synthesis RNA Synthesis Prednisolone 

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Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • P. Drings
    • 1
  • E. Fölsch
    • 1
  • J. Drews
    • 1
    • 2
  1. 1.Medizinische Universitätsklinik (Ludolf Krehl-Klinik) HeidelbergDeutschland
  2. 2.Sandoz ForschungsinstitutWien

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