Oxidation of selected alkanes and related compounds by aPseudomonas strain
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The oxidation of octane and decane by a gram-negative bacterium, identified as aPseudomonas species, has been studied. The same rates of growth of the organism were observed in culture media supplemented with alkanes as sole source of carbon, irrespective of whether growth had previously taken place in media containing either octane or glucose. However, only cells previously grown in medium supplemented with octane oxidised this paraffin in the Warburg apparatus. Although 1-octene was not utilised for growth, the rate of oxidation of the olefin by resting cells was the same whether these were previously grown with octoic acid or with octane as sole source of carbon. Small amounts of 1-octanol and 1-octanal were oxidised by resting cells, but at higher concentrations respiration was inhibited.
The organism was grown at the expense of radioactive decane (l-C14) and at least half of the added substrate was oxidised to carbon dioxide. No evidence was found for the accumulation of fatty acids either in the cells or in the culture medium.
KeywordsGlucose Dioxide Carbon Dioxide Paraffin Decane
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- Breed, R. S., Murray, E. G. D. andParker Hitchens, A. 1948. Bergey's manual of determinative bacteriology, 6th ed. Ballière, Tindall and Cox, London.Google Scholar
- Foster, J. W. 1962b. Bacterial oxidation of hydrocarbons, p. 241.In O. Hayaishi, [ed.], Oxygenases. Academic Press, New York.Google Scholar
- Johnson, F. H., Goodale, W. T. andTurkevich, J. 1942. The bacterial oxidation of hydrocarbons. J. Cell. Comp. Physiol.19: 163–172.Google Scholar
- Kester, A. S. 1961. Studies on the oxidation of hydrocarbons by microorganisms. Ph. D. Thesis. Univ. of Texas.Google Scholar
- Kester, A. S. andFoster, J. W. 1960. Diterminal oxidation of long chain alkanes by a bacterium. Bacteriol. Proc.1960: 168.Google Scholar
- Robinson, D. S. 1961. Oxidation of selected hydrocarbons by a certain microorganism. Ph. D. Thesis. Univ. of Manchester.Google Scholar
- Sakami, W. 1955. Handbook of isotopic tracer methods. Dept. of Biochemistry, Western Reserve University, Cleveland, Ohio.Google Scholar
- Saz, A. K. 1949. The effect of long-chain compounds, particularly hydrocarbons, on the metabolism of tubercle bacilli. Arch. Biochem.22: 195–203.Google Scholar
- Shewan, J. M., Hodgkiss, W. andListon, J. 1954. A method for the rapid differentiation of certain non-pathogenic, asporogenous bacilli. Nature173: 208–209.Google Scholar
- Stanier, R. Y. 1947. Simultaneous adaptation: a new technique for the study of metabolic pathways. J. Bacteriol.54: 339–348.Google Scholar
- Thornley, M. J. 1960. The differentiation ofPseudomonas from other gram-negative bacteria on the basis of arginine metabolism. J. appl. Bacteriol.23: 37–52.Google Scholar
- Thijsse, G. J. E. andvan der Linden, A. C. 1958.n-Alkane oxidation by aPseudomonas. Studies on the intermediate metabolism. Antonie van Leeuwenhoek24: 298–308.Google Scholar
- Umbreit, W. W., Burris, R. H. andStauffer, J. F. 1957. Manometric techniques. Burgess Publishing Co., Minneapolis, Minn.Google Scholar
- Vandenheuvel, F. A. andHayes, E. R. 1952. Partition chromatography of aliphatic acids. Anal. Chem.24: 960–965.Google Scholar