Effect of mutation on DNA-composition of some bacteria
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The aim of this study was to investigate whether DNA from bacteria would be drastically changed by spontaneous and induced mutation. ‘Melting points’ of pure DNA were taken as a criterion to indicate possible changes. DNA from dark-blue Chromobacteria and from some pale-blue and non-pigmented spontaneous variants had the same ‘melting point’, indicating only a very small overall change in its base composition. A similar situation held forAcetobacter aceti (liquefaciens) and an Mn-induced mutant. A wild-typeAgrobacterium tumefaciens B6 and its streptomycin-resistant mutant S1 behaved similarly. However, pure DNA from another, UV-induced, mutant M39 had a ‘melting point’ which was 1.5 C higher, showing that considerable alterations in the structure of DNA may occur upon mutation. The results are discussed.
KeywordsMelting Point Base Composition Induce Mutation Spontaneous Variant Considerable Alteration
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- Béaud, G., Manigault, P. etStoll, Ch. 1963. Observations sur les tumeurs végétales incomplètes. Phytopathol. Z.47: 25–37.Google Scholar
- Doty, P., Marmur, J. andSueoka, N. 1959. The heterogeneity in properties and functioning of deoxyribonucleic acids. Brookhaven Symposia in Biol., No. 12; 1–16.Google Scholar
- Manigault, P. etBéaud, G. 1963. Sur certains des caractères distinctifs de mutants avirulents provenant d'une souche virulente d'Agrobacterium tumefaciens. Compt. rend.256: 2469–2470.Google Scholar
- Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol.3: 208–218.Google Scholar
- Marmur, J., Anderson, W. F., Matthews, L., Berns, K., Gajewska, E., Lane, D. andDoty, P. 1961. The effects of ultraviolet light on the biological and physical chemical properties of deoxyribonucleic acids. J. Cellular Comp. Physiol.58: Suppl. 1:33–55.Google Scholar
- Spirin, A. S., Belozersky, A. N., Kudlai. D. G., Skavronskaya, A. G. andMitereva, V. G. 1958. Changes in the nucleic acid composition as a result of experimental hereditary transformations of intestinal bacteria. Biochemistry (USSR) (Engl. Transl.)23: 163.Google Scholar