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Antonie van Leeuwenhoek

, Volume 30, Issue 1, pp 281–288 | Cite as

Effect of mutation on DNA-composition of some bacteria

  • J. De Ley
Article

Abstract

The aim of this study was to investigate whether DNA from bacteria would be drastically changed by spontaneous and induced mutation. ‘Melting points’ of pure DNA were taken as a criterion to indicate possible changes. DNA from dark-blue Chromobacteria and from some pale-blue and non-pigmented spontaneous variants had the same ‘melting point’, indicating only a very small overall change in its base composition. A similar situation held forAcetobacter aceti (liquefaciens) and an Mn-induced mutant. A wild-typeAgrobacterium tumefaciens B6 and its streptomycin-resistant mutant S1 behaved similarly. However, pure DNA from another, UV-induced, mutant M39 had a ‘melting point’ which was 1.5 C higher, showing that considerable alterations in the structure of DNA may occur upon mutation. The results are discussed.

Keywords

Melting Point Base Composition Induce Mutation Spontaneous Variant Considerable Alteration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Swets & Zeitlinger 1964

Authors and Affiliations

  • J. De Ley
    • 1
  1. 1.Laboratory for Microbiology, Faculty of SciencesState UniversityGentBelgium

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