Antonie van Leeuwenhoek

, Volume 30, Issue 1, pp 281–288 | Cite as

Effect of mutation on DNA-composition of some bacteria

  • J. De Ley


The aim of this study was to investigate whether DNA from bacteria would be drastically changed by spontaneous and induced mutation. ‘Melting points’ of pure DNA were taken as a criterion to indicate possible changes. DNA from dark-blue Chromobacteria and from some pale-blue and non-pigmented spontaneous variants had the same ‘melting point’, indicating only a very small overall change in its base composition. A similar situation held forAcetobacter aceti (liquefaciens) and an Mn-induced mutant. A wild-typeAgrobacterium tumefaciens B6 and its streptomycin-resistant mutant S1 behaved similarly. However, pure DNA from another, UV-induced, mutant M39 had a ‘melting point’ which was 1.5 C higher, showing that considerable alterations in the structure of DNA may occur upon mutation. The results are discussed.


Melting Point Base Composition Induce Mutation Spontaneous Variant Considerable Alteration 
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Copyright information

© Swets & Zeitlinger 1964

Authors and Affiliations

  • J. De Ley
    • 1
  1. 1.Laboratory for Microbiology, Faculty of SciencesState UniversityGentBelgium

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