Abstract
The preponderant pathway of octene-1 degradation by octane- and octene-1-grownPseudomonas aeruginosa cells (strain 473) starts with oxidation of the methyl group.
In addition, with both types of cells minor reactions occur that involve the double bond. The formation of a 1,2-epoxide was reported earlier. In addition, the identification of the saturated C8 fatty acid is a strong indication that the terminal methylene group is partially converted into an aldehydic group. The aldehyde seems to be formed beside the epoxide and the latter is not an intermediate. Enzymatic dihydroxylation of the double bond, if at all occurring, is masked by non-enzymatic hydrolysis of the epoxide.
The formation of a saturated methyl ketone could not be detected. Nor is the olefinic group converted into a primary or secondary alcohol group under conditions which result in accumulation of octanol-1 from octane and of 7-octenol-1 from octene-1.
The absence of a saturated alcohol among the intermediates in octene-1 degradation excludes hydration of the double bond as well as other mechanisms leading to saturated alcohols.
Accumulation of C8 fatty acids was effected by inhibition of β-oxidation with acrylate, whereas addition of a competing alcohol substrate (octanediol-1,8) yielded detectable amounts of the alcoholic intermediates.
Similar content being viewed by others
References
Azoulay, E., Chouteau, J. etDavidovics, G. 1963. Isolement et caractérisation des enzymes responsables de l'oxydation des hydrocarbures. Biochim. Biophys. Acta77: 554–567.
Baddiley, J., Buchanan, J. G., Handschumacher, R. E. andPrescott, J. F. 1956. Chemical studies in the biosynthesis of purine nucleotides. Párt I. The preparation ofn-glycylglycosylamines. J. Chem. Soc.1956: 2818–2823.
Beerstecher, E., Jr. 1954. Petroleum microbiology. Elsevier Press, Inc. New York and Houston.
Bruyn, J. 1954. An intermediate product in the oxidation of hexadecene-1 byCandida lipolytica. Koninkl. Ned. Akad. Wetenschap. Proc. Ser. C57: 41–45.
Foster, J. W. 1962. Bacterial oxidation of hydrocarbons, p. 241–271.In Hayaishi, O., [ed.], Oxygenases. Acad. Press. New York and London.
Ishikura, T. andFoster, J. W. 1961. Incorporation of molecular oxygen during microbial utilization of olefins. Nature192: 892–893.
van der Linden, A. C. 1963. Epoxidation of α-olefins by heptane-grownPseudomonas cells. Biochim. Biophys. Acta77: 157–159.
Metcalfe, L. D. andSchmitz, A. A. 1961. The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem.33: 363–364.
Stewart, J. E., Finnerty, W. R., Kallio, R. E. andStevenson, D. P. 1960. Esters from bacterial oxidation of olefins. Science132: 1254–1255.
Thijsse, G. J. E. 1964. Fatty acid accumulation by acrylate inhibition of β-oxidation in an alkane-oxidizingPseudomonas. Biochim. Biophys. Acta.84: 195–197.
Thijsse, G. J. E. andvan der Linden, A. C. 1958.n-Alkane oxidation by aPseudomonas. Studies on the intermediate metabolism. Antonie van Leeuwenhoek24: 298–308.
Thijsee, G. J. E. andZwilling-de Vries, J. T. 1959. The oxidation of straight and branched alkanes byPseudomonas strains. Antonie van Leeuwenhoek25: 332–336.
Thijsse, G. J. E. andvan der Linden, A. C. 1961.Iso-alkane oxidation by aPseudomonas. Part. I. Metabolism of 2-methylhexane. Antonie van Leeuwenhoek27: 171–179.
Thijsse, G. J. E. andvan der Linden, A. C. 1963. Pathways of hydrocarbon dissimilation by aPseudomonas as revealed by chloramphenicol. Antonie van Leeuwenhoek29: 89–100.
ZoBell, C. E. 1950. Assimilation of hydrocarbons by microorganisms. Advan. Enzymol.10: 443–486.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huybregtse, R., van der Linden, A.C. The oxidation of α-olefins by aPseudomonas reactions involving the double bond. Antonie van Leeuwenhoek 30, 185–196 (1964). https://doi.org/10.1007/BF02046725
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02046725