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Antonie van Leeuwenhoek

, Volume 34, Issue 1, pp 388–392 | Cite as

An easy screening assay for the enzymes of the Entner-Doudoroff pathway

  • K. Kersters
  • J. De Ley
Article

Abstract

An easy and quick assay is described for the semiquantitative determination of the key enzymes of the Enter-Doudoroff pathway: gluconate-6-phosphate dehydrase and 2-keto-3-deoxygluconate-6-phosphate aldolase. It is a micro-method, based on the thin-layer chromatographical identification of the reaction products after incubation of cell-free extracts with both substrates.

It is suitable for screening a large number of organisms.

Keywords

Pyruvate Aldolase Tetraacetate Ethylenediamine Tetraacetate Disodium Ethylenediamine Tetraacetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Swets & Zeitlinger 1968

Authors and Affiliations

  • K. Kersters
    • 1
  • J. De Ley
    • 1
  1. 1.Laboratory for Microbiology and Microbial Genetics, Facility of SciencesState UniversityGentBelgium

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