An easy screening assay for the enzymes of the Entner-Doudoroff pathway
An easy and quick assay is described for the semiquantitative determination of the key enzymes of the Enter-Doudoroff pathway: gluconate-6-phosphate dehydrase and 2-keto-3-deoxygluconate-6-phosphate aldolase. It is a micro-method, based on the thin-layer chromatographical identification of the reaction products after incubation of cell-free extracts with both substrates.
It is suitable for screening a large number of organisms.
KeywordsPyruvate Aldolase Tetraacetate Ethylenediamine Tetraacetate Disodium Ethylenediamine Tetraacetate
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