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Cytoplasmic fluorescence of thyroid follicle cells after incubation with thyroid stimulating hormone

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Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

Using the double antibody immunofluorescent technique on guinea pig thyroid sections after incubation with highly purified bovine TSH or with human serum, fluorescence appeared only in the cytoplasm of the follicular cells, pointing to probable fixation of TSH to ergastoplasmic membranes. The observation of nuclear fluorescence was not confirmed.

This technique apparently specific for TSH detection in biological fluids was unfit for a precise measurement of TSH concentration.

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Authors and Affiliations

  1. Laboratory of experimental medicine, University of Brussels, Belgium

    L. Vanhaelst & P. Neve

Authors
  1. L. Vanhaelst
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  2. P. Neve
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Additional information

We thank Mrs.Golstein (Laboratory of Nuclear Medicine, Brussels) for the gift of highly purified bovine TSH and for the help in performing radioimmunoassay, and Prof. P. A.Bastenie for his helpful criticisms.

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Vanhaelst, L., Neve, P. Cytoplasmic fluorescence of thyroid follicle cells after incubation with thyroid stimulating hormone. Z. Gesamte Exp. Med. 150, 1–6 (1969). https://doi.org/10.1007/BF02045834

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  • DOI: https://doi.org/10.1007/BF02045834

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