Abstract
Purified cell walls ofCandida albicans obtained from juvenile cells, mature yeast-like cells and filamentous cells were analyzed for their lipid components. Chloroform: methanol (2:1 v v) extraction of the acetone-treated dried cell walls indicated the total lipid content to be 2.1% of the dry weight of the juvenile cell walls, 1.8% of the mature yeast-like cell walls and 4.5% of the filamentous cell walls. Separation of the chloroform: methanol extractable fraction through a silicie acid column and quantitative determination of the fractions showed significant amounts of sterol esters, triglycerides, sterols, free fatty acids, and phospholipids in these extracts. Following acetone extraction sterols were shown to constitute a greater percentage of the cell wall of juvenile cells than mature cells. Thin-layer chromatography separated the acetone-extractable lipids into at least four components. Diethyl ether extracts of the cell walls indicated the presence of small amounts of glycerol phospholipids in the cell walls of juvenile and mature yeast cells. Boiling 95% ethanol also removed a small lipid fraction from the cell walls of both juvenile and mature yeast which could include sphingosine phosphatides or glycosides.
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References
Bianchi, D. E. 1964. Flocculation of lag phase yeast cells ofCandida albicans. Antonie van Leeuwenhoek30: 401–411.
Bianchi, D. E. 1965. Differential staining of yeast for purified cell walls, broken cells, and whole cells. Stain Technol.40: 79–82.
Di Salvo, A. F. andDinton, J. F. 1963. Lipid content of four strains ofBlastomyces dermatitudis of different mouse virulence. J. Bacteriol.85: 927–931.
Eddy, A. A. 1958. Structure of the yeast cell wall. II. Degradative studies with enzymes. Proc. Roy. Soc. (London). Ser. B.149: 425–440.
Elinov, N. P. andZaikina, N. A. 1963. Lipids of asporogenic yeast-like fungi of the genusCandida. Vopr. Med. Khim.9: 177–180.
Fillirup, D. L. andMead, J. F. 1953. Chromatographic separation of the plasma lipids. Proc. Soc. Exptl. Biol. Med.83: 574–577.
Hanahan, D. J. 1960. Lipide chemistry. John Wiley and Sons, New York.
Kessler, G. andNickfrson, W. J. 1959. Glucomannan-protein complexes from cell walls of yeasts. J. Biol. Chem.234: 2281–2285.
Masschillin, C. A. 1959. Structure of the cellular wall and mechanism of flocculation of bottom yeasts. Rev. Ferment. Ind. Aliment.14: 58–69, 87–112.
Mirdinger, E. andDevine, E. M. 1965. Lipids ofDebaryomyces hansenii. J. Bacteriol.89: 1488–1493.
Northcotf, D. H. andHorni, R. W. 1952. The chemical composition and structure of the yeast cell wall. Biochem. J.51: 232–236.
Peck, R. L. 1947. The lipids of fungi with special reference to pathogenic fungi. p. 167–188.In W. J. Nickerson, [ed.]. Biology of pathogenic fungi. Chronica Botanica Co., Waltham, Mass.
Pick, R. L. andHauser, C. R. 1939. Chemical studies of certain pathogenic fungi. II. The lipids ofMonilia albicans. J. Amer. Chem. Soc.61: 281–284.
Phah, H. J. 1963. Cell wall of yeasts. Ann. Rev. Microbiol.17: 15–30.
Sinthi Shanmufganathan, S. andNickerson, W. J. 1962. Composition of cells and cell walls of triangular and ellipsoidal forms ofTrigonopsis variabilis. J. Gen. Microbiol.27: 451–464.
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Bianchi, D.E. The lipid content of cell walls obtained from juvenile, yeast-like and filamentous cells ofCandida albicans . Antonie van Leeuwenhoek 33, 324–332 (1967). https://doi.org/10.1007/BF02045577
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DOI: https://doi.org/10.1007/BF02045577