Rubella virus interference and interferon production
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An interference technique for the titration of rubella virus on primary rabbit kidney (PRK) cells using either vaccinia or vesicular stomatitis virus (VSV) as challenge is described.
Rubella virus passed and propagated on PRK-cells interfered with a challenge dose of either vaccinia virus or VSV given on the 7th day after inoculation. Concomitantly, interferon was demonstrated in these cultures in amounts up to 100 units per ml.
The same strain of rubella virus passed on a continuous rabbit kidney cell line (RK13) induced no interferon in PRK-cells. Yet it interfered equally well with a challenge of vaccinia virus or VSV.
Under experimental conditions where no interferon could be detected, rubella virus growth inhibited the one-cycle multiplication of poliovirus-RNA, suggesting that interference resides at the intracellular level.
Under the described experimental conditions the multiplication of rubella virus in PRK-cells was not inhibited by an amount of interferon 20 times higher than that necessary to inhibit vaccinia virus cytopathic effect in the same cell system.
KeywordsInterferon Vaccinia Virus Kidney Cell Rubella Stomatitis
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- Baron, S. andWong, K. 1967. Role of interferon during rubella virus infection of green monkey kidney cell cultures, p. 656–658.In Proc. First Intern. Conf. Vaccines Viral Rickettsial Dis. Man. PAHO and WHO, Washington, Sci. Publ. No. 147.Google Scholar
- Günalp, A. 1965. Growth and cytopathic effect of rubella virus in a line of green monkey kidney cells. Proc. Soc. Exptl. Biol. Med.118 85–90.Google Scholar
- Holland, J. J., Hoyer, B. H., McLaren, M. C. andSyverton, J. T. 1960. Enteroviral ribonucleic acid. I. Recovery from virus and assimilation by cells. J. Exptl. Med.112 821–839.Google Scholar
- Kono, Y. andHo, M. 1965. The role of the reticuloendothelial system in interferon formation in the rabbit. Virology25 162–166.Google Scholar
- Lockart, R. Z., Jr. 1964. The necessity for cellular RNA and protein synthesis for viral inhibition resulting from interferon. Biochem. Biophys. Res. Commun.15 513–518.Google Scholar
- McCarthy, K., Taylor-Robinson, C. H. andPillinger, S. E. 1963. Isolation of rubella virus from cases in Britain. Lancet 1963,II 593–598.Google Scholar
- Parkman, P. D., Buescher, E. L. andArtenstein, M. S. 1962. Recovery of rubella virus from army recruits. Proc. Soc. Exptl. Biol. Meo.111 225–230.Google Scholar
- Plotkin, S. A. 1964. Plaqueing of rubella virus in RK13-cells. Arch. Ges. Virusforsch.16 423–425.Google Scholar
- Sever, J. L., Schiff, G. M. andTraub, R. G. 1962. Rubella virus. J. Am. Med. Assoc.182 663–671.Google Scholar
- Weller, T. H. andNeva, F. A. 1962. Propagation in tissue culture of cytopathic agents from patients with rubella-like illness. Proc. Soc. Exptl. Biol. Med.111 215–225.Google Scholar