Antonie van Leeuwenhoek

, Volume 33, Issue 1, pp 73–86 | Cite as

Iridescent material and the effect of iron on its production byPseudomonas aeruginosa

  • F. Wensinck
  • A. van Dalen
  • Martha Wedema
Article

Abstract

The nutritional conditions controlling iridescence inPseudomonas aeruginosa were studied using synthetic media solidified with agar. Iron and magnesium were growth-limiting factors in media solidified with dialysed agar. Iridescence only occurred on iron-deficient media and was not suppressed by adding Ca, Cu, Mn and Zn to these media. The ultraviolet absorption spectrum of the iridescent material was almost identical to the spectrum of the pyo I substances which are 2-alkyl-4-quinolinols.

The amount of material produced was inversely proportional to the iron content of the medium. Small amounts of material were produced by cells grown at levels of iron optimal for growth. Synthesis of 2-alkyl-4-quinolinol may be a normal metabolic process in the iridescent strains ofPseudomonas aeruginosa. It was enhanced by anthranilic acid and tryptophan; kynurenine and kynurenic acid had no effect. The results can be explained if it is assumed that the activity of iron-requiring enzymes catalizing the breakdown of tryptophan is reduced.

Even in the presence of anthranilic acid or tryptophan no material was produced by a non-iridescent strain.

Keywords

Iron Magnesium Agar Absorption Spectrum Tryptophan 

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Copyright information

© Swets & Zeitlinger 1967

Authors and Affiliations

  • F. Wensinck
    • 1
  • A. van Dalen
    • 1
  • Martha Wedema
    • 1
  1. 1.Laboratory of Bacteriology and SerologyState UniversityGroningenThe Netherlands

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