Versuche zur Beeinflussung der Antigenität von Homotransplantaten

Zusammenfassung

Es wurden Hauthomotransplantate bei Mäusen (C 57 Bl und AJ) vorgenommen. In der ersten Serie wurden die Transplantate während 12 Std in flüssigem Stickstoff gefroren, in der zweiten wurden sie in vitro mit 1500 r röntgenbestrahlt, und in der dritten Gruppe wurde den Empfängern √asylol (160000 E/kg) appliziert. Keine dieser Maßnahmen ergab eine signifikante Verlängerung des Transplantatüberlebens. In einer letzten Serie wurden Kaninchen 3 Tage vor bis 18 Tage nach Hauttransplantation mit Epsilonaminocapronsäure (2,5 mg/gi.p.) behandelt. Das Transplantatüberleben wurde dadurch um 6,8 Tage verlängert.

Summary

Skin homografts were performed on 350 mice of 0 57 Bl and AJ strains. The grafts of one group were stored in liquid nitrogen for a period of 12 hours and quickly rewarmed before transplantation. The first signs of rejection appeared around the edges on the 5th day, one day earlier than in the control group. Rejection was complete on the 9th day. There was microscopic evidence of lymphocytic infiltration as early as the 2nd day after grafting.

In the second group the grafts were radiated with 1500 r before transplantation. This pretreatment prolonged graft survival by 0.5 days. The lymphocytic infiltration was markedly diminished in comparison with the control group.

The third group of animals was treated with the proteinase inactivating substance trasylol (160000 U/kg i.p.) on the day of grafting and each of the following 4 days. The graft survival was prolonged by only 1.6 days in this group.

In the last series of experiments we grafted 20 rabbits and treated them with 2.5 mg/Gm i.p. epsilonaminocapronic acid daily from 3 days before to 18 days after the operation. The graft survival was prolonged by 6.8 days. In our experiments we found epsilonaminocapronic acid to be the only substance which prolonged homograft survival significantly without direct action upon the immunological system of the recipient.