Skip to main content
SpringerLink
Log in

Nucleinsäure- und Proteinstoffwechsel in Lymphocytenkulturen von Gesunden und Patienten mit Erkrankungen des lymphatischen Systems

I. Methodik und kinetische Untersuchungen bei Gesunden

Nucleic acid and proteinsynthesis of lymphocytes in vitro in normals and patients with lymphoproliferative disorders

I. Methods and results in normals

  • Published:
Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

The transformation to blast cells and the nucleic acid and protein synthesis of unstimulated and stimulated lymphocytes has been investigated in vitro. Unstimulated lymphocytes showed a low and relatively constant level of RNA and protein synthesis, whereas no DNA synthesis could be observed. PHA, Pokeweed, Streptolysin O and tuberculin stimulated normal lymphocytes exhibited a typical response in RNA, DNA and protein synthesis in cultures up to 9 days. With Pokeweed and specific mitogens the stimulation of DNA synthesis was much more pronounced as compared to RNA synthesis.

Zeammenfassung

Es wurde die Transformation zu Blastzellen und die Nucleinsäure- und Proteinsynthese unstimulierter und stimulierter Lymphocyten von Gesunden in vitro untersucht. Unstimulierte Lymphocyten zeigten einen niedrigen und relativ konstanten Basisstoffwechsel der makromolekularen RNS und der Zellproteine, ohne daß sich eine DNS-Synthese nachweisen ließ. Mit Phythämagglutinin, Pokeweed-Extrakt, Streptolysin O und Tuberkulin stimulierte normale Lymphocyten ließen einen typischen Ablauf ihrer RNS-, DNS- und Proteinsynthese in Kulturen bis zu 9 Tagen erkennen. Bei Pokeweed-Mitogen und spezifischen Mitogenen war die DNS-Synthese im Verhältnis zum RNS-Stoffwechsel stärker ausgeprägt.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Agrell, I. P. S.: Exp. Cell. Res.42, 403 (1966).

    Article  PubMed  CAS  Google Scholar 

  2. Allison, A. C., and L. Maluci: Lancet1964 II, 1371.

    Article  Google Scholar 

  3. Astaldi, G., G. Costa, and R. Airo: Lancet1965 I, 1394.

    Article  Google Scholar 

  4. Bach, F., and K. Hirschhorn: Exp. Cell. Res.32, 592 (1963).

    Article  PubMed  CAS  Google Scholar 

  5. — —: Sem. Hemat.2, 68 (1965).

    Google Scholar 

  6. Bender, M. A., and D. M. Prescott: Exp. Cell. Res.27, 221 (1962).

    Article  PubMed  CAS  Google Scholar 

  7. Berberg, H., H. Woodruff, R. Hirschhorn, B. Gerner, P. Miescher, and R. Silver: Science154, 1019 (1966).

    Article  PubMed  CAS  Google Scholar 

  8. Braunsteiner, H., R. Hofer u. S. Saller: Dtsch. med. Wschr.86, 723 (1961).

    Google Scholar 

  9. Caron, G. A.: A tritiate thymidine study of the effect of phythaemagglutinin on adult human skin in organ cultures. In: M. W. Elves: The biological effects of phythaemagglutinin, p. 151. Oswestry, England: The Robert Jones and Agnes Hunt Orthopaedic Hospital Management Committee, 1966.

    Google Scholar 

  10. Carstairs, K.: Lancet1962 I, 829.

    Article  Google Scholar 

  11. Chessin, L. N.: Persönliche Mitteilung.

  12. Cooper, E. H., P. Barkhan, and J. Halea: Brit. J. Haemat.9, 101 (1963).

    PubMed  CAS  Google Scholar 

  13. Cooper, H. L., and A. D. Rubin: Blood25, 1014 (1965).

    PubMed  CAS  Google Scholar 

  14. — —: Lancet1965 II, 723.

    Article  Google Scholar 

  15. Coulson, A. S., and D. G. Chalmers: Immunology12, 417 (1967).

    PubMed  CAS  Google Scholar 

  16. Cowling, D. C., D. Quaglino, and E. Davidson: Lancet1963 II, 1091.

    Article  Google Scholar 

  17. Ehrich, W., and T. N. Harris: J. exp. Med.90, 157 (1949).

    Article  CAS  PubMed  Google Scholar 

  18. Elves, M. W.: The lymphocytes. London: Lloyd Lake Ltd., 1966.

    Google Scholar 

  19. —, M. C. G. Israelis, and M. Collinge: Acta haemat. (Basel)37, 100 (1967).

    Article  CAS  Google Scholar 

  20. Epstein, L. B., and F. Stohlman: Blood24, 69 (1964).

    PubMed  CAS  Google Scholar 

  21. Feinendegen, L. E., V. P. Bond, and W. L. Hughes: Exp. Cell. Res.25, 627 (1963).

    Article  Google Scholar 

  22. — —, and R. B. Painter: Exp. Cell. Res.22, 381 (1961).

    Article  PubMed  CAS  Google Scholar 

  23. Fischer, R., A. Gropp u. H. Geiger: Klin. Wschr.44, 1366 (1966).

    Article  PubMed  CAS  Google Scholar 

  24. Fisher, D. B., and G. C. Mueller: Proc. Nat. Acad. Sci.60, 1396 (1968).

    Article  PubMed  CAS  Google Scholar 

  25. Fishman, M.: J. exp. Med.114, 837 (1961).

    Article  PubMed  CAS  Google Scholar 

  26. Fliedner, T. M., V. Kretschner, M. Hiller, and F. Wendt: Schweiz. med. Wschr.95, 1499 (1965).

    PubMed  CAS  Google Scholar 

  27. Gowans, J. L.: Brit. J. exp. Path.38, 67 (1957).

    PubMed  CAS  Google Scholar 

  28. —: J. Physiol. (Lond.)146, 54 (1959).

    CAS  Google Scholar 

  29. —: Ann. N.Y. Acad. Sci.99, 432 (1962).

    Article  PubMed  CAS  Google Scholar 

  30. —, and E. J. Knight: Proc. Roy. Soc.13, 159, 257 (1964).

    Google Scholar 

  31. Havemann, K., and A. D. Rubin: Proc. Soc. Exp. Biol. Med.127, 668 (1968).

    PubMed  CAS  Google Scholar 

  32. —, u. M. Schmidt: Klin. Wschr.46, 31 (1968).

    Article  PubMed  CAS  Google Scholar 

  33. Hirschhorn, K., F. Bach, R. L. Kolodney, I. L. Firschein, and N. Hashem: Science142, 1185 (1963).

    Article  PubMed  CAS  Google Scholar 

  34. Hirschhorn, R., K. Hirschhorn, and G. Weissmann: Blood30, 84 (1967).

    PubMed  CAS  Google Scholar 

  35. Hirschhorn, K., R. R. Schreibman, F. M. Bach, and L. E. Siltzbach: Lancet1964 II, 842.

    Article  Google Scholar 

  36. Holborow, E. J.: Lancet1967 I, 833, 890, 942, 995, 1049, 1098, 1148.

    Article  Google Scholar 

  37. Huber, H., C. Huber u. H. Braunsteiner: Dtsch. med. Wschr.91, 413 (1966).

    Google Scholar 

  38. Joachim, H. L.: Nature (Lond.)210, 919 (1966).

    Article  Google Scholar 

  39. McKinney, A. A.: Blood26, 36 (1965).

    Google Scholar 

  40. —, F. Stohlman, and G. Brecher: Blood19, 349 (1962).

    Google Scholar 

  41. Michalowsky, A., and J. Jasinka: Exp. Cell. Res.34, 417 (1964).

    Article  Google Scholar 

  42. Norman, A., M. S. Sasaki. R. E. Otoman, and A. G. Fingerhut: Science147, 745 (1965).

    Article  PubMed  CAS  Google Scholar 

  43. Nossal, G. J V.: Fact and fiction in immunological self-recognition mechanism. (J. G. Gibson Lecture, Columbia Presbyterian Medical Center.) New York: College Physicians and surgeons 1964.

    Google Scholar 

  44. Nowell, P. C.: Cancer Res.20, 462 (1960).

    PubMed  CAS  Google Scholar 

  45. Oppenheim, J. J.: Leucocyte culture conference Washington D.C., by McFarland, October 1965.

  46. Osgood, E. E., H. Tiney, K. B. Davidson, and A. J. Haman: Cancer5, 331 (1952).

    Article  PubMed  CAS  Google Scholar 

  47. Ottensen, J.: Acta physiol. scand.32, 75 (1954).

    Article  Google Scholar 

  48. Pogo, B. G. T., V. G. Allfrey, and A. E. Mirsky: Proc. Nat. Acad. Sci.55, 805 (1966).

    Article  PubMed  CAS  Google Scholar 

  49. Quaglino, D., F. G. J. Hayhoe, and R. J. Flemans: Brit. J. Haemat.10, 358 (1964).

    PubMed  CAS  Google Scholar 

  50. Rubin, A. D., and H. L. Cooper: Proc. Nat. Acad. Sci.54, 469 (1965).

    Article  PubMed  CAS  Google Scholar 

  51. —, K. Havemann, and W. Dameshek: Blood33, 313 (1969).

    PubMed  CAS  Google Scholar 

  52. Sabin, F. R.: J. exp. Med.70, 67 (1939).

    Article  CAS  Google Scholar 

  53. Salzman, N. P., M. Pellegrino, and P. Franceschini: Exp. Cell. Res.44, 73 (1966).

    Article  PubMed  CAS  Google Scholar 

  54. Sasaki, M. S., and A. Norman: Nature (Lond.)210, 913 (1966).

    Article  CAS  Google Scholar 

  55. Schmidt, M., u. K. Havemann: Verh. dtsch. Ges. inn. Med.74, 479 (1968).

    PubMed  CAS  Google Scholar 

  56. Schoenberg, M. D., V. R. Mumaw, R. D. Moore, and A. S. Weissberger: Science143, 964 (1964).

    Article  PubMed  CAS  Google Scholar 

  57. Seed, R. W., and I. H. Goldberg: Proc. Nat. Acad. Sci.50, 275 (1963).

    Article  PubMed  CAS  Google Scholar 

  58. Stanley, D. A., J. H. Frenster, and D. A. Rigas: J. Cell. Biol.39, 129a (1968).

    Article  Google Scholar 

  59. Thiele, H. G.: Verh. dtsch. Ges. inn. Med.73, 468 (1967).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medizinische Universitätsklinik Marburg a. d. Lahn, Deutschland

    K. Havemann

Authors
  1. K. Havemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Havemann, K. Nucleinsäure- und Proteinstoffwechsel in Lymphocytenkulturen von Gesunden und Patienten mit Erkrankungen des lymphatischen Systems. Z. Gesamte Exp. Med. 151, 138–162 (1969). https://doi.org/10.1007/BF02044668

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02044668

Key-words

Schlüsselwörter

Search

Navigation