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Die Zellspezifität heterologer, sogenannter antilymphocytärer Antiseren vom Kaninchen

The cellular specificity of heterologous, so-called anti-lymphocytic sera of the rabbit

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Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

The following types of antibodies were differentiated in heterologous rabbit antisera against mouse or rat lymphocytes: 1. Anti-erythrocyte/leucocyte/ thrombocyte/liver/kidney-antibody; 2. Anti-leucocyte/thrombocyte/liver/kidney-antibody; 3. Anti-leucocyte-antibody; 4. Anti-erythrocyte-antibody; 5. Anti-serum-protein-antibody.

While anti-erythrocyte and anti-leucocyte antibodies were found in the 7 S-as well as in the 19 S-Globulin-Fraction, antibodies against an antigen common to red cells and leucocytes were only demonstrated in the 7 S-fraction. The practical implications with the latter antibody what regards the absorption of red cell agglutinins were considered. Antibody activity against granulocytes was demonstrated by complementfixing tests, granulocyte count and extramedullary haemopoesis in the liver. The antibodies lack blood group specificity. Cross-reactivity between blood cells of rat and mouse does not involve all of the antibodies. The precipitation lines of the antibodies against organ extracts were not interpreted as organ specific.

Zusammenfassung

Im heterologen Antimäuse- bzw. Antirattenlymphocytenserum vom Kaninchen ließen sich die folgenden Antikörpertypen unterscheiden: 1. Anti-Erythrocyten/Leukocyten/Thrombocyten/Leber/Nieren-Antikörper; 2. Anti-Leukocyten /Thrombocyten/Leber/Nieren-Antikörper; 3. Antileukocytenantikörper; 4. Antierythrocytenantikörper; 5. Antiserumproteinantikörper.

Während antierythrocytäre und antileukocytäre Antikörper sowohl in der 7 S als auch in der 19 S-Globulin-Fraktion auftraten, wurden Antikörper, die gegen ein den Erythrocyten und Leukocyten gemeinsames Antigen gerichtet sind, nur in der 7 S-Fraktion nachgewiesen. Die Bedeutung des letzteren Antikörpertyps, insbesondere für die Absorption der Erythrocytenagglutinine, wird diskutiert.

Die gegen Granulocyten gerichtete Aktivität sogenannter antilymphocytärer Antikörper wird durch Komplementbindungsteste, Leukocytenkurven und den Nachweis extramedullärer sowie extrasplenaler Blutbildungszentren in der Leber wahrscheinlich gemacht. Neben dem Fehlen der Gruppenspezifität wird gezeigt, daß die Kreuzreaktion zwischen Blutzellen von Ratte und Maus auf einen Teil der Antikörper beschränkt ist.

Die gegen Organextrakte auftretenden Präzipitationen des Antilymphocytenserums werden als organunspezifisch interpretiert.

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Authors and Affiliations

  1. Institut für Hämatologie der GSF, Assoziation EURATOM, München

    S. Thierfelder, E. D. Möller, I. Kimura, P. Dörmer & M. Eulitz

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  1. S. Thierfelder
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  2. E. D. Möller
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  3. I. Kimura
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  4. P. Dörmer
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  5. M. Eulitz
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Studie im Rahmen der Assoziation Hämatologie EURATOM-GSF.

Fräulein U.Herrmann und Fräulein C.Speer danken wir für ihre wertvolle technische Mitarbeit.

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Thierfelder, S., Möller, E.D., Kimura, I. et al. Die Zellspezifität heterologer, sogenannter antilymphocytärer Antiseren vom Kaninchen. Z. Gesamte Exp. Med. 147, 44–59 (1968). https://doi.org/10.1007/BF02044590

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  • DOI: https://doi.org/10.1007/BF02044590

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