Blutlymphocyten im Licht- und Elektronenmikroskop nach Stimulation mit Phytohämagglutinin

I. Die Wirkung von Phytohämagglutinin auf Lymphocyten Gesunder
  • C. Orfanos
  • A. Pappas
  • P. G. Scheurlen
Article

Zusammenfassung

Kulturen von Blutlymphocyten mit und ohne Zusatz von Phytohämagglutinin (PHA) wurden im Licht-bzw. Elektronenmikroskop untersucht. Es fanden sich: a) kleine und mittelgroße Lymphocyten, b) große RNS-reiche, durch PHA stimulierte Zellen und c) Makrophagen. Die PHA-Zellen lassen sich lichtmikroskopisch als „blastenartig“ bezeichnen. Im Elektronenmikroskop ist ihre Morphologie nicht einheitlich. Wir fanden lymphoblasten- und immunoblastenähnliche PHA-Zellen. Zu den Makrophagen könnten fließende Übergänge bestehen. Offenbar nur ein Teil der in vitro entstehenden PHA-Zellen kann den in vivo, immunkompetenten“ Zellen gleichgesetzt werden.

Schlüsselwörter

Phytohämagglutinin Immunoblast Lymphoblast Lymphocyt Elektronenmikroskopie 

The blood lymphocytes under the light- and electron microscope after stimulation with phytohemagglutinin

I. the effect of phytohemagglutinin on lymphocytes of healthy individuals

Summary

Cultures of blood lymphocytes incubated with and without phytohemagglutinin (PHA) were examined under light- and electron microscope. The following types of cells were found: a) small and medium size lymphocytes, b) large, RNA-rich, stimulated cells and c) macrophages.

The PHA-cells of healthy individuals are large, showing a nucleus with nucleolus and a cytoplasma rich in free granules, but poor in ergastoplasma. Occasionally vacuoles of various sizes and osmiophilic inclusions are seen. Under light microscope these cells are similar to blastlike cells. Electron-microscopically the morphology of the PHA-stimulated cells is variable. We observed a) lymphoblast-like cells, b) immunoblast-like cells and c) macrophages. Our observations revealed, that only a part of the in vitro stimulated PHA-cells resembles immunocompetent cells. According to morphologic criteria the production of immunglobluins in vitro seems to be limited.

Key-words

Phytohemagglutinin Immunoblast Lymphoblast Lymphocyte Electron microscopy 

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Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • C. Orfanos
    • 1
    • 2
  • A. Pappas
    • 1
    • 2
  • P. G. Scheurlen
    • 1
    • 2
  1. 1.Hautklinik Universität KölnDeutschland
  2. 2.Medizinische Klinik Universität KölnDeutschland

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