Skip to main content
SpringerLink
Log in

Serumlipide, Blutgerinnung und Veränderungen an Blutgefäßen während intermittierender Cholesterinverfütterung an Kaninchen

  • Published:
Research in Experimental Medicine

Zusammenfassung

Bei 20 Kaninchen wurde eine intermittierende Cholesterinfütterung ohne sonstige Maßnahmen über 13 Monate durchgeführt. Während der Fütterungsperioden kam es zu einem Ansteigen des Cholesterins, der Phosphatide und der Neutralfette im Serum. Gerinnungsfaktoren und Fibrinolyse zeigten während der Lipämie keine signifikante Änderung. Die morphologische Gefäßuntersuchung der getöteten Tiere ließ im Verlauf des Versuches ein stetiges Fortschreiten erkennen. Im Vordergrund stand die Atherombildung mit oder ohne Calciumeinlagerung, Bindegewebsvermehrung und Fibrosierung der Gefäßwand. Vereinzelt wurden Mikroinfarkte beobachtet, jedoch fehlten Thrombosen und Hämorrhagien. Bei den am spätesten getöteten Tieren ließen die Atherome vereinzelt Aufbrüche erkennen. Die Ergebnisse zeigen, daß trotz der Unterbrechung der Cholesterinfütterung mit zunehmender Beobachtungsdauer die Gefäßwandveränderungen am deutlichsten ausgeprägt waren.

Summary

A cholesterol enriched diet was intermittantly given to 20 rabbits over a period of 13 months, without any other treatment.

During this time cholesterol, phosphatides and neutral lipids showed a tendency to increase, whereas no significant change was observed in the blood coagulation factors and fibrinolysis.

The histological investigation of the blood vessels showed a progressive development of atheromas with and without calcification, an increasing amount of connective tissue and fibrosis of the vascular wall. In some cases microinfarctions were found but no thrombosis or haemorrhagia.

The results indicate, that even an interrupted cholesterol feeding causes a striking change of the vascular lesions, when observed over a long period.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  • Anitschkow, N.: Über die Veränderungen der Kaninchenaorta bei experimenteller Cholesterinsteatose. Beitr. path. Anat.56, 379 (1913).

    Google Scholar 

  • —: Über die Atherosklerose der Aorta beim Kaninchen und über deren Entstehungsbedingungen. Beitr. path. Anat.59, 306 (1914).

    Google Scholar 

  • —: Zur Morphodynamik der Koronarsklerose des Herzens. Acta path. microbiol. scand.49, 426 (1960).

    Article  CAS  PubMed  Google Scholar 

  • Baratta, P. F., E. Mari eE. Rizzatti: Studio del complesso coagulativo del coniglio in conditioni di ateromasia sperimentale ipercolesteremica. Boll. Soc. ital. Biol. sper.27, 1412 (1951).

    CAS  PubMed  Google Scholar 

  • Beaumont, J.-L., Y. Grosgogeat, J. L. Richard etJ. R. Claude: La teneur en cholesterol de l'aorte et d'autres organes chez le lapin nourri avec du cholesterol. J. Atheroscler. Res.3, 210 (1963).

    Article  CAS  Google Scholar 

  • Billimoria, J. D., E. Lockey, andN. F. McLagan: Some observations on the effect of fats on blood clotting. Proc. roy. Soc. Med.50, 623 (1957).

    CAS  PubMed  PubMed Central  Google Scholar 

  • Borrero, I. E.: Further experiences with blood coagulation after fat meals and carbohydrate meals. Circulation17, 936 (1958).

    Article  CAS  PubMed  Google Scholar 

  • Clauss, A.: Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta haemat. (Basel)17, 237 (1957).

    Article  CAS  Google Scholar 

  • Constantinides, P.: Production of experimental atherosclerosis in animals. J. Atheroscler. Res.1, 374 (1961).

    Article  CAS  Google Scholar 

  • —, andR. N. Chakravarti: Rabbit arterial thrombosis production by systemic procedures. Arch. Path.72, 197 (1961).

    CAS  PubMed  Google Scholar 

  • —, andJ. Whyman: Infarction and infarctoid necrosis in atherosclerotic rabbits. J. Atheroscler. Res.2, 285 (1962).

    Article  CAS  PubMed  Google Scholar 

  • —J. Booth, andG. Carlson: Advanced atherosclerosis of the human type in the rabbit. Proceedings of the Symposion on Drugs affecting Lipid Metabolism, p. 277. Amsterdam: Elsevier Publ. 1960.

    Google Scholar 

  • — — —: Production of advanced cholesterol atherosclerosis in the rabbit. Arch. Path.70, 712 (1960).

    CAS  PubMed  Google Scholar 

  • Cook, R. P., A. Kliman, andL. F. Fieser: The absorption and metabolism of cholesterol and its main companions in the rabbit with observations on the atherogenic nature of the sterols. Arch. Biochem.52, 439 (1954).

    Article  CAS  PubMed  Google Scholar 

  • Dailey, I. P., E. A. Fullgrabe, R. L. Colescott, andZ. P. Tillotson: Lipidinduced coagulation changes in normal subjects and in patients with atherosclerosis. Amer. J. clin. Nutr.8, 34 (1960).

    CAS  PubMed  Google Scholar 

  • Davidson, E., A. N. Howard, andG. A. Gresham: The nature of coagulation defect in rats fed diets which produce thrombosis or experimental atherosclerosis. Brit. J. exp. Path.43, 166 (1962).

    CAS  PubMed  PubMed Central  Google Scholar 

  • Duff, G. L.: The nature of experimental cholesterol arteriosclerosis in the rabbit. Arch. Path.22, 161 (1936).

    CAS  Google Scholar 

  • Fiske, C. H., andY. Subbarow: The colorimetric determination of phosphorus. J. biol. Chem.66, 375 (1925).

    CAS  Google Scholar 

  • Folch, J., M. Lees, andG. H. S. Stanley: A simple method for the isolation and purification of total lipides from animal tissues. J. biol. Chem.226, 497 (1957).

    CAS  PubMed  Google Scholar 

  • Friedman, M., andS. O. Byers: Observations concerning the evolution of atherosclerosis in the rabbit after cessation of cholesterol feeding. Amer. J. Path.43, 301 (1963).

    Google Scholar 

  • Hartroft, W. S., andR. M. O'Neal: Coronary thrombosis; with reference to blood lipid levels in rats on an infarct-producing diet. Arch. Surg.82, 69 (1961).

    Article  CAS  PubMed  Google Scholar 

  • — —: Experimental production of coronary atherosclerosis. Amer. J. Cardiol.9, 355 (1962).

    Article  CAS  PubMed  Google Scholar 

  • Jobst, H., u. G.Schettler: Die chemische Zusammensetzung der Chylomikronen. III. Internat. Konf. über biochem. Probleme der Lipide, Brüssel 1956, S. 136.

  • Jürgens, J.: Neue Bestimmungsmethoden und Bedeutung der Antithrombine für die Klinik. Klin. Wschr.6, 303 (1957).

    Article  Google Scholar 

  • Keys, A., R. Buzina, F. Grande, andI. T. Anderson: Effect of meals of different fats on blood coagulation. Circulation15, 274 (1957).

    Article  CAS  PubMed  Google Scholar 

  • Kommerell, B.: The significance of blood coagulation in atherosclerosis. J. Atheroscler. Res.2, 233 (1962).

    Article  CAS  PubMed  Google Scholar 

  • —: Die Wirkung der Nahrungsfette auf die Blutgerinnung und die Beziehung zur Atherosklerose. Helv. med. Acta30, 177 (1963).

    CAS  PubMed  Google Scholar 

  • Kudrjashow, B. A., G. G. Bazasian, N. P. Sytina, andG. V. Andrcenko: Experimental prothrombotic state on organism resulting from the dysfunction of the physiological anticoagulating system induced by an atherogenic diet. Nature (Lond.)189, 67 (1961).

    Article  Google Scholar 

  • Lasch, H. G., u.Kl. Schimpf: Blutgerinnung u. alimentäre Fettbelastung. Dtsch. Arch. klin. Med.203, 146 (1956).

    CAS  PubMed  Google Scholar 

  • Manning, P. R., andB. L. Waldford: Lack effect of fat ingestion on blood coagulation. Lancet1958/II, 865.

    Google Scholar 

  • Marbet, R., u.A. Winterstein: Neuere Auffassung über den Mechanismus der Blutgerinnung. Experientia (Basel)10, 274 (1954).

    Article  Google Scholar 

  • McDonald, L., andM. Edgill: Coagulability of the blood in ischaemic heart-disease. Lancet.1957/II, 457.

    Article  Google Scholar 

  • — —: Changes in coagulability of the blood during various phases of ischaemic heart-disease. Lancet1959/I, 1115.

    Article  Google Scholar 

  • —, andH. W. Fullerton: Comparison of animal and vegetable fats in increasing blood coagulability. Lancet1958/II, 598.

    Article  Google Scholar 

  • McLagan, N. F., andI. D. Bilimoria: Food lipids and blood coagulation. Lancet1956/II, 235.

    Article  Google Scholar 

  • Mersky, C., N. Sapeika, C. J. Uys, andB. Bronte-Stewart: Experimentally induced atheroma in rabbits: effect of an anticoagulant drug (phenindione) on its development. S. Afr. J. Lab. clin. Med.5, 248 (1959).

    Google Scholar 

  • Mustard, J. F.: Increased activity of the coagulation mechanism during alimentary lipemia; its significance with regard to thrombosis and atherosclerosis. Canad. med. Ass. J.77, 308 (1957).

    CAS  PubMed  PubMed Central  Google Scholar 

  • —: Blood coagulation in subjects with and without clinical evidence of atherosclerotic vessel disease. Canad. med. Ass. J.79, 554 (1958).

    CAS  PubMed  PubMed Central  Google Scholar 

  • Naimi, S., R. Goldstein, M. M. Nothman, G. F. Wilgram, andS. Proger: Cardiovascular lesions and changes in blood coagulation and fibrinolysis associated with diet-induced lipemia in the rat. J. clin. Invest.41, 1708 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nath, M. C., andA. Saikia: Effects of essential fatty acids, inositol, Vit. B. 12 and hydrolyzed glucose-cyclo-acetoacetate on blood coagulation factors in rabbits exhibiting hyperhpemia induced by feeding saturated fat. J. Nutr.69, 403 (1959).

    CAS  PubMed  Google Scholar 

  • Nichols jr.,C. W., M. D. Siperstein, andI. L. Chaikoff: Effect of dihydrocholesterol administration on plasma cholesterol and atherosclerosis in the rabbit. Proc. Soc. exp. Biol. (N.Y.)83, 756 (1953).

    Article  CAS  Google Scholar 

  • Norman, P. S.: Studies of the plasmin system, I. Measurement of human and animal plasminogen. Measurement of an activator in human serum. J. exp. Med.106, 423 (1957).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • O'Brien, J. R.: Relation of blood coagulation to lipemia. Lancet1955/II, 690.

    Article  Google Scholar 

  • O'Neal, R. M., R. L. Tillmann, andW. A. Thomas: Butter, corn oil and fibrinolysis in rats. Circulation20, 26 (1956).

    Google Scholar 

  • Peyman, M. A., J. S. Nitzberg, R. Goldstein, M. Nothman, andS. Proger: The effect of longterm feeding of essential fatty acids on blood coagulation and fibrinolysis. Amer. J. Med.28, 884 (1960).

    Article  CAS  PubMed  Google Scholar 

  • Poole, I. C. F.: Fats and blood coagulation. Brit. med. Bull.14, 253 (1958).

    CAS  PubMed  Google Scholar 

  • Quick, A., Q. M. Stanley-Brown, andF. W. Bancroft: A study of the coagulation defect in hemophilia and in jaundice. Amer. J. med. Sci.190, 501 (1935).

    Article  Google Scholar 

  • Schoenheimer, R., andW. M. Sperry: A micromethod for the determination of free and combined cholesterol. J. biol. Chem.110, 655 (1935).

    Google Scholar 

  • Sharma, R., R. N. Chakravarti, andB. Mukeji: Investigations into some of the coagulation factors in cholesterol atherosclerosis of rabbits. Indian J. med. Res.44, 691 (1956).

    CAS  PubMed  Google Scholar 

  • Sheehy, T. W., andI. W. Eichelberger: Alimentary lipemia and the coagulability of blood. Circulation17, 927 (1958).

    Article  CAS  PubMed  Google Scholar 

  • Studer, A., u.K. Reber: Der Tierversuch in der Arterioskleroseforschung. Ergebn. allg. Path. path. Anat.43, 1 (1963).

    Google Scholar 

  • Tulloch, A. A., R. S. Overman, andJ. S. Wright: Failure of ingestion of cream to affect blood coagulation. Amer. J. Med.14, 674 (1953).

    Article  CAS  PubMed  Google Scholar 

  • Uhley, H. N., andM. Friedman: Blood lipids, clotting and coronary atherosclerosis in rats exposed to particular form of stress. Amer. J. Physiol.197, 396 (1959).

    CAS  PubMed  Google Scholar 

  • Wang, C., S. R. Drachman, andD. Adlersberg: Plasma lipid partition of the normal and cholesterol-fed rabbit. J. Mt Sinai Hosp.21, 19 (1954).

    CAS  Google Scholar 

  • Wilens, S. L.: The effect of chlorpromazine on arterial lipid deposition in rabbits after cholesterol feeding. Arch. Path.64, 570 (1957).

    CAS  Google Scholar 

  • Wolkoff, K.: Über die Veränderungen der Arterien bei Tieren. Virchows Arch. path. Anat.252, 208 (1924).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ludolf Krehl-Klinik, Medizinischen Universitätsklinik, Heidelberg

    Th. Pfleiderer, B. Kommerell, H. Wagener & W. Krauland

  2. Institut für Gerichtliche und Soziale Medizin der Freien Universität Berlin, Deutschland

    Th. Pfleiderer, B. Kommerell, H. Wagener & W. Krauland

Authors
  1. Th. Pfleiderer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Kommerell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Wagener
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Krauland
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Die Untersuchungen wurden durch das freundliche Entgegenkommen des Instituts für Tierzucht und Tierernährung der Freien Universität Berlin (Direktor: Prof. Dr.Bronsch) ermöglicht.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pfleiderer, T., Kommerell, B., Wagener, H. et al. Serumlipide, Blutgerinnung und Veränderungen an Blutgefäßen während intermittierender Cholesterinverfütterung an Kaninchen. Z. Gesamte Exp. Med. 139, 122–138 (1965). https://doi.org/10.1007/BF02043930

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02043930

Search

Navigation