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Differentiation of A-, B- and D-cells of guinea-pig pancreas using semi-thin epoxy-resin sections

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Summary

Four-mm2 specimens of guinea-pig pancreas were removed and fixed in buffered 5% glutaraldehyde, dehydrated through alcohols, treated with propylene oxide and impregnated with epoxyresin and finally polymerised at 70 °C. One-μ sections were cut on an ultramicrotome, stretched and adhered to glass slides. They were then deresinized in saturated sodium ethoxide, hydrated and washed thoroughly in running water. Sections were then stained by hematoxylin and eosin, Masson, Mallory, Epple, McGadey and the hematoxylin/acid fuchsin/orange G/soluble blue routines using the modified Weigert hematoxylin for the nuclear staining. They were then dehydrated, cleared and mounted in D.P.X. The stainings of the islet cells were compared and only by the latter routine did the D-cells stain adequately.

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References

  1. Aparicio S. R., Marsden P.: A rapid methylene blue-basic fuchsin stain for semi-thin sections of peripheral nerve and other tissues - J. Microsc. (Oxford)89, 139, 1969.

    Google Scholar 

  2. Aparicio S. R., Marsden P.: Application of standard micro-anatomical staining methods to epoxy resin-embedded sections - J. clin. Path.22, 589, 1969.

    PubMed  Google Scholar 

  3. Bailey G. W. (Ed.): Rapid infiltration of biological tissues utilising n-hexenyl succinic anhydride (HXSA) vinyl cyclohexene dioxide (VCD) an ultra-low viscosity embedding medium - 34th Ann. Proc. Electr.-Microsc. Soc. Amer., Claitors Publishing Division, Baton Rouge-Los Angeles, 1976; p. 346.

    Google Scholar 

  4. Beccari N.: Elementi di tecnica microscopica - Soc. Ed. Libraria, Milano 1952; p. 243.

    Google Scholar 

  5. Bennett S. H., Wyrick A. D., Lee S. W., McNeil J. H.: Science and art in preparing tissue embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains - Stain Technol.51, 71, 1976.

    PubMed  Google Scholar 

  6. Bensley R. R.: Studies on the pancreas of the guinea pig - Amer. J. Anat.12, 297, 1911.

    Article  Google Scholar 

  7. Bloom W.: A new type of granular cell in the islets of Langerhans of man - Anat. Rec.49, 363, 1931.

    Article  Google Scholar 

  8. Chang S. C.: Hematoxylin and eosin staining of plastic embedded sections - Arch. Path.93, 344, 1972.

    PubMed  Google Scholar 

  9. Epple A.: A staining sequence for A-, B-, and D-cells of the pancreatic islets - Stain Technol.42, 53, 1967.

    PubMed  Google Scholar 

  10. Glauert A. M., Glauert R. H.: Araldite as an embedding medium for electron microscopy - J. biophys. biochem. Cytol.4, 191, 1958.

    PubMed  Google Scholar 

  11. Glauert A. M., Rogers G. E., Glauert R. H.: A new embedding medium for electron microscopy - Nature (Lond.)178, 803, 1956.

    Google Scholar 

  12. Gomori G.: Studies of the cells of pancreatic islets - Anat. Rec.74, 439, 1939.

    Article  Google Scholar 

  13. Grimley P. M., Albrecht J. M., Michelitch H. J.: Preparation of large sections for light microscopy as an adjunct to fine-structure studies - Stain Technol.40, 357, 1965.

    PubMed  Google Scholar 

  14. Hoff A. F., Rayburn C.: A modified alcian blue-periodic acid Schiff stain for epoxy-embedded atherosclerotic arteries - Stain Technol.40, 241, 1974.

    Google Scholar 

  15. Humber J. D., Parker F., Odland G. F.: A basic fuchsin and alkalinized methylene blue rapid stain for epoxy embedded tissue - Stain Technol.43, 83, 1968.

    PubMed  Google Scholar 

  16. Kito H., Hosoda S.: Triple staining for simultaneous visualization of cell types in islets of Langerhans of pancreas - J. Histochem. Cytochem.25, 1019, 1977.

    PubMed  Google Scholar 

  17. Lane B. P., Europa D. L.: Differential staining of ultra-thin sections on epoxy-embedded tissue for light microscopy - J. Histochem. Cytochem.13, 579, 1965.

    PubMed  Google Scholar 

  18. Lumsden C. E.,Aparicio S. R.: The preparation of ultra-thin sections for light microscopy - J. clin. Path. (ACP) Broadsheet 72, Dec. 1970.

  19. Maldonado R., San José H.: A phloxine-azur-hematoxylin sequence for differential staining of cells in pancreatic islets - Stain Technol.42, 215, 1967.

    Google Scholar 

  20. Mayor H. D., Hampton J. C., Rosario B.: A simple method for removing the resin from epoxy embedded tissue - J. biophys. biochem. Cytol.9, 909, 1961.

    PubMed  Google Scholar 

  21. McGadey J.: Bulk staining method for alpha and beta cells of the pancreatic islets - J. med. Lab. Technol.27, 310, 1970.

    PubMed  Google Scholar 

  22. McGadey J.: A staining sequence for the differentiation of A-, B-, and D-cells of the islets of guinea-pig pancreas - Acta diabet. lat.16, 243, 1979.

    Google Scholar 

  23. Pool C. R.: Hematoxylin and eosin staining of OsO4fixed epoxy-embedded tissue, prestaining oxidation by acidified H2O2 - Stain Technol.44, 75, 1969.

    PubMed  Google Scholar 

  24. Pool C. R.: Prestaining oxidation by acidified H2O2 for revealing Schiff-positive sites in epoxyembedded sections - Stain Technol.48, 123, 0000.

  25. Rona G., Morvay I.: Differentiation of cells in the hypophysis and pancreatic islets - Stain Technol.31, 215, 1956.

    PubMed  Google Scholar 

  26. Sato R., Shamoto M.: A simple rapid polychrome stain for epoxy-embedded tissue - Stain Technol.48, 223, 1973.

    PubMed  Google Scholar 

  27. Schweisthal M. R., Frost C. C., Brinn J. E.: Stains for A, B and D-cells in fetal rat islets - Stain Technol.50, 161, 1975.

    PubMed  Google Scholar 

  28. Spurr A. R.: A low viscosity epoxy resin embedding medium for electron microscopy - J. Ultrastruct. Res.26, 31, 1969.

    Article  PubMed  Google Scholar 

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  1. Anatomy Department, University of Glasgow, Glasgow

    James McGadey & Owen Reid

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  1. James McGadey
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  2. Owen Reid
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McGadey, J., Reid, O. Differentiation of A-, B- and D-cells of guinea-pig pancreas using semi-thin epoxy-resin sections. Acta diabet. lat 18, 329–334 (1981). https://doi.org/10.1007/BF02042816

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  • DOI: https://doi.org/10.1007/BF02042816

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