Abstract
Laser angioplasty involves intravascular laser irradiation which may affect platelets and aggregation. In this study we examined the in vitro effects of different energy doses and modes of CO2 laser irradiation on the platelet number, function and ultrastructure. Since aspirin is used in many patients suffering from coronary artery disease, the consequences of laser irradiation on aspirin-treated platelets were studied as well.
We found that CO2 laser irradiation causes a dose-response reduction of the platelet number that is most pronounced with high energy-short duration irradiation, and a dose-response reduction in platelet aggregation which is independent of the mode of irradiation. Pretreatment with aspirin does not change the effects of laser irradiation on platelet number, while aggregation is reduced only at relatively high energy doses. Electron microscopy revealed significant intracellular injury following low energy irradiation, while progressive damage to membranes occurred in a dose-response mode, both in untreated and aspirin-treated platelets. The clinical application of our findings should be done cautiously since important in vivo factors, such as the effect of laser irradiation on the vessel wall and coagulation system, were not studied.
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References
Abela GS, Norman S, Cohen D et al. Effects of carbon dioxide, Nd-YAG and argon laser radiation on coronary atheromatous plaques.Am J Cardiol 1985,50:1199–205
Abela GS, Normann SJ, Cohen DM et al. Laser recanalization of occluded atherosclerotic arteries in vivo and in vitro.Circulation 1985,71:403–11
Gerrity RG, Loop FD, Golding LAR et al. Arterial response to laser operation for removal of atherosclerotic plaques.J Thorac Cardiovasc Surg 1983,85:409–21
Crea F, Davies G, McKenna W et al. Percutaneous laser recanalization of coronary arteries.Lancet 1982,ii:214–5
Ginsburg R, Wexler L, Mitchell RS, Profitt D. Percutaneous transluminal laser angioplasty for treatment of peripheral vascular disease: clinical experience with 16 patients.Radiology 1985,156:619–24
Crea F, Davies G, McKenna WJ et al. Laser recanalisation of coronary arteries by metal-capped optical fibres: early clinical experience in patients with stable angina pectoris.Br Heart J 1988,59:168–74
Wollenek G, Laufer G, Pinterits F et al. Excimer laser angioplasty: initial clinical results in total peripheral occlusion.Lasers Med Sci (Abstracts) 1988, abstr 330
Doerger PT, Glueck HI, Van Der Bel-Kahn J et al. The comparative effects of the argon, Nd-YAG, and argonpumped dye lasers on human platelets and erythrocytes in vitro.Lasers Surg Med 1985,5:457–68
Doerger PT, Glueck HI, McGill M. The effects of argon laser on in vitro aggregation of platelets in platelet rich plasma and whole blood.Thrombosis Res 1988,50:657–67
Eldar M, Gal D, Djaldetti M et al. Carbon dioxide laser effect on platelet function and surface ultrastructure in vitro.Lasers Surg Med 1988,8:259–63
Fisher JC. Interaction of laser light with living tissue. In:Vascular Interventional Laser: Physics, Safety and Clinical Applications. Proceedings of the Meeting of the Society of Cardiovascular and Interventional Radiology, Orlando, Florida, March 1988
Eldar M, Battler A, Gal D et al. The effects of varying lengths and powers of CO2 laser pulses transmitted through an optical fiber on atherosclerotic plaques.Clin Cardiol 1986,9:89–91
O'Brien JR. Effects of salicylates on human platelets.Lancet 1968, 779–83
Eldar M, Battler A, Neufeld HN et al. Transluminal CO2 laser catheter angioplasty.J Am Coll Cardiol 1984,3:135–7
Eldar M. Laser angioplasty: a review.Isr J Med Sci 1989,25:222–8