Journal of Radioanalytical and Nuclear Chemistry

, Volume 189, Issue 2, pp 175–182 | Cite as

Reactions of hydrated electron with hemoproteins in AOT reverse micelles

  • L. Gębicka
  • J. L. Gębicki


Reverse micelles formed from sodium bis (2-ethylhexyl)sulfosuccinate in n-heptane containing cytochrome c, horseradish peroxidase (HRP) and catalase in water pools preferentially scavenge electrons produced radiolytically in oil phase. Both initial absorbance at 720 nm and the half-lifetime of hydrated electron depend on the kind of hemoprotein and on the overall protein concentration. The possibility of electron capture by the protein molecule before its hydration is discussed. It is shown that the entrappment of cytochrome c and HRP into reverse micelles changes the reduction ability of the protein, whereas ferric iron in catalase remains unaccesible fore aq like in homogeneous aqueous solution.


Iron Hydrated Protein Concentration Catalase Horseradish Peroxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    J. H. FENDLER, Membrane-mimetic Chemistry, Wiley-Interscience, New York, 1982.Google Scholar
  2. 2.
    M. A. J. RODGERS, B. A. LINDIG, The Study of Fast Processes and Transient Species by Electron Pulse Radiolysis, D. Reidel Publishing Company, Dordrecht, 1982.Google Scholar
  3. 3.
    J. L. GĘBICKI, L. GĘBICKA, J. KROH, J. Chem. Soc. Faraday Trans., 90 (1994) 3411.CrossRefGoogle Scholar
  4. 4.
    M-P. PILENI, B. HICKEL, C. FERRADINI, J. PUCHEAULT, Chem. Phys. Lett., 118 (1982) 414.CrossRefGoogle Scholar
  5. 5.
    A. J. W. G. VISSER, J. H. FENDLER, J. Phys. Chem., 86 (1982) 947.CrossRefGoogle Scholar
  6. 6.
    M. WONG, M. GRÄTZEL, J. K. THOMAS, Chem. Phys. Lett., 30 (1975) 329.CrossRefGoogle Scholar
  7. 7.
    C. PETIT, P. BROCHETTE, M-P. PILENI, J. Phys. Chem., 90 (1986) 6517.CrossRefGoogle Scholar
  8. 8.
    R. BRU, P. WALDE, Eur. J. Bichem., 199 (1991) 95.CrossRefGoogle Scholar
  9. 9.
    S. KAROLCZAK, K. HODYR, R. ŁUBIS, J. KROH, J. Radioanal. Nucl. Chem., 101 (1986) 177.CrossRefGoogle Scholar
  10. 10.
    S. KAROLCZAK, K. HODYR, M. POLOWIŃSKI, Radiat. Phys. Chem., 39 (1992) 1.Google Scholar
  11. 11.
    W. H. KOPPENOL, K. J. H. van BUUREN, J. BUTLER, R. BRAAMS, Biochim. Biophys. Acta, 449 (1976) 157.PubMedGoogle Scholar
  12. 12.
    D. KEILIN, E. F. HARTREE, Biochem. J., 49 (1951) 88.Google Scholar
  13. 13.
    B. B. HASINOFF, Biochim. Biophys. Acta, 829 (1985) 1.Google Scholar
  14. 14.
    L. GĘBICKA, J. L. GĘBICKI, Radiat. Phys. Chem., 36 (1990) 161.Google Scholar
  15. 15.
    P. L. LUISI, M. GIOMINI, M-P. PILENI, B. H. ROBINSON, Biochim. Biophys. Acta, 947 (1988) 209.PubMedGoogle Scholar
  16. 16.
    P. BROCHETTE, C. PETIT, M-P. PILENI, J. Phys. Chem., 92 (1988) 3505.CrossRefGoogle Scholar
  17. 17.
    H. B. DUNFORD, Peroxidases in Chemistry and Biology, Vol. 2, CRC Press, Boca Raton, 1991.Google Scholar
  18. 18.
    N. KLYACHKO, N. G. BOGDANOVA, A. V. LEVASHOV, K. MARTINEK, Collect. Czech. Chem. Commun., 57 (1992) 625.CrossRefGoogle Scholar
  19. 19.
    M. R. N. MURTHY, T. J. REID III, A. SICIGNANO, N. TANAKA, M. G. ROSSMAN, J. Mol. Biol., 152 (1981) 465.CrossRefPubMedGoogle Scholar
  20. 20.
    P. L. LUISI, Kinetics and Catalysis in Microheterogenous Systems, Marcel Dekker Inc., New York, Basel, Hong-Kong, 1991.Google Scholar
  21. 21.
    J. HABER, P. MAŚLAKIEWICZ, J. RODAKIEWICZ-NOWAK, P. WALDE, Eur. J. Biochem., 217 (1993) 567.CrossRefPubMedGoogle Scholar
  22. 22.
    M. ADACHI, M. HARADA, J. Phys. Chem., 97 (1993) 3631.CrossRefGoogle Scholar
  23. 23.
    K. Y. LAM, J. W. HUNT, Int. J. Radiat. Phys. Chem., 7 (1975) 317.CrossRefGoogle Scholar
  24. 24.
    C. D. JONAH, J. R. MILLER, M. S. MATHESON, J. Phys. Chem., 81 (1977) 1618.CrossRefGoogle Scholar
  25. 25.
    Y. GAUDUEL, S. POMMERET, A. MIGUS, N. YAMADA, A. ANTONETTI, J. Optic. Soc. Am. B, 7 (1990) 1528.Google Scholar
  26. 26.
    M-P. PILENI, T. ZEMB, C. PETIT, Chem. Phys. Lett, 118 (1985) 414.CrossRefGoogle Scholar
  27. 27.
    L. GĘBICKA, J. L. GĘBICKI, Int. J. Radiat. Biol., 59 (1991) 617.PubMedGoogle Scholar
  28. 28.
    J. WILTING, K. J. H. van BUUREN, R. BRAAMS, B. F. van GELDER, Biochim. Biophys. Acta, 376 (1975) 285.PubMedGoogle Scholar

Copyright information

© Akadémiai Kiadó 1995

Authors and Affiliations

  • L. Gębicka
    • 1
  • J. L. Gębicki
    • 1
  1. 1.Institute of Applied Radiation ChemistryTechnical University of ŁódźŁódźPoland

Personalised recommendations