Journal of Radioanalytical and Nuclear Chemistry

, Volume 189, Issue 2, pp 175–182 | Cite as

Reactions of hydrated electron with hemoproteins in AOT reverse micelles

  • L. Gębicka
  • J. L. Gębicki
Article

Abstract

Reverse micelles formed from sodium bis (2-ethylhexyl)sulfosuccinate in n-heptane containing cytochrome c, horseradish peroxidase (HRP) and catalase in water pools preferentially scavenge electrons produced radiolytically in oil phase. Both initial absorbance at 720 nm and the half-lifetime of hydrated electron depend on the kind of hemoprotein and on the overall protein concentration. The possibility of electron capture by the protein molecule before its hydration is discussed. It is shown that the entrappment of cytochrome c and HRP into reverse micelles changes the reduction ability of the protein, whereas ferric iron in catalase remains unaccesible fore aq like in homogeneous aqueous solution.

Keywords

Iron Hydrated Protein Concentration Catalase Horseradish Peroxidase 

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Copyright information

© Akadémiai Kiadó 1995

Authors and Affiliations

  • L. Gębicka
    • 1
  • J. L. Gębicki
    • 1
  1. 1.Institute of Applied Radiation ChemistryTechnical University of ŁódźŁódźPoland

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