Effect of chlorsulfuron on phenylpropanoid metabolism in sunflower seedlings

  • Jeffrey C. Suttle
  • H. R. Swanson
  • D. R. Schreiner
Article

Abstract

The effect of the herbicide chlorsulfuron on phenylpropanoid titer and metabolism and the role of endogenous ethylene in this response was examined in light-grown sunflower (Helianthus annuus L.) seedlings. Application of chlorsulfuron to the apex resulted in large increases in both total phenolic and hydroxycinnamic acid content in hypocotyls isolated from the treated seedlings. Both of these parameters were increased within 24 h of herbicide treatment, and both reached a maximum level 3–4 days post-treatment. An increase in ethylene evolution was found to proceed in parallel with the alterations of phenolic content. The extractable activities of phenylalanine ammonia lyase,trans-cinnamic-4-hydroxylase, and soluble peroxidase were increased by chlorsulfuron treatment. Chlorsulfuron had little effect on total phenolic content when administered directly to isolated hypocotyl segments. Exogenous ethylene had no effect on the endogenous titer of phenolic compounds. Root-fed cobalt chloride (5 × 10−4 M) inhibited chlorsulfuron-induced ethylene production by 92% and also inhibited the accumulation of phenolic materials by 56%. Exogenous ethylene was unable to reverse the inhibition caused by cobalt chloride. It was concluded that chlorsulfuron-induced increases in phenolic compounds were not mediated solely by endogenous ethylene.

Keywords

Phenolic Content Phenylalanine Ammonia Lyase Hydroxycinnamic Acid Cobalt Chloride Ethylene Evolution 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Jeffrey C. Suttle
    • 1
  • H. R. Swanson
    • 1
  • D. R. Schreiner
    • 2
  1. 1.U.S. Department of Agriculture, Agricultural Research Service, Metabolism and Radiation Research LaboratoryState University StationFargoUSA
  2. 2.Department of BotanyNorth Dakota State UniversityFargoUSA

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