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Identification of gibberellins from sugarcane plants

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Abstract

Five GAs, GA1, GA3, GA19, GA20, and GA29, were identified in extracts from mature leaf and shoot apical meristem of flowering and non-flowering sugarcane (Saccharum spp. hybrids) by combined GC/MS. The presence of ABA was also confirmed.

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Abbreviations

eV:

electron volt

GA:

gibberellin

MS:

mass spectrometry

dw:

dry weight

G:

relative centrifugal force (gravity)

ABA:

abscisic acid

PVP:

insoluble polyvinylpyrrolidone

GLC:

gas liquid chromatography

GC/MS:

combined gas chromatography-mass spectrometry

MeTMSi:

methyl ester-trimethylsilyl ether

SIM:

single ion monitoring

RT:

retention time

TIC:

total ion current

TLC:

thin-layer chromatography

IR:

infrared

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Author notes

  1. J. A. Kuhnle

    Present address: Experiment Station Hawaiian Sugar Planters' Association, USDA/ARS, 96701, Aiea, Hawaii, USA

Authors and Affiliations

  1. Western Regional Research Center, USDA/ARS, 94710, Berkeley, California, USA

    J. A. Kuhnle & W. F. Haddon

  2. Experiment Station Hawaiian Sugar Planters' Association, USDA/ARS, 96701, Aiea, Hawaii, USA

    P. H. Moore & M. M. Fitch

Authors
  1. J. A. Kuhnle
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  2. P. H. Moore
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  3. W. F. Haddon
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  4. M. M. Fitch
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Additional information

Contribution from the USDA ARS and the Hawaiian Sugar Planters' Association Experiment Station, Aiea, Hawaii 96701 USA. Published with the approval of the Director as Paper No. 550 in the Journal Series of the Experiment Station, Hawaiian Sugar Planters' Association.

Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.

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Kuhnle, J.A., Moore, P.H., Haddon, W.F. et al. Identification of gibberellins from sugarcane plants. J Plant Growth Regul 2, 59–71 (1983). https://doi.org/10.1007/BF02042234

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  • DOI: https://doi.org/10.1007/BF02042234

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