Abstract
Treatment of germinating maize seedlings with 0.126 mM of the herbicide Perfluidone (Destun) (1,1,1-trifluoro-4′-[phenylsulfonyl]-methanesulfona-o-toluidide) for 2 days in the dark, then 3 days in the light, at 25°C causes decreases in fresh weight, dry weight, shoot length, and in total chlorophyll and carotenoid contents; in contrast, sunflower seedlings seem not to be affected. Perfluidone causes marked decreases in total lipids and in glyco- and phospholipids of maize seedlings. In sunflower cotyledons, total lipids and pigments (chlorophyll, carotenoids) are not affected, but there is an increase in glycolipids at the expense of phospholipids. After Perfluidone treatment, a significant increase in the fatty acid mole ratio (18∶0+18∶1+18∶2)/18∶3 was found for the maize glycolipids, monogalactosyl diacylglycerol (MGD), digalactosyl diacylglycerol (DGD), and sterol glycoside (SG) + esterified sterol glycoside (ESG), and for the phospholipid, phosphatidylcholine (PC). In sunflower seedlings, however, only the fatty acid mole ratio of ESG + SG showed an increase and that of phosphatidylserine (PS) showed a large decrease. The differential response of the two plant species to Perfluidone suggests that the control of linolenic acid biosynthesis may vary depending on plant species and/or on plant tissues.
Similar content being viewed by others
Abbreviations
- PG:
-
phosphatidylglycerol
- PC:
-
phosphatidylcholine
- PE:
-
phosphatidylethanolamine
- PI:
-
phosphatidylinositol
- PS:
-
phosphatidylserine
- MGD:
-
monogalactosyl diacylglycerol
- DGD:
-
digalactosyl diacylglycerol
- SQD:
-
sulfoquinovosyl diacylglycerol
- SG:
-
sterol glycoside
- ESG:
-
esterified sterol glycoside
- FA:
-
fatty acid
References
Bartels PG, McCullough C (1972) A new inhibitor of carotenoid synthesis in higher plants: 4-chloro-5-(dimethylamino)-2-α,α,α(trifluoro-m-tolyl)-3(2H)-pyridazinone (SANDOZ 6706). Biochem Biophys Res Commun 48:16–22
Bligh EC, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Christie WW (1973) Lipid analysis, isolation, separation and structural analysis of lipids. Pergamon Press, Oxford
Comar CL, Zschiele FP (1942) Analysis of plant extracts for chlorophylls a and b by a photoelectric spectrophotometric method. Plant Physiol 17:198–209
Evans RW, Kates M, Ginzburg N, Ginzburg BZ (1982) Lipid composition of halotolerant algae,Dunaliella parva Lerche andDunaliella tertiolecta. Biochim Biophys Acta 712:186–195
Ferrante G, Ohno Y, Kates M (1983) Influence of temperature and growth phase on desaturase activity of the mesophilic yeastCandida lipolytica. Can J Biochem Cell Biol 61:171–177
Kates M (1972) Techniques of lipidology. In: Laboratory Techniques in Biochemistry and Molecular Biology, Work TS, Work E (eds) Elsevier/North Holland, Amsterdam
St. John JB, Hilton JL (1973) Lipid metabolism as a site of herbicide action. Weed Sci 21:477–479
St. John JB (1982) Effects of herbicides on the lipid composition of plant membranes. ACS Symposium Series #181. Moreland DE, St. John JB, Hess FD (eds) Biochemical responses induced by herbicides. American Chemical Society
Valadon LRG, Mummery RS (1975) Carotenoids of floral parts and of the spadix ofArum maculatum. Z Pflanzenphysiol 75:88–94
Valadon LRG, Mummery RS (1977) Carotenoids of lilies and of red peppers. Z Pflanzenphysiol 82:407–416
Author information
Authors and Affiliations
Additional information
On leave from the Department of Botany, University of London, Royal Holloway College, Egham, Surrey TW20 OEX England
Rights and permissions
About this article
Cite this article
Valadon, L.R.G., Kates, M. Effect of perfluidone on metabolism of lipids in maize (Zea mays L.) and sunflower (Helianthus annuus L.). J Plant Growth Regul 3, 111–120 (1984). https://doi.org/10.1007/BF02041996
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02041996