Phytohormones,Rhizobium mutants, and nodulation in legumes. IV. Auxin metabolites in pea root nodules

  • Jane Badenoch-Jones
  • Roger E. Summons
  • Barry G. Rolfe
  • David S. Letham


High specific activity [3H]indole-3-acetic acid (IAA) was applied directly to root nodules of intact pea plants. After 24 h, radioactivity was detected in all plant tissues. In nodule and root tissue, only 2–3% of3H remained as IAA, and analysis by thin layer chromatography suggested that indole-3-acetyl-L-aspartic acid (IAAsp) was a major metabolite. The occurrence of IAAsp in pea root and nodule tissue was confirmed unequivocally by gas chromatography-mass spectrometry (GC-MS). The following endogenous indole compounds were also unequivocally identified in pea root nodules by GC-MS: IAA, indole-3-pyruvic acid, indole-3-lactic acid, indole-3-propionic acid, indole-3-butyric acid, and indole-3-carboxylic acid. Evidence of the occurrence of indole-3-methanol was also obtained. With the exception of IAA and indole-3-propionic acid, these compounds have not previously been unequivocally identified in a higher plant tissue.


Ethyl Acetate Fraction Indole Compound Nodule Extract Chemical Ionization Spectrum Thin Layer Chromatography System 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jane Badenoch-Jones
    • 1
  • Roger E. Summons
    • 1
  • Barry G. Rolfe
    • 1
  • David S. Letham
    • 1
  1. 1.Research School of Biological SciencesAustralian National UniversityCanberraAustralia

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