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Phytohormones,Rhizobium mutants, and nodulation in legumes. IV. Auxin metabolites in pea root nodules

  • Jane Badenoch-Jones
  • Roger E. Summons
  • Barry G. Rolfe
  • David S. Letham
Article

Abstract

High specific activity [3H]indole-3-acetic acid (IAA) was applied directly to root nodules of intact pea plants. After 24 h, radioactivity was detected in all plant tissues. In nodule and root tissue, only 2–3% of3H remained as IAA, and analysis by thin layer chromatography suggested that indole-3-acetyl-L-aspartic acid (IAAsp) was a major metabolite. The occurrence of IAAsp in pea root and nodule tissue was confirmed unequivocally by gas chromatography-mass spectrometry (GC-MS). The following endogenous indole compounds were also unequivocally identified in pea root nodules by GC-MS: IAA, indole-3-pyruvic acid, indole-3-lactic acid, indole-3-propionic acid, indole-3-butyric acid, and indole-3-carboxylic acid. Evidence of the occurrence of indole-3-methanol was also obtained. With the exception of IAA and indole-3-propionic acid, these compounds have not previously been unequivocally identified in a higher plant tissue.

Keywords

Ethyl Acetate Fraction Indole Compound Nodule Extract Chemical Ionization Spectrum Thin Layer Chromatography System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jane Badenoch-Jones
    • 1
  • Roger E. Summons
    • 1
  • Barry G. Rolfe
    • 1
  • David S. Letham
    • 1
  1. 1.Research School of Biological SciencesAustralian National UniversityCanberraAustralia

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