Gibberellic acid-induced cell elongation in cotton suspension cultures

  • Gayle H. Davidonis
Article

Abstract

Gibberellic acid (GA3) causes cell elongation in cotton suspension cultures derived from cotton ovule callus tissue of both auxin-dependent and-independent lines. Cell elongation was more pronounced in auxin-dependent cultures. Cells were cultured for a period of 14 days but differences in cell lengths could be detected after 6 days in culture. Cell elongation took place in cultures in which GA3 was present throughout the culture period or only for the first 3 days. Auxins and cytokinin alone or in the presence of GA3 did not promote cotton cell elongation above the value for the treatment with GA3 alone.

Keywords

Suspension Culture Gibberellin Cell Elongation Cell Length Cotton Ovule 

References

  1. Beasley CA, Ting IP (1973) The effects of plant growth substances onin vitro fiber development from fertilized cotton ovules. Am J Bot 60:130–139CrossRefGoogle Scholar
  2. Davidonis G (1989) Fiber development in preanthesis cotton ovules. Physiol Plant 75:290–294CrossRefGoogle Scholar
  3. Fry SC, Street HE (1980) Gibberellin-sensitive suspension cultures. Plant Physiol 65:472–477PubMedGoogle Scholar
  4. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  5. Grossmann K (1988) Plant cell suspensions for screening and studying the mode of action of plant growth retardants. Adv Cell Cult 6:89–136Google Scholar
  6. Jones RL (1980) The physiology of gibberellin-induced elongation. In: Skoog F (ed) Plant growth substances 1979. Springer-Verlag, Berlin, Heidelberg, New York, pp 188–195Google Scholar
  7. Jones RL, Moll C (1983) Gibberellin-induced growth in excised lettuce hypocotyls. In: Crazier A (ed) The biochemistry and physiology of gibberellins, vol. 2. Praeger, New York, pp 95–128Google Scholar
  8. Kaufman PB, Dayanandan P (1983) Gibberellin-induced growth inAvena internodes. In: Crozier A (ed) The biochemistry and physiology of gibberellins, vol. 2. Praeger, New York, pp 129–157Google Scholar
  9. Montague MJ, Ikuma H, Kaufman PB (1973) On the nature of the physiological responses ofAvena stem segments to gibberellic acid treatment. Plant Physiol 51:1026–1032PubMedGoogle Scholar
  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  11. Raskin I, Kende H (1984) The role of gibberellin in the growth response of submerged deep-water rice. Plant Physiol 76:947–950PubMedGoogle Scholar
  12. Silk WK, Jones RL, Stoddart JL (1977) Growth and gibberellin metabolism in lettuce hypocotyl sections. Plant Physiol 59:211–216PubMedCrossRefGoogle Scholar
  13. Trolinder NL, Berlin JD, Goodin JR (1987) Differentiation of cotton fibers from single cells in suspension culture. In Vitro Cell Develop Biol 23:789–794CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Gayle H. Davidonis
    • 1
  1. 1.Southern Regional Research CenterUSDA, ARSNew OrleansUSA

Personalised recommendations