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Characterization of C-11-SCH 23390 and its possible metabolites in primate blood using high performance liquid chromatography

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Abstract

A selective dopamine D1 antagonist, SCH 23390, is routinely labelled with C-11 at our institution for use in imaging dopamine D1 receptors in primate brain using positron emission tomography.However, little is known about the metabolism of this compound. In this report, a method is presented for the HPLC characterization of SCH 23390 and its possible metabolites in blood using a liquid-solid extraction technique. This procedure was applied to the analysis of blood samples collected at time intervals from rhesus monkeys after the injection of C-11-SCH 23390. About 4 minutes after injection, polar metabolite(s) were found in the circulation. However, the amounts detected would not be expected to interfere with the analysis of brain PET scans.

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Authors and Affiliations

  1. Positron Emission Tomography Center, Franklin McLean Research Institute The University of Chicago, 5841 South Maryland Avenue, 60637, Chicago, IL, (USA)

    O. T. De Jesus, M. L. Tokars, G. J. C. Van Moffaert, Y. W. Chen & M. D. Cooper

  2. Department of Pharmacological and Physiological Sciences, The University of Chicago, 5841 South Maryland Avenue, 60637, Chicago, IL, (USA)

    W. L. Woolverton

  3. Chemistry Division, Argonne National Laboratory, 60439, Argonne, IL, (USA)

    O. T. De Jesus

Authors
  1. O. T. De Jesus
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  2. M. L. Tokars
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  3. G. J. C. Van Moffaert
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  4. Y. W. Chen
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  5. W. L. Woolverton
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  6. M. D. Cooper
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De Jesus, O.T., Tokars, M.L., Van Moffaert, G.J.C. et al. Characterization of C-11-SCH 23390 and its possible metabolites in primate blood using high performance liquid chromatography. Journal of Radioanalytical and Nuclear Chemistry, Articles 125, 65–74 (1988). https://doi.org/10.1007/BF02041750

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  • DOI: https://doi.org/10.1007/BF02041750

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