Summary
The natural killer (NK) cell activity of unfractionated peripheral blood and synovial fluid mononuclear cells from patients with inflammatory joint disease was measured in a short-term assay using the human tumour cell line, K562, as the target. The mean values for peripheral blood NK activity of the various groups (controls, rheumatoid arthritis (RA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA)) were similar although the rheumatoid group showed the widest range. However, the NK activity of PsA patients (23.74 ± 10.14) was significantly lower than that of the controls (31.63 ± 10.8, 0.05, P>0.01). Almost without exception, NK activity was found to be considerably lower in synovial fluid than in paired blood samples (p<0.01).
Similar content being viewed by others
References
West, W.H., Cannon, G.B., Kay, H.D., Bonnard, G.D., Herberman, R.B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characteristics of effector cells. J Immunol, 1977, 118, 355–361.
Herberman, R.B., Djeu, J.Y., Kay, H.D., Ortaldo, J.R., Riccardi, C., Bonnard, G.D., Holden, H.T., Fagnani, R., Santoni, A., Puccetti, P. Natural killer cells: characteristics and regulation of activity. Immunol Rev, 1979, 44, 43–70.
Santoli, D., Trinchieri, G., Kaprowski, H. Cell-mediated cytotoxicity against virus-infected target cells in humans. II. Interferon induction and activation of natural killer cells. J Immunol, 1978, 121, 532–538.
Kiessling, R., Petranyi, G., Klein, G., Wigzell, H. Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non-immunised semi-syngeneic mice against a mouse lymphoma line. Int J Cancer, 1975, 15, 933–940.
Warner, N.L., Woodruff, M.F.A., Burton, R.C. Inhibition of the growth of lymphoid tumours in syngeneic (athymic) nude mice. Int J Cancer, 1977, 20, 146–155.
Hanna, N., Fidler, I.J. Role of natural killer cells in the destruction of circulating tumour emboli. JNCI, 1980, 65, 801–809.
Karre, K., Klein, G.O., Kiessling, R., Klein, G., Roder, J.C. Low natural in vivo resistance to syngeneic leukaemias in natural killer-deficient mice. Nature, 1980, 284, 624–626.
Talmadge, J.E., Meyers, K.M., Prieur, D.J., Starkey, J.R. Role of NK cells in tumour growth and metastasis in beige mice. Nature, 1980, 284, 622–624.
Collins, J.L., Patek, P.Q., Cohn, M. Tumorigenicity and lysis by natural killers. J Exp Med, 1981, 153, 89–106.
Haliotis, T., Roder, J., Klein, M., Ortaldo, J., Fauci, A.S., Herberman, R.B. Chediak-Higashi gene in humans. I. Impairment of natural-killer function. J Exp Med, 1980, 151, 1039–1048.
Trinchieri, G., Santoli, D. Antiviral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and an antagonistic inhibition of susceptibility of target cells to lysis. J Exp Med, 1978, 147, 1314–1333.
Gidmund, M., Orn, A., Wigzell, H., Senik, A., Gresser, I. Enhanced NK cell activity in mice injected with interferon and interferon-inducers. Nature, 1978, 273, 759–61.
Herberman, R.B., Ortaldo, J.R., Bonnard, G.D. Augmentation by interferon of human natural and antibody-dependent cell-mediated cytotoxicity. Nature, 1979, 277, 221–223.
Moore, M., Potter, M.R. Enhancement of human natural cell-mediated cytotoxicity by interferon. Br J Cancer, 1980, 41, 378–87.
Appleford, D.J.A., Denman, A.M. Fate of herpes simplex virus in lymphocytes from inflammatory joint effusions. I. Failure of virus to grow in cultured lyphocytes. Ann Rheum Dis, 1979, 38, 443–449.
Appleford, D.J.A., Denman, A.M. Fate of herpes simplex virus in lyphocytes from inflammatory joint effusions. II. mechanism of non-permissiveness. Ann Rheum Dis, 1979, 38, 450–455.
Penschow, J., MacKay, I.R. NK and K cell activity of human blood; differences according to sex, age and disease. Ann Rheum Dis, 1980, 39, 82–86.
MacLennan, I.C.M., Loewi, G. The cytotoxic activity of mononuclear cells from joint fluid. Clin Exp Immunol, 1970, 6, 713–720.
MacLennan, I.C.M., Loewi, G., The effect of peripheral lymphocytes from patients with inflammatory joint disease on human target cells in vitro. Clin Exp Immunol, 1968, 3, 385–391.
Burmester, G.R., Kalden, J.R., Peter, H.H., Schedel, I., Beck, P., Wittenborg, A. Immunological and functional characteristics of peripheral blood and synovial fluid lymphocytes from patients with rheumatoid arthritis. Scand J Immunol, 1978, 7, 405–417.
Boyum, A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest, 1968, 21 (suppl. 97), 77–89.
Timonen, T., Saksela, E., Rankin, A., Hayry, P. Fractionation, morphological and functional characterisation of effector cells responsible for human natural killer cell activity against cell-line targets. Cell Immunol, 1979, 48, 133–148.
Timonen, T., Ortaldo, J.R., Herberman, R.B. Characterisation of human granular lymphocytes and relationship to natural killer and K cells. J Exp Med, 1981, 153, 569–582.
Hoffman, T. Natural killer function in systemic lupus erythematosus. Arthritis Rheum, 1980, 23, 30–35.
Oshimi, K., Sumiya, M., Gonda, N., Kano, S., Takaku, F. Natural killer cell activity in systemic lupus erythematosus. Lancet, 1979, ii, 1023.
Goto, M., Tanimoto, K., Horiuchi, Y. Natural cell mediated cytotoxicity in systemic lupus erythematosus. Suppression by antilymphocyte antibody. Arthritis Rheum, 1980, 23, 1274–1281.
Gupta, S., Good, R.A. Subpopulation of lymphocytes. I. Studies in Immunodeficient Patients. Clin Exp Immunol, 1977, 30, 222–228.
Hooks, J.J., Moutsopoulos, H.M., Geis, S.A., Stahl, N.I., Decker, J.L., Notkins, A.L. Immune interferon in the circulation of patients with autoimmune disease. NEJM, 1979, 301, 5–8.
Hooks, J.J., Jordan, G.W., Cupps, T., Moutsopoulos, H.M., Fauci, A.S., Notkins, A.L. Multiple interferons in the circulation of patients with systemic lupus erythematosus and vasculitis. Arthritis Rheum, 1982, 25, 396–400.
Ytterberg, S.R., Schnitzer, T.J., Serum interferon levels in patients with systemic lupus erythematosus. Arthritis Rheum, 1982, 25, 401–406.
Dobloug, J.H., Forre, O., Kvien, T.R., Eegeland, T., Degre, M. Natural killer (NK) cell activity of peripheral blood, synovial fluid and synovial tissue lymphocytes from patients with rheumatoid arthritis and juvenile rheumatoid arthritis, Ann Rheum Dis, 1982, 41, 490–494.
Goto, M., Tanimoto, K., Chihara, T., Horiuchi, Y. Natural cell-mediated cytotoxicity in Sjogren's syndrome and rheumatoid arthritis. Arthritis Rheum, 1981, 24, 1377–1382.
Karsh, J., Dorval, G., Osterland, C.K., Natural cytotoxicity in rheumatoid arthritis and systemic lu pus erythematosus. Clin Immunol, Immunopathol 1981, 19, 437–446.
Silver, R.M., Redelman, D., Zvaifler, N.J. Naides, S. Studies of rheumatoid synovial fluic lymphocytes. I. Evidence for activated natural killer-(NK) like cells. J. Immunol., 1982, 128, 1758–1763.
Panayi, G.S., Corrigall, V. In: Immunopathoge nesis of rheumatoid arthritis. Eds. Panayi, G.S. Johnson, P.M. Reedbooks Ltd., Chertsey, Surrey 1979, 31–36.
Fox, R.I., Fong, S., Sabharwal, N., Carstens S.A., Kung, P.C., Vaughan, J.H. Synovial fluio lyphocytes differ from peripheral blood lympho cytes in patients with rheumatoid arthritis. J Immunol, 1982, 128, 351–354.
Fraser, J.R.E., Tait, B., Aaskon, J.G., Cunningham, A.L. Possible genetic determinants in epidemic polyarthritis caused by Ross River virus ir fection. Aust NZ J Med, 1980, 10, 597–603.
Armstrong, R.D., Panayi, G.S., Welsh, K.I. Histo compatibility antigens in psoriasis, psoriatic arthro pathy and ankylosing spondylitis. Ann Rheum Dis 1983, 42, 142–146.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Armstrong, R.D., Panayi, G.S. Natural killer cell activity in inflammatory joint disease. Clin Rheumatol 2, 243–249 (1983). https://doi.org/10.1007/BF02041398
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02041398