Experientia

, Volume 47, Issue 1, pp 67–68 | Cite as

Demonstration of fructose 1,6-bisphosphatase in human term placenta

  • T. Papamarcaki
  • O. Tsolas
Research Articles
  • 9 Downloads

Summary

A proteolysed form of fructose 1,6-bisphosphatase (Fru-P2ase) has been detected and characterized in human term placenta. The extract was found to contain very low levels of activity with an alkaline pH optimum. Western blotting demonstrated a polypeptide of Mr 26,000, instead of the subunit of Mr 36,000 observed in native mammalian Fru-P2ases.

Key words

Fructose 1,6-bisphosphatase human placenta 

Literatur

  1. 1.
    Horecker, B. L., Melloni, E., and Pontremoli, S., Adv. Enzymol.42 (1975) 193.Google Scholar
  2. 2.
    Tejwani, G. A., Adv. Enzymol.54 (1983) 121.Google Scholar
  3. 3.
    DiPietro, D. L., Gutierrez-Correa, J., and Thaidigsman, J. H., Biochem. J.103 (1967) 246.Google Scholar
  4. 4.
    Walker, D. G., Lea, M. A., Rossiter, G., and Addison, M. E. B., Archs Biochem. Biophys.120 (1967) 646.Google Scholar
  5. 5.
    Bagewadikar, R. S., Sharma, C., and Nadkarni, G. B., Indian J. exp. Biol.15 (1977) 27.Google Scholar
  6. 6.
    Bagewadikar, R. S., Sharma, C., and Nadkarni, G. B., Indian J. exp. Biol.15 (1977) 55.Google Scholar
  7. 7.
    Pontremoli, S., Traniello, S., Luppis, B., and Wood, W. A., J. biol. Chem.240 (1965) 3459.Google Scholar
  8. 8.
    Annamalai, A., Tsolas, O., and Horecker, B. L., Archs Biochem. Biophys.183 (1977) 48.Google Scholar
  9. 9.
    Bradford, M. M., Analyt. Biochem.72 (1976) 248.Google Scholar
  10. 10.
    Laemmli, U. K., Nature227 (1970) 680.Google Scholar
  11. 11.
    Towbin, H., Staehelin, T., and Gordon, J., Proc. natl. Acad. Sci. USA76 (1979) 4350.Google Scholar
  12. 12.
    Black, W. J., Van Tol, A., Fernando, J., and Horecker, B. L., Archs Biochem. Biophys.151 (1972) 576.Google Scholar
  13. 13.
    Engvall, E., and Perlmann, P., J. Immunol.109 (1972) 129.Google Scholar
  14. 14.
    Dzugaj, A., and Kochman, M., Biochim. biophys. Acta614 (1980) 407.Google Scholar
  15. 15.
    Lazo, P. S., Tsolas, O., Sun, S. C., Pontremoli, S., and Horecker, B. L., Archs Biochem. Biophys.188 (1978) 308.Google Scholar
  16. 16.
    Crivellaro, O., Lazo, P. S., Tsolas, O., Pontremoli, S., and Horecker, B. L., Archs Biochem. Biophys.189 (1978) 490.Google Scholar
  17. 17.
    Nakashima, K., and Ogino, K., J. Biochem. (Tokyo)75 (1974) 355.Google Scholar
  18. 18.
    Traniello, S., Pontremoli, S., Tashima, Y., and Horecker, B. L., Archs Biochem. Biophys.146 (1971) 161.Google Scholar
  19. 19.
    Byrne, W. L., Rajagopalan, G. T., Griffin, L. D., Ellis, E. H., Harris, T. M., Hochachka, P., Reid, L., and Geller, A. M., Archs Biochem. Biophys.146 (1971) 118.Google Scholar
  20. 20.
    Pontremoli, S., Melloni, E., Salamino, F., Sparatore, B., Michetti, M., and Horecker, B. L., Archs Biochem. Biophys.214 (1982) 376.Google Scholar
  21. 21.
    Taketa, K., and Pogell, B. M., Biochem. biophys. Res. Commun.12 (1963) 229.Google Scholar
  22. 22.
    Krebs, H. A., Proc. Roy. Soc. (London) Series B,159 (1964) 545.Google Scholar
  23. 23.
    Van Shaftingen, E., and Hers, H. G., Proc. natl Acad. Sci. USA.78 (1981) 2861.Google Scholar
  24. 24.
    Pilkis, S. J., El-Maghrabi, M. R., Pilkis, J., and Claus, T., J. biol. Chem.256 (1981) 3619.Google Scholar
  25. 25.
    Ghosh, N. K., and Fishman, W. H., J. biol. Chem.241 (1966) 2516.Google Scholar

Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • T. Papamarcaki
    • 1
  • O. Tsolas
    • 1
  1. 1.Laboratory of biological ChemistryUniversity of Ioannina Medical SchoolIoanninaGreece

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