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Replacement of native copper in superoxide dismutase with copper-64 without much loss in its enzymatic activity

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Abstract

Superoxide dismutase, containing copper and zinc, has been labeled with copper-64 by incubating the prepared apoenzyme with cupric(65) chloride at room temperature. No significant loss in the enzymatic activity was observed after labeling. The incorporation of copper-64 was ascertained by starch gel electrophoresis and high performance liquid chromatography. The labeling efficiency was found to be >95%.

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Authors and Affiliations

  1. Directorate of Nuclear Fuel and Materials, Pakistan Atomic Energy Commission, P. O. Box 1114, Islamabad, (Pakistan)

    S. Iqbal

  2. Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P. O. Nilore, Islamabad, (Pakistan)

    A. Naqvi & M. H. Rizvi

Authors
  1. S. Iqbal
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  2. A. Naqvi
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  3. M. H. Rizvi
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Iqbal, S., Naqvi, A. & Rizvi, M.H. Replacement of native copper in superoxide dismutase with copper-64 without much loss in its enzymatic activity. Journal of Radioanalytical and Nuclear Chemistry, Articles 159, 9–12 (1992). https://doi.org/10.1007/BF02041011

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  • DOI: https://doi.org/10.1007/BF02041011

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